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Mycolic Acid Methyl Esters
Reagent:
Mycolic acid methyl esters, MAME
Default Quantity:
1 mg
Production system:
Mycolic acids are released from the mAGP of M. tuberculosis by alkaline hydrolysis with KOH in methanol. The soluble mycolic acids are collected, and neutralized. Chloroform and water are added to the mycolic acid suspension to form a biphasic partition. The organic fraction containing mycolic acid is collected and dried.
Notes:
QC performed by TLC.
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