| Home | ||||||||||||
| Vaccine Testing |
||||||||||||
Research Materials
|
||||||||||||
| Links | ||||||||||||
| Contacts | ||||||||||||
| Frequently Asked Questions | ||||||||||||
| Publications | ||||||||||||
Cell Wall
Reagent:
Cell Wall Fraction, CW
Default Quantity:
1 mg
Production system:
Each strain is grown to late-log phase (day 14) in glycerol-alanine-salts (GAS) medium, washed with PBS pH 7.4 and inactivated by gamma-irradiation. The bacilli are suspended (2 g/ml) in PBS containing 8 mM EDTA, DNase, RNase and a proteinase inhibitor tablet, and broken in a French Press pressure cell at 4°C. Unbroken cells are removed by low speed (3,000 x g) centrifugation. The cell wall is isolated by centrifugation at 27,000 x g for one hour and washed 2 times in PBS. The final cell wall pellet is suspended and dialyzed in 0.01M ammonium bicarbonate, quantified by BCA protein assay for protein content, and stored at -80°C.
Notes:
This preparation contains proteins and non-protein compounds such as mAGP.
References:
Hirschfield, G.R. et al. J. Bacteriol. 172:1005, 1990.
Return to Subcellular Fractions | Return to Materials Available