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Inactivated Mycobacterium tuberculosis Whole Cells
Reagent:
Inactivated Mycobacterium tuberculosis Whole Cells, γ-irradiated whole cells
Default Quantity:
10 grams
Strains Available:
H37Rv, CDC1551, and HN878
Production system:
Each strain is grown to late-log phase (day 14) in glycerol-alanine-salts (GAS) medium. The bacilli are harvested by filtration washed with PBS pH 7.4, pelleted by centrifugation and frozen at -80°C. For inactivation, the cell pellet is exposed to 2.4 megaRads of ionizing gamma irradiation using a 137Cs source. Confirmation of inactivation is performed by Alamar Blue Assay. The inactivated cell pellets are stored at -80°C.
Notes:
A dose of 2.4 mRads of gamma irradiation kills M. tuberculosis to a 1020 degree of certainty while maintaining 93-95% of the biological activity of the enzymes.
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