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PhoS1
Reagent:
Rv0934, PhoS1, 38 kDa antigen, Anitgen 5, PstS1
Default Quantity:
250 µg
Production system:
The proteins soluble in 40% ammonium sulfate are precipitated with a 70% saturated ammonium sulfate solution. These proteins are suspended in ConA binding buffer and applied to a ConA column. ConA elution buffer, containing alpha-D-mannopyranoside, is used to elute the bound proteins. The bound material is applied to a phenyl sepharose column and eluted using a gradient of decreasing ammonium sulfate. Fractions containing pure 38kDa are pooled and dialyzed. Aliquots are stored at -80°C.
Notes:
It should be noted that the cell wall associated and culture filtrate forms of PhoS1 seem to differ in their hydrophobic characteristics. However, we have not yet determined if there is a chemical difference between the cell wall and culture filtrate forms of the PhoS1 homologue. The PhoS1 provided by this contract is purified from culture filtrate.