| Home | ||||||||||||
| Vaccine Testing |
||||||||||||
Research Materials
|
||||||||||||
| Links | ||||||||||||
| Contacts | ||||||||||||
| Frequently Asked Questions | ||||||||||||
| Publications | ||||||||||||
Alpha-Crystallin
Reagent:
Rv2031c, alpha-crystallin, 16 kDa antigen, HspX
Default Quantity:
100 µg
Production system:
Whole cell lysate is extracted with 0.1% n-octylthioglucoside in PBS and centrifuged at 27,000 xg for one hour. The supernatant is fractionated by isoelectric focusing (BioRad Rotofor system). Fractions containing 16kDa are pooled and cleaned up using size exclusion chromatography. Pure fractions of 16 kDa are pooled, dialyzed against 0.01 M ammonium bicarbonate, quantified using the BCA protein assay, lyophilized, and stored at -80°C.
References:
Lee B., et al. Infect. Immun. 60:2066, 1992.