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45 kDa Glycoprotein
Reagent:
Rv1860, 45 kDa glycoprotein, MPT32, ModD, Apa
Default Quantity:
500 µg
Production system:
CFPs are precipitated with a 40% saturated solution of ammonium sulfate. The precipitate is suspended and incubated with Conconavalin-A resin, and the 45 kDa is eluted with alpha-methyl mannoside. This fraction is concentrated and dialyzed. A final purification is performed using hydrophobic interaction (phenyl sepharose) HPLC. The purified 45 kDa glycoprotein is lyophilized and stored at -80°C.
Notes:
The 45 kDa glycoprotein appears as a doublet by SDS-PAGE. The major component is 45 kDa and the minor component is a 42 kDa degradation product. The mature MPT 32 glycoprotein has a calculated molecular weight of 30.2 kDa, however the abundance of proline residues along with glycosylation causes this molecule to migrate at 45 kDa by SDS-PAGE.
References:
Dobos K.M. et al., Infect. Immun. 63:2846, 1995.
Dobos K.M. et al., J. Bact. 178:2498, 1996.