Vol. 9 | No. 9
Dr. Rushika Perera, RNA Virologist, has accepted a tenure-track Assistant Professor position with MIP and will join the faculty on May 15, 2013
Congratulations to Dr Linda Vap and Dr Chad Frank for being promoted from Instructors to special appointment Assistant Professors effective December 1, 2012.
Each semester the MIP faculty recognize an outstanding graduating senior for academic excellence and/or service to the department or the community. Laylaa Ramos is the recipient of this award for the December 2012 graduating class.
Laylaa’s academic record is certainly superb, but that is not the only reason why she stands out in this field of truly outstanding individuals. In addition to being a full-time student, Laylaa has devoted a good deal of her time and talents to numerous service activities. She was an active member of the CVMBS College Council and a teaching assistant for the General Microbiology course. She also volunteered for Planned Parenthood and Camp Wapiyapi, a summer camp for children diagnosed with cancer. Laylaa was one of only a few undergraduate students chosen to participate in the Nicaragua Cook Stove Project, which is a study in which students help assess health risks associated with cooking indoors over open fires. Laylaa is also fluent in Spanish and has been using her bi-lingual skills to help translate the material from the Medical Virology lecture course for a graduate student from Mexico.
As if all this was not enough to keep Laylaa busy, she was also a research assistant for Dr. Glenn Telling in the Prion Research Center at CSU, and she obviously made quite an impression there! Dr. Telling wrote the following about Laylaa: "Laylaa is absolutely the best undergraduate student that I have ever had work in my group. She worked with us for a year on a complex research project that involved production and characterization of monoclonal antibodies against the prion protein. Laylaa is bright, self-motivated, responsible, enthusiastic, confident, punctual, hard-working and pleasant to be around. She also has excellent communication and technical skills." Laylaa presented her results of the study in a poster at the CSU Undergraduate Research Symposium and she is already a coauthor on an article published in the Journal of Biological Sciences, which is quite an accomplishment for an undergraduate researcher.
Laylaa intends to attend medical school and it is very likely that she will achieve that goal. Laylaa will be an outstanding physician – skilled, knowledgeable, and professional, but also warm, compassionate, and personable. And, of course, it is always beneficial for a physician to have a strong background in microbiology and infectious diseases!
Congratulations Laylaa and good luck in your future endeavors!
Congratulations to Anatomic Pathology Resident, Dr Alana Pavuk, for receiving the First Place Young Investigator Award at the ACVP Annual Meeting held Dec 1-5 in Seattle. Alana received the award for her research poster on mast cell tumors in Sharpeis. Anne Avery is her advisor.
Congratulations to Dr Paula Schaffer for receiving the CL Davis Student Scholarship Award this year for her accomplishments in superior scholarship, leadership, research ability and diagnostic skills. Paula received her award at the 63rd Annual Meeting of the American College of Veterinary Pathologists, December 1-5th in Seattle.
Congratulations to Jordan Steel, Graduate Student in Brian Geiss' Lab, for being accepted into ASM's first ever cohort of ASM-BWF Teaching Fellows. The ASM-BWF Science Teaching Fellows Program aims to help prepare doctoral-trained students for science teaching positions at a variety of non-doctoral institutions. Fellows in the program take part in a 10-month training experience that combines in-depth webinars, pre- and post-webinar assignments, structured mentoring, and a community of practice.
MIP Graduate Students who received their Degrees on December 14...
Amanda Calvert, PhD
MIP Undergraduate Students who received their B.S. Degrees on December 15...
In the News...
Microbiology undergraduate, Laylaa Ramos was highlighted in the Dec 10th edition of Today@Colorado State in the article, "Graduates overcome obstacles, prepare for real-world".
Justin Lee, BS, PhD Program, defended his dissertation entitled, " The Ecology and Evolution of Puma Lentivirus in Bobcats and Mountain Lions in North America" on December 6. His advisor is Dr. Sue VandeWoude.
Andrés Obregón-Henao, MD, MSc, PhD Program, defended his dissertation entitled, "Novel Extracellular Products of Mycobacterium tuberculosis: Composition, Synthesis, and Relevance to Disease" on December 6. His advisor is Dr. John Belisle.
Upregulation of the Phthiocerol Dimycocerosate Biosynthetic Pathway by Rifampin-Resistant, rpoB Mutant Mycobacterium tuberculosis
Greg Bisson, Carolina Mehaffy, Corey Broeckling, Jessica Prenni, Dalin Rifat, Desomnd Lun, Marcos Burgos, Drew Weissman, Petros Karakousis and Karen Dobos
J. Bacteriology 194: 6441-6452 (December 2012)
About 2 million people will die from tuberculosis (TB) this year. That’s about the same number of people who watched the Rockies play this year. Coincidence? Maybe not – after all both fields have someone named Belisle out there trying to keep things from getting out of hand. While figuring out how to make the Rockies successful is above our pay grade, one key reason that Mycobacterium tuberculosis (M. tb) is such a problem is drug resistance. M. tb. becomes resistant to the drug rifampin, a first line antibiotic, by mutating a region around the channel used by nucleic acids in the bacterial RNA Polymerase B subunit. If you think about it though, how can bacteria mutate something as fundamental as an RNA polymerase - a protein that makes its mRNAs - without killing itself? The answer must lie in compensatory mechanisms and/or mutations. Deep sequencing studies performed by a group across the pond at the MRC demonstrated that some strains compensate by mutating other components of the RNA polymerase to allow the altered polymerase complex to adapt to drug-resistant mutations in the B subunit. End of story – right? If it was you’d be reading about a different piece of excellent MIP science published this month.
Wisely not being content to think that nucleic acid sequences tell the whole story, Carolina, Karen, Corey, Jessica and their team of collaborators from around the planet set out to globally assess changes in proteins and metabolites in normal and rifampin-resistant M. tb. From the ~3,000 proteins they were able to analyze, interestingly about 10 proteins from the PDIM biosynthesis region of the genome were upregulated in rifampin-resistant strains. From the ~ 100 dysregulated compounds they were able to identify by metabolomic analysis, they identified numerous PDIM precursor molecules as well as peptidoglycan precursors. Thus this mountain of data actually came together in a very nice model. A natural defense mechanism of M. tb to rifampin is to upregulate a specific set of biosynthetic genes that likely have physiological relevance to help support the mutant organism and allow it to grow effectively.
So why did we choose this study as our last coveted MIPublication of the Month® for 2012? Three simple reasons my faithful reader. First, since environmental mycobacteria and other organisms appear to also upregulate these biosynthetic genes in a similar fashion, this study may very well have identified an attractive broad-spectrum antibiotic approach to lessen the chances of bacteria developing resistance to rifampin. Given the toll bacteria like M. tb have on humankind, this could be huge. Second, the study demonstrates the enormous value of the cutting edge technologies that we have here in MIP and in our neighboring Proteomics and Metabolomics (PMF) facility run by Jessica Prenni. Finally, the paper offers a rather unique challenge: I dare you to try to read the fourth word of the title of this paper without then feeling the need to clean spittle off of your computer monitor.
Best wishes to all for a lucky ’13.
MIP Publications Late November thru December 2012
García-Rejón JE, López-Uribe MP, Loroño-Pino MA, Arana-Guardia R, Puc-Tinal M, López-Uribe GM, Coba-Tún C, Baak-Baak CM, Machain-Williams C, Reyes-Solis GC, Lozano-Fuentes S, Saavedra-Rodriguez K, Black WC 4th, Beaty BJ, Eisen L. Aedes (Stegomyia) aegypti and Aedes (Howardina) cozumelensis in Yucatán State, México, with a summary of published collection records for Ae. cozumelensis. J Vector Ecol. 2012 Dec;37(2):365-72.
Larrouy-Maumus G, Skovierová H, Dhouib R, Angala SK, Zuberogoitia S, Pham H, Villela AD, Mikusová K, Noguera A, Gilleron M, Valentínová L, Korduláková J, Brennan PJ, Puzo G, Nigou J, Jackson M. A Small Multidrug Resistance-like Transporter Involved in the Arabinosylation of Arabinogalactan and Lipoarabinomannan in Mycobacteria. J Biol Chem. 2012 Nov 16;287(47):39933-41.
Grzegorzewicz AE, Korduláková J, Jones V, Born SE, Belardinelli JM, Vaquié A, Gundi VA, Madacki J, Slama N, Laval F, Vaubourgeix J, Crew RM, Gicquel B, Daffé M, Morbidoni HR, Brennan PJ, Quémard A, McNeil MR, Jackson M. A Common Mechanism of Inhibition of the Mycobacterium tuberculosis Mycolic Acid Biosynthetic Pathway by Isoxyl and Thiacetazone. J Biol Chem. 2012 Nov 9;287(46):38434-41.
Castillo EF, Dekonenko A, Arko-Mensah J, Mandell MA, Dupont N, Jiang S, Delgado-Vargas M, Timmins GS, Bhattacharya D, Yang H, Hutt J, Lyons CR, Dobos KM, Deretic V. Autophagy protects against active tuberculosis by suppressing bacterial burden and inflammation. Proc Natl Acad Sci U S A. 2012 Nov 13;109(46):E3168-76.
Bisson GP, Mehaffy C, Broeckling C, Prenni J, Rifat D, Lun DS, Burgos M, Weissman D, Karakousis PC, Dobos K. Upregulation of the Phthiocerol Dimycocerosate Biosynthetic Pathway by Rifampin-Resistant, rpoB Mutant Mycobacterium tuberculosis. J Bacteriol. 2012 Dec;194(23):6441-52.
Taylor JL, Wieczorek A, Keyser AR, Grover A, Flinkstrom R, Karls RK, Bielefeldt-Ohmann H, Dobos KM, Izzo AA. HspX-mediated protection against tuberculosis depends on its chaperoning of a mycobacterial molecule. Immunol Cell Biol. 2012 Nov;90(10):945-54.
Yang H, Kruh-Garcia NA, Dobos KM. Purified protein derivatives of tuberculin - past, present, and future. FEMS Immunol Med Microbiol. 2012 Dec;66(3):273-80.
Lozano-Fuentes S, Hayden MH, Welsh-Rodriguez C, Ochoa-Martinez C, Tapia-Santos B, Kobylinski KC, Uejio CK, Zielinski-Gutierrez E, Monache LD, Monaghan AJ, Steinhoff DF, Eisen L. The Dengue Virus Mosquito Vector Aedes aegypti at High Elevation in Mexico. Am J Trop Med Hyg. 2012 Nov;87(5):902-909.
Venable RO, Worley DR, Gustafson DL, Hansen RJ, Ehrhart EJ 3rd, Cai S, Cohen MS, Forrest ML. Effects of intratumoral administration of a hyaluronan-cisplatin nanoconjugate to five dogs with soft tissue sarcomas. Am J Vet Res. 2012 Dec;73(12):1969-76.
Kobylinski KC, Foy BD, Richardson JH. Ivermectin inhibits the sporogony of Plasmodium falciparum in Anopheles gambiae. Malar J. 2012 Nov 21;11(1):381.
Ruiz-Moreno D, Vargas IS, Olson KE, Harrington LC. Modeling dynamic introduction of chikungunya virus in the United States. PLoS Negl Trop Dis. 2012 Nov;6(11):e1918.
Bonizzoni M, Dunn WA, Campbell CL, Olson KE, Marinotti O, James AA. Complex Modulation of the Aedes aegypti Transcriptome in Response to Dengue Virus Infection. PLoS One. 2012;7(11):e50512.
Lipsitz R, Garges S, Aurigemma R, Baccam P, Blaney DD, Cheng AC, Currie BJ, Dance D, Gee JE, Larsen J, Limmathurotsakul D, Morrow MG, Norton R, O'Mara E, Peacock SJ, Pesik N, Rogers LP, Schweizer HP, Steinmetz I, Tan G, Tan P, Wiersinga WJ, Wuthiekanun V, Smith TL. Workshop on Treatment of and Postexposure Prophylaxis for Burkholderia pseudomallei and B. mallei Infection, 2010. Emerg Infect Dis. 2012 Dec;18(12):e2.
Rozovics JM, Chase AJ, Cathcart AL, Chou W, Gershon PD, Palusa S, Wilusz J, Semler BL. Picornavirus modification of a host mRNA decay protein. MBio. 2012 Nov 6;3(6). doi:pii: e00431-12.
The MIP Family once again showed it's generosity this holiday season by participating in The Salvation Army's Adopt-A-Family program. Giving Tree's decorated with the needs and wants of three local families were located in the Microbiology, Pathology and IDA Administrative Offices. The 'decorations' from each tree were completely gone by end-of-day December 6th, and the hearts of children in three local households will now be filled with the joy and magic of Christmas. THANK YOU to everyone that participated, especially Carol Wilusz, Karen Hofmaier, Cathy Griffin, and Tach Costello for their efforts in coordinating the donations.
The Annual MIP Thanksgiving Potluck was held on Friday, November 16. As is tradition, attendees enjoyed a delicious Thanksgiving meal with all the trimmings.
For a small 'taste' of the event, be sure to checkout the Thanksgiving Photo Gallery
The MIP Department Seminar Series will begin on Thursday, January 10th with a guest speaker hosted by Randy Basaraba. On February 18th Dr. Greg Brennan (hosted by Sue VandeWoude) will be presenting.
Check for updates on the MIP Department Seminar Schedule in January.
The Foothills Infectious Disease Seminar Series will kick-off the semester on January 17th with Dr. Barry Beaty presenting, "Dengue, Metabolites, Mosquitoes, and Nanoparticles: Compelling Reasons for Flunking Retirement".
SAVE THE DATE!
The 14th Annual CVMBS Research Day will be held on January 26, 2013 at the Hilton, Fort Collins.
The American Society for Microbiology is currently accepting applications for their ASM Undergraduate Research Fellowship
The deadline is February 1st!
The American Heart Association and the American Stroke Association undergraduate student research program is designed to inspire promising students from all disciplines, including women and members of minority groups underrepresented in the sciences, to consider research careers while supporting the highest quality scientific investigation broadly related to cardiovascular disease and stroke. The research opportunity will allow studentst to work for 10 weeks with a faculty/staff member on any project broadly related to cardiovascular function and diseases, stroke, or to associated basic science, clinical, bioengineering/biotechnology and public health problems.
To Apply Go To www.heart.org!
Look for Healthcare/Research Tab, Click on Research, then on Funding Opportunities.
The deadline is February 7th!
Hello. I’m Julie Iven, the new accounting tech for MIP, and I’ll be dividing my time between the Foothills Campus and the Microbiology Office. Before arriving here, I worked with student accounts at Front Range Community College (and still have ties back there as a part time accounting instructor), and also spent many years installing computer systems for banks in the UK. I’m the proud mother of two college students, one a senior at CSU. In my free time I’m remodeling my house, so if any of you have any great tips about plumbing, let me know. I’m looking forward to being part of the MIP team.
The Colorado Combined Campaign is one opportunity for CSU faculty and staff to support their favorite community charities via payroll deduction. If you are interested in participating you will need to do so by December 31. You can either donate online or with the paper pledge form you should have received in the mail. For more information, visit the Colorado Combined Campaign website.
Somehow between the ‘highs’ (e.g. Ammendment 64) and lows (e.g. High Park Fire, senseless theater shootings), MIP kept its focus and had another strong and interesting year. Here are the Top Ten Highlights as seen through the eyes of the MIPnews:
As we close the book on 2012, we are all looking forward to opening Gerry Callahan’s new book in the Spring. Best wishes to all of the MIP family for a great holiday season and a very Lucky ’13.
New Grant Awards
Karen Dobos, "Rif/INH Peptide Conjugation and Antibody Production", CDC Foundation.
Karen Dobos, "A Novel Proteomic Approach to TB Biomarker Discovery using Human Exosomes", Univeristy of California, San Francisco
Sebabrata Mahapatra, "Metabolomic Profiling of Serum and Urine ", Farmak
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