September 19, 2025 - Freezing, Handling & Storage of Stallion Semen; Laboratory Session Only
The frozen semen course is designed for anyone seriously interested in learning the techniques involved in freezing, thawing, and handling of equine spermatozoa.
COURSE IS FULL. To be placed on the waiting list, please email erl@colostate.edu with name, phone number and email address!
Course Description: It is required to register and complete the online Stallion Frozen Semen course prior to attendance. The online course covers all lectures and prerequisites needed to successfully complete the laboratory session.
The one-day course design will allow a more hands-on approach, enabling the attendee to efficiently freeze equine sperm. Small class sizes allow individual guidance. The course will emphasize a quality end product and simplify the management of the straws for future use. Participants will have a better understanding of the entire freezing process, quality control, storage, and frozen semen management. Hands-on laboratory sessions will provide an opportunity for participants to freeze, thaw and evaluate equine semen. Participants have ample time to work with and become comfortable with all the necessary pieces of equipment and products used. Specific laboratory topics include:
- Preparation of spermatozoa for a test freeze & methods for freezing equine sperm
- Response of spermatozoa to freezing and thawing
- Freezing extenders
- Proper handling, storage and thawing procedures
- Lab equipment requirements
- Proper use of cushion media
Additional topics we may be able to cover if time allows:
- Freezing sperm for ICSI use
- Epididymal sperm recovery and freezing
- Chemical ejaculation (for frozen semen)
- Fertility estimates of frozen semen
Previous experience in semen collection, evaluation, and insemination procedures or attendance at a CSU Equine Reproductive Management equine continuing education short course is required as a prerequisite for this course. Additionally, it is required to register and complete the online course on Stallion Frozen Semen.
- CE: 4 CE hours for veterinarians
Minimum Age for Course Attendance
We are committed to providing an educational and inclusive environment for all learners. However, due to the nature of the content in our Owner and Breeder courses, which includes discussions and images related to equine reproductive anatomy, breeding, and related medical procedures, we ask that any participants under the age of 18 be accompanied by a parent or legal guardian. We welcome young learners who have a sincere interest in equine science and are accompanied by an adult who can help contextualize and guide their experience.
Thank you for your understanding and support in maintaining a respectful and educational environment for all participants.
Courses held at the Equine Reproduction Laboratory
Colorado State University, 3101 Rampart Rd., Fort Collins, CO 80521
CSU ERL reserves the right to cancel course at any time.
