Total Lipid

Reagent:
Total Lipid

Default Quantity:
5 mg

Strains available:
H37Rv, HN878, and CDC 1551

Production system:
Each strain is grown to late-log phase (day 14) in glycerol-alanine-salts (GAS) medium, washed with PBS pH 7.4, inactivated by gamma-irradiation and dried. The cellular lipids are extracted with 30 ml of chloroform/methanol (2:1) per gram of cells at 55°C for 18 hr. Cells are removed by filtration and contaminating hydrophilic molecules are removed by biphasic partitioning with water (Folch wash). The organic phase of the folch wash is collected, dried and stored at -80°C.

Notes:
Total cellular lipids include those with known biological activities such as, trehalose dimycolate (TDM), and sulpholidids. Purification of individual lipids is done on a collaborative basis.

References:
Minnikin D.E., In Bacterial Cell Surface Techniques (I.C. Hancock and I.R. Paxton, Eds.) John Wiely & Sons, New York. pp 125-135, 1988.

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