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Attention

Research materials produced by the TBVTRM Contract are distributed by the Biodefense and Emerging Infections Research Resources Repository (BEI Resources). Please visit our page on the BEI Resources website for complete ordering instructions:
http://www.beiresources.org/TBVTRMResearchMaterials/tabid/1431/Default.aspx .

Trehelose Dimycolate

Reagent:
Trehelose Dimycolate, TDM

Default Quantity:
0.25 mg

Production system:
Trehalose dimycolate (TDM) is purified by extracting H37Rv Mtb cells with 2:1 chloroform/methanol and loading onto a silica gel column. The column is washed with chloroform, then eluted with 5% methanol in chloroform. TDM, enriched in this fraction, is further purified by loading onto C18 reverse phase SepPak filter and eluting with 60% chloroform in methanol.

Notes:
For most requests, this product will be made on demand; therefore, a delay should be anticipated.

 

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