TB Vaccine Testing and Research Materials Contract

	Materials Available

	Production Manuals & SOPs

Links

Contacts

Frequently Asked Questions

Publications

BEI Resources Transition

Attention

Research materials produced by the TBVTRM Contract are distributed by the Biodefense and Emerging Infections Research Resources Repository (BEI Resources). Please visit our page on the BEI Resources website for complete ordering instructions:
http://www.beiresources.org/TBVTRMResearchMaterials/tabid/1431/Default.aspx .

Production Manuals & SOPs

The documents provided on this page are in pdf format. If you do not have a pdf reader, download Adobe Reader now.

Production Manuals:: Large Scale Growth; Subcellular Fractions; Preparation and Fractionation of Cell Wall Proper; Lipoarabinomannan (LAM) and Lipomannan (LM); Antibodies; Microarrays

Individual SOPs:

Media: M001: Preparation of GAS medium; M002: Preparation of GAS medium with tween; M003: Preparation of Sauton's medium; M004: Preparation of Sauton's medium with tween; M005: Preparation of 7H9 medium; M006: Preparation of 7H9 medium with tween; M007: Preparation of 7H9-Dextrose medium; M008: Preparation of 7H9-Dextrose medium with tween; M009: Preparation of 7H11 agar plates; M010: Preparation of 7H11-Dextrose agar plates; M011: Preparation of Nutrient agar plates; M012: Preparation of Complete RPMI medium; M013: Preparation of Mycobactin HR Media and Plates
Reagents: R001: Preparation of 32% Triton; R002: Preparation of 1st fix for gels; R003: Preparation of 2nd fix for gels; R004: Preparation of Coomassie stain reagent; R005: Preparation of Coomassie stain destain; R006: Buffer B for polyacrylamide gels; R007: Buffer D for polyacrylamide gels; R008: Preparation of SDS-PAGE Tris-glycine running buffer; R009: SDS-PAGE 10X Tricine running buffer; R010: Preparation of western blot transfer buffer; R011: Preparation of charring spray; R012: Preparation of α-napthol spray; R013: Preparation of Dittmer-Lester spray; R014: Preparation of TAE Buffer
Native Material Production: PP001: Establishing frozen stocks of M. tb (small scale); PP002: Establishing frozen stocks of M. tb (large scale); PP003: Large scale growth of M. tuberculosis; PP004: Gamma-irradiation of M. tb; PP005: Preparation of CFPs and low MW CFPs; PP006: Preparation of CFPs using 2L Stirred Cell; PP007: Preparation of whole cell lysate; PP008: Preparation of subcellular fractions; PP009: Preparation of genomic DNA; PP010: Preparation of TX-114 proteins; PP011: Preparation of mAGP; PP012: Preparation of arabinagalactan; PP013: Preparation of peptidoglycan; PP014: Preparation of mycolic acids; PP015: Preparation of LAM, LM, and PIM (LLP); PP016: Separation of LLP; PP017: QC of LAM and LM; PP018: Isolation of total lipid; PP019: Purification of Con-A reactive CFPs; PP020: Preparation of Ag85 complex; PP021: Preparation of Ag85 A, B, and C; PP022: Purification of MPT32; PP023: Purification of the 16kDa ACR/HspX; PP024: Purification of 38kDa; PP025: Preparation of SDS soluble cell wall protein; PP026: Preparation of PIM; PP027: Purification of SapM; PP028: Preparation of CFP minus LAM; PP029: Purification of Trehalose Dimycolate (TDM); PP030: Purification of Trehalose Monomycolate (TMM); PP031: Purification of 19kDa; PP032: Purification of Mycobactin; PP033: Growth of M.tb for Mycobactin Production; PP034: Preparation of Sulfolipid-1
Recombinant Material Production: RP001: Generation of Recombinant Clone Frozen Stocks; RP002: Establishing Stocks of Low Copy Plasmids; RP003a: Production of Recombinant Proteins; RP003b: Production of Recombinant Proteins Denaturing Conditions; RP004: Production of ESAT6; RP005: Production of CFP10; RP006: Small- Scale Purification of Plasmid DNA; RP007: Production of rec GlcB; RP007msmeg: Production of rec GlcB from M. smegmatis; RP008: Production of rec Rv1411c; RP010: Production of rec Ag85A; RP011: Production of rec Ag85B; RP012: Production of rec Ag85C; RP014: Production of rec DnaK; RP015: Production of rec GroEL2; RP016: Production of rec Mpt64; RP019: Production of rec BRFB; RP020: Production of rec Mpt51; RP021: Production of rec HspX; RP022: Production of rec Rv2626c; RP023: Production of rec Rv0577; RP025: Production of rec KatG; RP026: Production of rec Rv3763; RP028: Production of GroES
Antibody Production: AB100: Cell Line Maintenance; AB101: IgG Purification; AB102: Antibody QC; AB103: Monoclonal Fusion; AB104: Screening and Subcloning; AB105: Freezing Cell Lines
Standard Procedures: SP003: BCA protein assay; SP004: Lyophilization; SP005: Operation of the savant; SP006: Preparation of SDS-PAGE Tris-glycine gels; SP007: Running of polyacrylamide gels; SP008: Running of IEF strips by IPGPhor; SP009: Running of 2D gels; SP010: Running of 2D gels using ZOOM System; SP011: Western blot protocol; SP012: Silver staining; SP013: Coomassie staining; SP014: Quantitating and assesing purity of DNA by UV spec.; SP015: Ligation of DNA fragments with sticky ends; SP016: Ligation of DNA fragments with blunt ends; SP017: Restriction endonuclease digestion of DNA; SP018: Running of agarose gels; SP019: Removal of SDS by paired-ion extraction; SP020: LAL assay; SP021: In-gel digestion of proteins; SP022: Preparation of alditol acetate derivatives; SP023: Operation of Orion 310 pH meter; SP024: Operation of Corning 430 pH meter in C210; SP025: Operation of Waters 600E HPLC system; SP027: Operation of french press; SP028: PCR for detection of clones; SP029: Preparation of electro-competent M. smegmatis; SP030: Percent SDS determination in an aqueous solution; SP031: Operation of the N2 Bath; SP031b: Operation of N2 Bath Meter; SP032: Preparative Thin Layer Chromatography; SP033: Thin Layer Chromatography; SP034: AlamarBlue Testing; SP035: Acid-Fast Staining; SP036: Gram Staining; SP037: Operation of rotovap; SP039: ELISA; SP040: Capture ELISA; SP040b: IL-2 Capture ELISA; SP042: Electroporation of M. smegmatis; SP043: Recombinant Plasmid Map Design-VectorNTI; SP044: Sequence Alignment-VectorNTI ContigExpress; SP045: Operation of the HP 5890A Gas Chromatograph; SP046: NMR of Glycolipids