TB Vaccine Testing and Research Materials Contract

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Production Manuals & SOPs

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Production Manuals:: Large Scale Growth; Subcellular Fractions; Preparation and Fractionation of Cell Wall Proper; Lipoarabinomannan (LAM) and Lipomannan (LM); Antibodies; Microarrays

Individual SOPs:

Media: M001: Preparation of GAS medium; M002: Preparation of GAS medium with tween; M003: Preparation of Sauton's medium; M004: Preparation of Sauton's medium with tween; M005: Preparation of 7H9 medium; M006: Preparation of 7H9 medium with tween; M007: Preparation of 7H9-Dextrose medium; M008: Preparation of 7H9-Dextrose medium with tween; M009: Preparation of 7H11 agar plates; M010: Preparation of 7H11-Dextrose agar plates; M011: Preparation of Nutrient agar plates; M012: Preparation of Complete RPMI medium; M013: Preparation of Mycobactin HR Media and Plates
Reagents: R001: Preparation of 32% Triton; R002: Preparation of 1st fix for gels; R003: Preparation of 2nd fix for gels; R004: Preparation of Coomassie stain reagent; R005: Preparation of Coomassie stain destain; R006: Buffer B for polyacrylamide gels; R007: Buffer D for polyacrylamide gels; R008: Preparation of SDS-PAGE Tris-glycine running buffer; R009: SDS-PAGE 10X Tricine running buffer; R010: Preparation of western blot transfer buffer; R011: Preparation of charring spray; R012: Preparation of α-napthol spray; R013: Preparation of Dittmer-Lester spray; R014: Preparation of TAE Buffer
Native Material Production: PP001: Establishing frozen stocks of M. tb (small scale); PP002: Establishing frozen stocks of M. tb (large scale); PP003: Large scale growth of M. tuberculosis; PP004: Gamma-irradiation of M. tb; PP005: Preparation of CFPs and low MW CFPs; PP006: Preparation of CFPs using 2L Stirred Cell; PP007: Preparation of whole cell lysate; PP008: Preparation of subcellular fractions; PP009: Preparation of genomic DNA; PP010: Preparation of TX-114 proteins; PP011: Preparation of mAGP; PP012: Preparation of arabinagalactan; PP013: Preparation of peptidoglycan; PP014: Preparation of mycolic acids; PP015: Preparation of LAM, LM, and PIM (LLP); PP016: Separation of LLP; PP017: QC of LAM and LM; PP018: Isolation of total lipid; PP019: Purification of Con-A reactive CFPs; PP020: Preparation of Ag85 complex; PP021: Preparation of Ag85 A, B, and C; PP022: Purification of MPT32; PP023: Purification of the 16kDa ACR/HspX; PP024: Purification of 38kDa; PP025: Preparation of SDS soluble cell wall protein; PP026: Preparation of PIM; PP027: Purification of SapM; PP028: Preparation of CFP minus LAM; PP029: Purification of Trehalose Dimycolate (TDM); PP030: Purification of Trehalose Monomycolate (TMM); PP031: Purification of 19kDa; PP032: Purification of Mycobactin; PP033: Growth of M.tb for Mycobactin Production; PP034: Preparation of Sulfolipid-1
Recombinant Material Production: RP001: Generation of Recombinant Clone Frozen Stocks; RP002: Establishing Stocks of Low Copy Plasmids; RP003a: Production of Recombinant Proteins; RP003b: Production of Recombinant Proteins Denaturing Conditions; RP004: Production of ESAT6; RP005: Production of CFP10; RP006: Small- Scale Purification of Plasmid DNA; RP007: Production of rec GlcB; RP007msmeg: Production of rec GlcB from M. smegmatis; RP008: Production of rec Rv1411c; RP010: Production of rec Ag85A; RP011: Production of rec Ag85B; RP012: Production of rec Ag85C; RP014: Production of rec DnaK; RP015: Production of rec GroEL2; RP016: Production of rec Mpt64; RP019: Production of rec BRFB; RP020: Production of rec Mpt51; RP021: Production of rec HspX; RP022: Production of rec Rv2626c; RP023: Production of rec Rv0577; RP025: Production of rec KatG; RP026: Production of rec Rv3763; RP028: Production of GroES
Antibody Production: AB100: Cell Line Maintenance; AB101: IgG Purification; AB102: Antibody QC; AB103: Monoclonal Fusion; AB104: Screening and Subcloning; AB105: Freezing Cell Lines
Standard Procedures: SP003: BCA protein assay; SP004: Lyophilization; SP005: Operation of the savant; SP006: Preparation of SDS-PAGE Tris-glycine gels; SP007: Running of polyacrylamide gels; SP008: Running of IEF strips by IPGPhor; SP009: Running of 2D gels; SP010: Running of 2D gels using ZOOM System; SP011: Western blot protocol; SP012: Silver staining; SP013: Coomassie staining; SP014: Quantitating and assesing purity of DNA by UV spec.; SP015: Ligation of DNA fragments with sticky ends; SP016: Ligation of DNA fragments with blunt ends; SP017: Restriction endonuclease digestion of DNA; SP018: Running of agarose gels; SP019: Removal of SDS by paired-ion extraction; SP020: LAL assay; SP021: In-gel digestion of proteins; SP022: Preparation of alditol acetate derivatives; SP023: Operation of Orion 310 pH meter; SP024: Operation of Corning 430 pH meter in C210; SP025: Operation of Waters 600E HPLC system; SP027: Operation of french press; SP028: PCR for detection of clones; SP029: Preparation of electro-competent M. smegmatis; SP030: Percent SDS determination in an aqueous solution; SP031: Operation of the N2 Bath; SP031b: Operation of N2 Bath Meter; SP032: Preparative Thin Layer Chromatography; SP033: Thin Layer Chromatography; SP034: AlamarBlue Testing; SP035: Acid-Fast Staining; SP036: Gram Staining; SP037: Operation of rotovap; SP039: ELISA; SP040: Capture ELISA; SP040b: IL-2 Capture ELISA; SP042: Electroporation of M. smegmatis; SP043: Recombinant Plasmid Map Design-VectorNTI; SP044: Sequence Alignment-VectorNTI ContigExpress; SP045: Operation of the HP 5890A Gas Chromatograph; SP046: NMR of Glycolipids