Sulfolipid-1

Reagent:
Sulfolipid-1, SL-1

Default Quantity:
0.25 mg

Production system:
Sulfolipid-1 (SL-1) is first purified by extracting H37Rv Mtb cells with 2:1 chloroform/methanol and loading onto a silica gel column. The column is washed with chloroform, then eluted with 5% methanol in chloroform. This fraction, enriched in TDM and SL-1, is loaded onto C18 reverse phase SepPak filters and eluting with 60% chloroform in methanol to remove the TDM, then 25% to elute the SL-1.

Notes:
For most requests, this product will be made on demand due to yield and stability issues. Therefore, a delay should be anticipated.

Sulfolipid appears to be very labile. Dry storage at -80°C is recommended to prevent breakdown. As little as two days storage in solution at 4°C causes SL-I to become very non-polar, as evidenced by running on 2D-TLC.

Return to Lipids and Carbohydrates | Return to Materials Available