Recombinant Plasmids

Reagent:
Recombinant expression vectors containing Mycobacterium tuberculosis genes and protocols for generation of recombinant proteins.

Default Quantity:
1 µg plasmid DNA

Production system:
All genes are amplified by PCR and cloned into pET23b or pET15b (Novagen) for expression in E. coli or pVV16 (a derivative of pMV261 allowing His-tag fusion) for expression in M. smegmatis. All proteins are His-tagged.

Notes:
Beginning October 1, 2005 recombinant proteins will only be provided as reference standards or for large scale studies (see below for more details). All other laboratories will receive plasmid DNA and detailed protocols for gene expression and protein purification.

Plasmids listed are expressed in E. coli unless otherwise stated. In general, plasmids expressed in E. coli are cloned without a signal sequence and those expressed in M. smegmatis are cloned with signal sequence (unless otherwise noted).

Reference Standards | Large Scale Studies

* Approximate yields are given if known, based on recovery of purified protein from a 1L culture.

Note: This list is updated on a continual basis as new recombinant products are generated.

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