Phosphatidylinositol Mannosides 1 & 2
Reagent:
Phosphatidylinositol mannoside, PIM1,2
Default Quantity:
0.5 mg
Production system:
Cells are extracted with either chloroform/methanol/water (10:10:3). The organic soluble fraction is dried and titrated with cold acetone. The acetone insoluble fraction is applied to preparative thin-layer chromatography plates (20cm, Silica gel 60 F254, 1mm) in a solvent system of chloroform/methanol/water (60:30:6). PIMs are extracted from the silica plates and released from the dried matrix using 40% methanol in chloroform.
Notes:
No structural differences between the PIMs of M. tuberculosis and M. bovis are known.
References:
Brennan, P., and Ballou, C.E., J. Biol. Chem. 242:3046, 1967.
Khoo, K.-H., et al. Glycobiol. 5:117, 1995.
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