Culture Filtrate Proteins

Reagent:
Mixed Culture Filtrate Proteins, CFP

Default Quantity:
1 mg

Production system:
Each strain is grown to late-log phase (day 14) in glycerol-alanine-salts (GAS) medium. The culture supernatant is harvested from the live cells by passing through a 0.2 micron filter. The CFP is concentrated by Amicon ultrafiltration using a membrane with a molecular weight cutoff of 5,000 Da. The concentrated material is dialyzed against 0.01 M ammonium bicarbonate, quantitated with the BCA protein assay, aliquoted, lyophilized, and stored at -80°C.

Notes:
This preparation includes most of the excreted/secreted proteins of M. tuberculosis. Individual lots are subjected to quality control procedures to ensure uniformity and lack of bacterial contamination.

References:
Dobos, K.M. et al., Infect. Immun. 63:2846, 1995.
Sonnenberg, M.G. et al., Infect. Immun. 65:4515, 1997.

Return to Subcellular Fractions | Return to Materials Available