Inactivated Mycobacterium tuberculosis Whole Cells

Reagent:
Inactivated Mycobacterium tuberculosis Whole Cells, γ-irradiated whole cells

Default Quantity:
10 grams

Strains Available:
H37Rv, CDC1551, and HN878

Production system:
Each strain is grown to late-log phase (day 14) in glycerol-alanine-salts (GAS) medium. The bacilli are harvested by filtration washed with PBS pH 7.4, pelleted by centrifugation and frozen at -80°C. For inactivation, the cell pellet is exposed to 2.4 megaRads of ionizing gamma irradiation using a ¹³⁷Cs source. Confirmation of inactivation is performed by Alamar Blue Assay. The inactivated cell pellets are stored at -80°C.

Notes:
A dose of 2.4 mRads of gamma irradiation kills M. tuberculosis to a 10²⁰ degree of certainty while maintaining 93-95% of the biological activity of the enzymes.

Return to Subcellular Fractions | Return to Materials Available