Antigen 85 Complex

Reagent:
Antigen 85 Complex, Ag85, Mixture of Rv3804c (Ag85A, FbpA), Rv1886c (Ag85B, FbpB), and Rv0129c (Ag85C, FbpC)

Default Quantity:
500 µg

Production system:
CFPs are precipitated with a 40% saturated solution of ammonium sulfate. The precipitate is suspended and applied to phenyl sepharose HPLC. Antigen 85 is obtained by increasing the pH and eluting with a high concentration of ethylene glycol. If necessary, additional purification is performed using size exclusion chromatography. The purified product is dialyzed against 0.01 M ammonium bicarbonate, lyophilized, and stored at -80°C.

Notes:
The Ag85 complex contains three gene products of 30-31 kDa. The individual components of the Ag85 complex (Ag85A, Ag85B and Ag85C) are available for specific research needs, and the default quantity for these is 250 µg.

References:
Wiker H.G. and Harboe M., Microbiol. Rev. 56:648, 1992.

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