Diane J. Ordway
Assistant Professor

Phone: 491-7469
Fax: 491-1815
Email: D.Ordway-Rodriguez@Colostate.Edu
Office: B320 Microbiology Building
Lab: C312 Microbiology Building

 

Degrees

• B.Sc., Colorado State University, USA
• Ph.D., London School of Hygiene & Tropical Medicine, England

Research Interests

Immunology, Vaccines and Drugs against Pathogenic Mycobacteria

• Worldwide the rate of drug-sensitive, multi-drug and extensively drug resistant Mycobacterium tuberculosis cases are increasing. The only available vaccine against M. tuberculosis, M. bovis Bacillus Calmette-Guérin (BCG) is unreliable and the use of anti-mycobacterial drugs has only lead to high rates of drug resistance. Primarily I am interested in elucidating the T cell and macrophage/dendritic cellular immune responses against Mycobacterium tuberculosis through the use of novel multi-parameter flow cytometry in murine and guinea pigs models. These animal models are used to test vaccines and drugs and provide us with information on the nature of induction of protective immunity.
• In addition, my interests are to further understand whether drug resistant strains and drug sensitive strains respond differently to the innate and adaptive host immune response.
• I also collaborate with scientists in clinical research focused on elucidation of the immune mechanisms against Mycobacterium abscessus, Mycobacterium avium and Mycobacterium paratuberculosis.

Selected Publications

Pub Med for Ordway D.

• Ordway, D., M. G. Sonnenberg, S. A. Donahaue, J. T. Belisle and I. M. Orme. Drug resistance strains of Mycobacterium tuberculosis exhibit a range of virulence for mice. Infection and Immunity 1995. 63: 741-43.
• Ordway, D., S. K. Furney, P. J. Brennan, J. T. Belisle and I. M. Orme. Characteristics of protective immunity engendered by vaccination of mice with purified culture filtrate protein antigens of Mycobacterium tuberculosis. Immunology 1995. 85: 502-8.
• Ordway, D., Viveiros, M., Leandro, C., and L. Amaral. Chlorpromazine and thioridazine have intracellular killing activity against phagocytosed multi-drug resistant Mycobacterium tuberculosis. Antimicrobial Agents and Chemotherapy 2003. 47:917-922.
• Kristiansen MM, C. Leandro, D. Ordway, M. Martins, M. Viveiros, T. Pacheco, JE Kristiansen and L. Amaral. Phenothiazines alter resistance of methicillin-resistant strains of Staphylococcus aureus (MRSA) to oxacillin in vitro. International Journal of Antimicrobial Agents. 2003. 22: 250-53.
• Ordway, D., Costa, L., Martins, M., Silveira, H., Amaral, L. , Arroz, M.J., Ventura, F.A. and Dockrell, H. Increased IL-4 responses to virulent Mycobacterium tuberculosis in health care workers, who went on to present with primary TB Journal of Infectious Disease 2004. 190:756-766.
• Ordway, D., L. Pinto, L.Costa, M. Martins, C. Leandro, M. Viveiros, L. Amaral, M. J. Arroz, F. A. Ventura, and H. M. Dockrell. Gamma delta T cell responses associated with the development of Tuberculosis in health care workers. FEMS Immunology & Medical Microbiology 2005. 43:3339-3351.
• Ordway, D., M. Henao-Tamayo, I. M. Orme, and M. Gonzalez-Juarrero. Foamy macrophages within lung granulomas of mice infected with Mycobacterium tuberculosis express molecules characteristic of dendritic cells and express anti-apoptotic markers of the TRAF family. 2005. Journal of Immunology 175:3873-3881.
• Park, J.S., M. Henao-Tamayo, M. Gonzalez-Juarrero, I.M. Orme, D. Ordway. Virulent clinical isolates of Mycobacterium tuberculosis grow rapidly and induce cellular necrosis in murine macrophages. 2006. Journal of Leukocyte Biology 79:1-7.
• Ordway, D., M. Harton, M. Henao-Tamayo, I.M. Orme, and M. Gonzalez-Juarrero. Enhanced macrophage activity in granulomatous lesions of immune mice challenged with Mycobacterium tuberculosis. 2006. Journal of Immunology  176:4931-4939.
• Ordway, D., M. Henao-Tamayo, M. Harton, G. Palanisamy, J. Troudt, C. Shanley, R. J. Basaraba, and I. M. Orme. The hypervirulent Mycobacterium tuberculosis strain HN878 induces a potent TH1 response followed by rapid down-regulation. 2007. Journal of Immunology 179:522-31.
• Ordway, D., G. Palanisamy, M. Henao-Tamayo, E. Smith, C. Shanley, I. M. Orme and R. J. Basaraba. Cellular immune responses during Mycobacterium tuberculosis infection in the guinea pig. 2007. Journal of Immunology 179:2532-41.
• Ordway, D., Higgins D. M., Sanchez-Campillo J., Spencer J., Henao-Tamayo M. , Harton M. , Orme I., and M. Gonzalez-Juarrero. 2007. XCL1 (lymphotactin) chemokine produced by activated CD8 T cells during the chronic stage of infection with Mycobacterium tuberculosis negatively affects production of IFN-γ by CD4 T cells and participates in granuloma stability. 2007. Journal of Leukocyte Biology 82:1221-9.