Mary Jackson
Assistant Professor
Phone: 491-3582
Fax: 491-1815
Email:
Mary.Jackson@Colostate.Edu
Office: B222 Microbiology Building
Lab: B203-B204, Microbiology Building
Degrees
M.Sc. - Ecole Nationale Supérieure Agronomique de Rennes, France.
Ph. D. - Institut Pasteur, Paris; Ecole Nationale Supérieure Agronomique de Rennes, France
Research Interests
Our research focuses on the biosynthetic pathways (enzymes and transporters) of major cell envelope components - (lipo)polysaccharides, (glyco)lipids, and fatty acids - playing important roles in the physiology and/or pathogenicity of Mycobacterium tuberculosis. Another aspect of our research concerns the mechanisms of resistance of slow- and fast-growing mycobacteria of clinical interest to drugs and disinfectants.
In addition to providing fundamental knowledge about the biochemistry of M. tuberculosis and other mycobacteria, the broad goals of our work are i) to determine the contribution of various cell envelope components to pathogenicity, ii) to identify novel anti-tuberculosis drug targets and, iii), to generate novel attenuated mycobacterial strains as candidate live vaccines against tuberculosis.
The Lab (2009)
Selected Publications
2009
Kaur, D., Pham, H., Larrouy-Maumus, G., Rivière, M., Vissa, V., Guerin, M. E., Puzo, G., Brennan, P. J. and Jackson, M. (2009) Initiation of methylglucose lipopolysaccharide biosynthesis in mycobacteria. PLoS ONE. 4(5): e5447. doi:10.1371/journal.pone.0005447.
Santangelo, M. P., L. Klepp, J. Nuñez-García, F. Blanco, M. Soria, M. del Carmen García-Pelayo, M. V. Bianco, A. Cataldi, P. Golby, M. Jackson, S. V. Gordon and F. Bigi. (2009) Mce3R, a TetR-type transcriptional repressor, controls the expression of a regulon involved in lipid metabolism in Mycobacterium tuberculosis. Microbiology. 155: 2245-2255.
Guerin, M. E., Schaeffer, F., Chaffotte, A., Gest, P., Giganti, D., Korduláková, J., van der Woerd, M., Jackson, M. and Alzari, P. M. (2009) Substrate-induced conformational changes in the essential peripheral membrane-associated mannosyltransferase PimA from Mycobacteria. Implications for catalysis. J Biol Chem 284 : 21613-21625.
Svetlíková, Z., H. Škovierová, M. Niederweis, J.-L. Gaillard, G. McDonnell and Jackson, M. (2009) The role of porins in the susceptibility of Mycobacterium smegmatis and Mycobacterium chelonae to aldehyde-based disinfectants and drugs. Antimicrob Agents Chemother 53: 4015-4018.
Škovierová, H., G. Larrouy-Maumus, J. Zhang, D. Kaur, N. Barilone, J. Korduláková, M. Gilleron, S. Guadagnini, M. Belanová, M.-C. Prevost, B. Gicquel, G. Puzo, D. Chatterjee, P. J. Brennan, J. Nigou, and Jackson, M. (2009) AftD, a novel essential arabinofuranosyltransferase from mycobacteria. Glycobiology (in press)
Guerin, M. E., D. Kaur, B. S. Somashekar, S. Gibbs, P. Gest, D. Chatterjee, P. J. Brennan, and Jackson, M. (2009) New insights into the early steps of phosphatidylinositol mannosides biosynthesis in mycobacteria. PimB' is an essential enzyme of Mycobacterium smegmatis. J. Biol. Chem. (in press)
Scherman, H., D. Kaur, H. Pham, H. Škovierová, M. Jackson, and P. J. Brennan. (2009) Identification of a polyprenylphosphomannosyl synthase involved in the synthesis of mycobacterial mannosides. J. Bacteriol. (in press)
Kaur, D., Guerin, M. E., Škovierová, H., Brennan, P. J. and Jackson, M. (2009) Biogenesis of the cell wall and other glycoconjugates of Mycobacterium tuberculosis. Advances in Applied Microbiology. 69: pp.23-78.
Jackson, M., and Brennan, P.J. (2009) Polymethylated polysaccharides from Mycobacterium species revisited. J. Biol. Chem. 284: pp. 1949-1953.
2008
Chesne-Seck, M.-L., N. Barilone, F. Boudou, J. Gonzalo Asensio, P. E. Kolattukudy, C. Martin, S. T. Cole, B. Gicquel, D. N. Gopaul, and Jackson, M. (2008). A point mutation in the twoChesne-Seck, M.-L., N. Barilone, F. Boudou, J. Gonzalo Asensio, P. E.
Kolattukudy, C. Martin, S. T. Cole, B. Gicquel, D. N. Gopaul, and Jackson, M.
(2008). A point mutation in the two-component regulator PhoP-PhoR accounts for
the absence of polyketide-derived acyltrehaloses but not that of phthiocerol
dimycocerosates in Mycobacterium tuberculosis H37Ra J. Bact.
190, pp. 1329-1334.
Pitarque, S., Larrouy-Maumus, G., Payré, B., Jackson, M., Puzo, G., and Nigou, J.
(2008) The immunomodulatory lipoglycans, lipoarabinomannan and lipomannan, are
exposed at the mycobacterial cell surface. Tuberculosis 88, pp. 560-565. 1.
Liav, A., Angala, S.K., Brennan,
P.J., and Jackson, M. (2008) N-D-Aldopentofuranosyl-N’-[p-(isoamyloxy)phenyl]-thiourea
derivatives: Potential anti-TB therapeutic agents. Bioorg. Med. Chem. Lett.
18: 2649-2651.
Fonvielle, M., Coincon, M., Daher, R., Desbenoit, N., Kosieradzka, K., Barilone, N., Gicquel, B., Sygusch, J., Jackson, M., and Therisod, M. (2008) Synthesis and biochemical evaluation of selective inhibitors of class II fructose bis-phosphate aldolases: towards new synthetic antibiotics.
Chem. Eur. J. 14: 8521-8529.
Sambou, T., Dinadayala, P., Stadthagen, G., Barilone, N., Bordat, Y., Constant, P., Levillain, F., Neyrolles, O., Gicquel, B., Lemassu, A., Daffé, M., and Jackson, M. (2008) Capsular glucan and intracellular glycogen of
Mycobacterium tuberculosis: Biosynthesis and impact on the persistence in mice.
Mol. Microbiol. 70: 762-774.
Kaur, D., Obregón-Henao, A., Pham, H., Chatterjee, D., Brennan, P.J., and Jackson, M. (2008) Lipoarabinomannan of
Mycobacterium; mannose capping by a multifunctional terminal mannosyltransferase.
Proc. Natl. Acad. Sci. USA 105: 17973-17977.
Gest, P., Kaur, D., Pham, H.T., van der Woerd, M., Hansen, E., Brennan, P.J., Jackson, M., and Guerin, M.E. (2008) Preliminary crystallographic analysis of GpgS, a key glucosyltransferase involved in methylglucose lipopolysaccharide biosynthesis in
Mycobacterium tuberculosis. Acta Crystallogr Sect F Struct Biol Cryst Commun
F64: 1121–1124.
Gilleron, M., Jackson, M., Nigou, J., and Puzo, G. (2008) Structure, activities and biosynthesis of the Phosphatidyl-myo-Inositol-based lipoglycans In The Mycobacterial Cell Envelope: an overview. Editors: M. Daffé & J.-M. Reyrat; ASM Press, Washington DC. Chap. 6, 75-105.
2007
Guerin, M.E., Korduláková, J., Schaeffer, F., Svetlikova, Z., Buschiazzo, A., Giganti, D., Gicquel, B., Mikusová, K., Jackson, M., and Alzari, P.M. (2007) Molecular recognition and interfacial catalysis by the essential phosphatidylinositol mannosyltransferase PimA from mycobacteria.
J. Biol. Chem 282: 20705-20714.
Stadthagen, G., Sambou, T., Guerin, M., Barilone, N., Boudou, F., Korduláková, J., Charles, P., Alzari, P.M., Lemassu, A., Daffé, M., Puzo, G., Gicquel, B., Rivière, M., and Jackson, M. (2007) Genetic basis for the biosynthesis of methylglucose lipopolysaccharides in
Mycobacterium tuberculosis. J. Biol. Chem. 282: 27270-27276.
Kaur, D., M. R. McNeil, K.-H. Khoo, D. Chatterjee, D. C. Crick, M. Jackson, and P. J. Brennan. (2007) New insights into the biosynthesis of mycobacterial lipomannan arising from deletion of a conserved gene.
J. Biol. Chem 282:27133-27140.
Korduláková, J., Y. L. Janin, A. Liav, N. Barilone, T. Dos Vultos, J. Rauzier, P. J. Brennan, B. Gicquel, and M. Jackson. (2007) Isoxyl activation by EthA is required for bacteriostatic activity against
Mycobacterium tuberculosis. Antimicrob. Agents Chemother. 51:3824-3829.
Jackson, M., Stadthagen, G., and Gicquel, B. (2007) Long-chain multiple methyl-branched fatty acid-containing lipids of
Mycobacterium tuberculosis: biosynthesis, transport, regulation and biological activities.
Tuberculosis 87 : 78-86.
Berg, S., D. Kaur, M. Jackson, and P. J. Brennan. (2007) The glycosyltransferases of
Mycobacterium tuberculosis- roles in the synthesis of arabinogalactan, lipoarabinomannan, and other glycoconjugates.
Glycobiology 17:35R-56R.
Marsollier, L., P. Brodin, M. Jackson, J. Korduláková, P. Tafelmeyer, E. Carbonnelle, J. Aubry, G. Milon, P. Legras, J.-P. Saint-Andre, C. Leroy, J. Cottin, M.-L. Joly Guillou, G. Reysset, and S. T. Cole. (2007) Impact of
Mycobacterium ulcerans biofilm on tranmissibility to ecological niches and Buruli ulcer pathogenesis.
PLoS Pathogens 3:e62.
Other selected publications
Kaur, D., Berg, S., Dinadayala, P., Gicquel, B., Chatterjee, D., McNeil, M.R., Vissa, V., Crick, D.C., Jackson, M., and Brennan, P.J. (2006) Biosynthesis of mycobacterial lipoarabinomannan: Role of a branching mannosyltransferase.
Proc. Natl. Acad. Sci. USA 103: 13664-13669.
Sulzenbacher, G., Canaan, S., Bordat, Y., Neyrolles, O., Stadthagen, G., Roig-Zamboni, V., Rauzier, J., Maurin, D., Laval, F., Daffé, M., Cambillau, C., Gicquel, B., Bourne, Y., and Jackson, M. (2006) LppX is a lipoprotein required for the translocation of phthiocerol dimycocerosates to the surface of
Mycobacterium tuberculosis. EMBO J. 25 : 1436-1444.
Gonzalo Asensio, J., Maia, C., Ferrer, N.L., Barilone, N., Laval, F., Soto, C., Winter, N., Daffé, M., Gicquel, B., Martin, C., and Jackson, M. (2006) The virulence-associated two-component PhoP-PhoR system controls the biosynthesis of polyketide-derived lipids in
Mycobacterium tuberculosis. J. Biol. Chem. 281: 1313-1316.
Rousseau, C., Winter, N., Pivert, E., Bordat, Y., Neyrolles, O., Avé, P., Huerre, M., Gicquel, B. and Jackson, M. (2004) Production of phthiocerol dimycocerosates protects
Mycobacterium tuberculosis from the cidal activity of reactive nitrogen intermediates produced by macrophages and modulates the early immune response to infection.
Cell. Microbiol., 6, 277-287.
Korduláková, J., Gilleron, M., Puzo, G., Brennan, P. J., Gicquel, B., Mikusová, K. and Jackson, M. (2003) Identification of the required acyltransferase step in the biosynthesis of the phosphatidylinositol mannosides of
Mycobacterium species. J. Biol. Chem., 278, 36285-36295.
Korduláková, J., M. Gilleron, K. Mikusová, G. Puzo, P. J. Brennan, B. Gicquel, and M. Jackson (2002) Definition of the first mannosylation step in phosphatidylinositol synthesis: PimA is essential for growth of mycobacteria.
J. Biol. Chem. 277, 31335-31344.
Raynaud, C., C. Guilhot, J. Rauzier, Y. Bordat, V. Pelicic, R. Manganelli, I. Smith, B. Gicquel, and M. Jackson (2002) Phospholipases C are involved in the virulence of
Mycobacterium tuberculosis. Mol. Microbiol. 45, 203-217.
Jackson, M., Crick, D. C. and P. J. Brennan (2000) Phosphatidylinositol is an essential phospholipid of mycobacteria.
J. Biol. Chem., 275, 30092-30099.
Jackson, M., Phalen, S. W., Lagranderie, M., Ensergueix, D., Chavarot, P., Marchal, G., McMurray, D. N., Gicquel, B. and C. Guilhot (1999) Persistence and protective efficacy of a
Mycobacterium tuberculosis auxotroph vaccine. Infect. Immun., 67, 2867-2873.
Jackson, M., Raynaud, C., Lanéelle, M.-A., Guilhot, C., Laurent-Winter, C., Ensergueix, D., Gicquel B. and M. Daffé (1999) Inactivation of the antigen 85C gene profoundly affects the mycolate content and alters the permeability of the
Mycobacterium tuberculosis cell envelope. Mol. Microbiol., 31, 1573-1587.
Pelicic, V., Jackson, M., Reyrat, J.-M., Jacobs Jr., W. R., Gicquel, B. and C. Guilhot (1997) Efficient allelic exchange and transposon mutagenesis in
Mycobacterium tuberculosis. Proc. Natl. Acad. Sci USA, 94, 10955-10960.
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