Vol. 14 | No. 3
2017 Zoetis Distinguished Veterinary Teacher Award
Dr. Christine Olver, Associate Professor of Clinical Pathology, was selected by our veterinary graduates to receive the 2017 Zoetis Distinguished Veterinary Teacher Award. Dr. Olver received the honor at the DVM Hooding Ceremony.
Josh has a recognizable attribute that is key to discovery: an inquisitive approach to what he has yet to understand. To augment his classroom work, Josh naturally joined Delphi Chatterjee's research group early in his career (Fall 2015). He subsequently received a MIP Undergraduate Research Fellowship, providing him with funding to pursue an independent research project in the lab. Joshua is a very committed student, not averse to spending evening hours in the lab in pursuit of finishing what he started to learn something new. Beyond the scope of Joshua’s intellectual capabilities and engagement in science, he exudes a sense of congeniality in the workplace. He is mature and positive, takes responsibility for his decisions and is a source of humor in a variety of situations. There is little doubt that Josh will be a valuable contributor to future research and/or medical settings.
CONGRATULATIONS JOSH !
Congratulations to Savanah for being chosen as an outstanding graduate representing CVMBS for CSU. Savannah was a sophomore in high school in Crawford, Colorado, when her father was diagnosed with cancer. Doctors didn’t know if he would make it to her graduation, and she felt helpless. At the time, she was learning about proteins and cancer in science class. She realized her calling: to figure out what’s behind diseases like cancer and to prevent other families from feeling disheartened. We are pleased to report that her father’s cancer has been in remission for six years and that Savanah will start work on a PhD at CSU in the Fall. Her goal is to be a professor one day.
MIP Graduate Students who received their Degrees on May 12th...
Emily Blizzard- PhD
MIP Undergraduate Students who received their B.S. Degrees on May 13th...
Dr. Santangelo Recognized at the 2017 World OA Congress
Kelly Santangelo recently presented a plenary talk as the “Top Rated Abstract” at the 2017 World Congress on Osteoarthritis held by the Osteoarthritis Research Society International (OARSI). The title of her talk was “Systemic antagonism of Toll-like Receptor 4 reduces post-traumatic osteoarthritis in a murine model”.
MIP Residents Pass ACVP Phase I Board Exam
CONGRATULATIONS to Alex Byas and Lauren Harris (pictured below) on passing the first phase of the ACVP board exam.
And the Spot Award Goes To...
Congratulations to Corrine and Madelein for winning a CVMBS SPOT award!
MIP Shines in CURC 2017
MIP Micro Majors Jordanne Lesher and Delaney Thompson both received Best in Show recognitions at CURC 2017. Jordanne, pictured above, received the award for her poster “Identification of Small Molecules that Modulate Cyclic di-GMP Signaling and Biofilm Formation in Pseudomonas aeruginosa”. Her mentors were Grace and Brad Borlee. Delaney Thompson worked in the BMS Dept with scientist Sean Hammond.
Overall, MIP had a fantastic showing at the CURC. 32 students (14% of our majors) presented posters, not counting 4 non-MIP student participants who were mentored by MIP faculty. MIP majors took home 16.5% of the science awards given out at the CURC - as we have only 0.8% of CSU students this is particularly amazing!!! Students from the newly minted Microbiology Undergraduate Research Fellowship Program received 6 awards at the symposium.
Here’s a list of MIP-associated awardees and their mentors:
Best in Show: Delaney Thompson, Mentor: Sean Hammond
Best in Show: Jordanne Lesher, Mentor: Brad Borlee
Highest Honors: Roslyn Dermody, Mentor: Delphi Chatterjee.
Highest Honors: Joshua Daum, Mentor: Delphi Chatterjee
Highest Honor: Erica Reith, Mentor: Carol Wilusz
High Honor: Erin Markle, Mentor: Brian Foy
High Honor: Molly Price, Mentor: Ramesh Akkina
High Honor: Robin Blackwood, Mentor: Thomas Santangelo
High Honor: Elizabeth Gordon, Mentor: Sarah Kane
High Honor: Jordan Powers, Mentor: Sue VandeWoude
College Honors: Regina Argo, Mentor: Julie Monreno
College Honors: Joseph Gallegos, Mentor: Bian Jifeng
College Honors: Jessica Gaevert, Mentor: Karen Dobos
College Honors: Ariel Timkovich, Mentor: Kelly Santangelo
Finally, a special thanks to everyone who volunteered to judge the posters.
Congratulations to MIPers who won CVMBS Living Our Values Awards this Spring
Team – Group Award
Congratulations to all!
MIP Students Shine at the Multicultural Undergraduate Research Art and Leadership Conference (MURALS)
Jordan Aragon (Mentor Alan Schenkel), Elizabeth Gordon (Mentor Mark Zabel), Tavo Ontiveros (Mentors Jeff and Carol Wilusz), and Savannah Rocha (mentor Mark Zabel)
See all the photos here.
4 MIP students receive GAUSSI Fellowships
Congratulations to Shaun Cross (Stenglein lab), Bridget Eklund (Abdo lab), Mike Mangalea (UNC fan in the Borlee lab) and Jessie Filer (Geiss lab) for receiving a one year fellowship from the GAUSSI Program. The NSF-sponsored GAUSSI fellowship supports their tuition and stipend for one year and allows these students to substantially increase their bioinformatics capability through targeted coursework.
Congratulations to all!
In The News...
Rovnak-led Team Finds an Efficient Way to Sniff Out Zika Virus
Joel and a cast of characters from MIP/AIDL made a big splash this month by publishing a high impact study in Science Translational Medicine describing an isothermal LAMP assay to rapidly, easily and cheaply detect and distinguish Zika virus from other arboviruses in samples. Numerous news sources picked up on the report, including the Scientist, the Denver Post, and of course CSU Source. Check out the article The Scientist write up and the CSU Source overview here.
Mosquitoes transmit unique West Nile Virus populations during each feeding episode
The concept of novel infectious diseases emerging is just plain scary. OK, maybe not as scary as the trailer to the ‘IT’ movie, but still pretty unsettling. As we approach the start of another mosquito season here in the Fort, it would sure be nice if we better understood how more virulent strains of a pathogen like West Nile virus could emerge from these winged vectors to terrorize our humble community. Thanks to the able Ebel Team from the AIDL, we now have some more clues to the dynamics of West Nile virus evolution as they are transmitted between mosquitoes and reservoir species.
What Nathan et al did was pretty straightforward but a heckuva lot of work. First they took two species of Culex mosquitoes, infected them with West Nile Virus, and fully sequenced the viruses present in mosquito saliva 8, 14 and 18 days later. As an aside, you should also appreciate that figuring out a way to non-invasively collect mosquito spit isn’t trivial (kinda like a MIPnews writer trying to figure out a way of getting real laughs from a reader instead of just pun-induced smirks…). What they found was that C. tarsalis had more virus in its spit than C. quinquefasciatus and – much more importantly – that the diversity of the viruses found in the spit was extremely high. In fact, there were even substantially different viruses appearing over time in saliva samples from the same mosquito! This clearly establishes that West Nile virus variants that are generated naturally from error-prone RNA replication can accumulate to rather high levels under low selective pressure when they arise in the mosquito vector. Next, they let the skeeters feed on young chickens and checked out the sequences of the viruses present in bird serum. Surprisingly, most of the variants in thevirus population that were present in the skeeter saliva now disappeared as growth in the bird provided strong selective pressure and allowed populations of only very fit viruses to expand. When these chicken-selected viruses were passed on back to a new mosquito via a blood meal, most of the skeeters that fed on the birds died. In summary, the genetic drift of West Nile virus is clearly extremely high in mosquitoes – but there is a large selective pressure in vertebrate hosts that removes a lot of variants from the virus population. These cycles of virus diversification and purifying selection make it clearer how novel virus variants can emerge in nature and wreak havoc on human health.
MIP Publications May 2017
Charlins P, Schmitt K, Remling-Mulder L, Hogan LE, Hanhauser E, Hobbs KS, Hecht F, Deeks SG, Henrich TJ, Akkina R. A humanized mouse-based HIV-1 viral outgrowth assay with higher sensitivity than in vitro qVOA in detecting latently infected cells from individuals on ART with undetectable viral loads. Virology. 2017 Apr 19;507:135-139.
Ronacher K, van Crevel R, Critchley J, Bremer AA, Schlesinger LS, Kapur A, Basaraba R, Kornfeld H, Restrepo BI. Defining a research agenda to address the converging epidemics of tuberculosis and diabetes. Part 2: Underlying biological mechanisms. Chest. 2017 Apr 19. pii: S0012-3692(17)30725-0. doi: 10.1016/j.chest.2017.02.032.
Critchley JA, Restrepo BI, Ronacher K, Kapur A, Bremer AA, Schlesinger LS, Basaraba R, Kornfeld H, van Crevel R. Defining a research agenda to address the converging epidemics of tuberculosis and diabetes. Part 1: Epidemiology and clinical management. Chest. 2017 Apr 19. pii: S0012-3692(17)30730-4. doi: 10.1016/j.chest.2017.04.155.
Sharpe SA, White AD, Sibley L, Gleeson F, Hall GA, Basaraba RJ, McIntyre A, Clark SO, Gooch K, Marsh PD, Williams A, Dennis MJ. An aerosol challenge model of tuberculosis in Mauritian cynomolgus macaques. PLoS One. 2017 Mar 8;12(3):e0171906. doi: 10.1371/journal.pone.0171906.
Nguyen TV, Blackledge MS, Lindsey EA, Minrovic BM, Ackart DF, Jeon AB, Obregón-Henao A, Melander RJ, Basaraba RJ, Melander C. The Discovery of 2-Aminobenzimidazoles That Sensitize Mycobacterium smegmatis and M. tuberculosis to β-Lactam Antibiotics in a Pattern Distinct from β-Lactamase Inhibitors. Angew Chem Int Ed Engl. 2017 Mar 27;56(14):3940-3944.
Crawford JE, Alves JM, Palmer WJ, Day JP, Sylla M, Ramasamy R, Surendran SN, Black WC 4th, Pain A, Jiggins FM. Population genomics reveals that an anthropophilic population of Aedes aegypti mosquitoes in West Africa recently gave rise to American and Asian populations of this major disease vector. BMC Biol. 2017 Feb 28;15(1):16. doi: 10.1186/s12915-017-0351-0.
Van Hoeven N, Fox CB, Granger B, Evers T, Joshi SW, Nana GI, Evans SC, Lin S, Liang H, Liang L, Nakajima R, Felgner PL, Bowen RA, Marlenee N, Hartwig A, Baldwin SL, Coler RN, Tomai M, Elvecrog J, Reed SG, Carter D. Formulated TLR7/8 Agonist is a Flexible, Highly Potent and Effective Adjuvant for Pandemic Influenza Vaccines. Sci Rep. 2017 Apr 21;7:46426. doi: 10.1038/srep46426.
Amarasinghe GK, Bào Y, Basler CF, Bavari S, Beer M, Bejerman N, Blasdell KR, Bochnowski A, Briese T, Bukreyev A, Calisher CH, Chandran K, Collins PL, Dietzgen RG, Dolnik O, Dürrwald R, Dye JM, Easton AJ, Ebihara H, Fang Q, Formenty P, Fouchier RA, Ghedin E, Harding RM, Hewson R, Higgins CM, Hong J, Horie M, James AP, Jiāng D, Kobinger GP, Kondo H, Kurath G, Lamb RA, Lee B, Leroy EM, Li M, Maisner A, Mühlberger E, Netesov SV, Nowotny N, Patterson JL, Payne SL, Paweska JT, Pearson MN, Randall RE, Revill PA, Rima BK, Rota P, Rubbenstroth D, Schwemmle M, Smither SJ, Song Q, Stone DM, Takada A, Terregino C, Tesh RB, Tomonaga K, Tordo N, Towner JS, Vasilakis N, Volchkov VE, Wahl-Jensen V, Walker PJ, Wang B, Wang D, Wang F, Wang LF, Werren JH, Whitfield AE, Yan Z, Ye G, Kuhn JH. Taxonomy of the order Mononegavirales: update 2017. Arch Virol. 2017 Apr 7. doi: 10.1007/s00705-017-3311-7.
Tran AT, Watson EE, Pujari V, Conroy T, Dowman LJ, Giltrap AM, Pang A, Wong WR, Linington RG, Mahapatra S, Saunders J, Charman SA, West NP, Bugg TD, Tod J, Dowson CG, Roper DI, Crick DC, Britton WJ, Payne RJ. Sansanmycin natural product analogues as potent and selective anti-mycobacterials that inhibit lipid I biosynthesis. Nat Commun. 2017 Mar 1;8:14414. doi: 10.1038/ncomms14414.
Grubaugh ND, Fauver JR, Rückert C, Weger-Lucarelli J, Garcia-Luna S, Murrieta RA, Gendernalik A, Smith DR, Brackney DE, Ebel GD. Mosquitoes Transmit Unique West Nile Virus Populations during Each Feeding Episode. Cell Rep. 2017 Apr 25;19(4):709-718.
Hien AS, Sangaré I, Coulibaly S, Namountougou M, Paré-Toé L, Ouédraogo AG, Diabaté A, Foy BD, Dabiré RK. Parasitological Indices of Malaria Transmission in Children under Fifteen Years in Two Ecoepidemiological Zones in Southwestern Burkina Faso. J Trop Med. 2017;2017:1507829. doi: 10.1155/2017/1507829. Epub 2017 Feb 13.
Hoover CE, Davenport KA, Henderson DM, Denkers ND, Mathiason CK, Soto C, Zabel MD, Hoover EA. Pathways of Prion Spread during Early Chronic Wasting Disease in Deer. J Virol. 2017 Mar 1. pii: JVI.00077-17. doi: 10.1128/JVI.00077-17.
Mayo C, Lee J, Kopanke J, MacLachlan NJ. A review of potential bluetongue virus vaccine strategies. Vet Microbiol. 2017 Mar 18. pii: S0378-1135(17)30324-3. doi: 10.1016/j.vetmic.2017.03.015.
Shikiya RA, Langenfeld KA, Eckland TE, Trinh J, Holec SA, Mathiason CK, Kincaid AE, Bartz JC. PrPSc formation and clearance as determinants of prion tropism. PLoS Pathog. 2017 Mar 29;13(3):e1006298. doi: 10.1371/journal.ppat.1006298.
Radakovich LB, Truelove MP, Pannone SC, Olver CS, Santangelo KS. Clinically healthy overweight and obese dogs differ from lean controls in select CBC and serum biochemistry values. Vet Clin Pathol. 2017 Mar 8. doi: 10.1111/vcp.12468.
Bernut A, Herrmann JL, Ordway D, Kremer L. The Diverse Cellular and Animal Models to Decipher the Physiopathological Traits of Mycobacterium abscessus Infection. Front Cell Infect Microbiol. 2017 Apr 4;7:100. doi: 10.3389/fcimb.2017.00100.
Thanna S, Goins CM, Knudson SE, Slayden RA, Ronning DR, Sucheck SJ. Thermal and Photoinduced Copper-Promoted C-Se Bond Formation: Synthesis of 2-Alkyl-1,2-benzisoselenazol-3(2H)-ones and Evaluation against Mycobacterium tuberculosis. J Org Chem. 2017 Apr 7;82(7):3844-3854.
Barreto JG, Frade MAC, Bernardes Filho F, da Silva MB, Spencer JS, Salgado CG. Leprosy in Children. Curr Infect Dis Rep. 2017 Jun;19(6):23. doi: 10.1007/s11908-017-0577-6.
Lee JS, Mackie RS, Harrison T, Shariat B, Kind T, Kehl T, Löchelt M, Boucher C, VandeWoude S. Targeted enrichment for pathogen detection and characterization in three felid species. J Clin Microbiol. 2017 Mar 22. pii: JCM.01463-16. doi: 10.1128/JCM.01463-16.
Bon Voyage to Ian and Jenny
Friday May 12th marked the end of an era in MIP. University Distinguished Professor Ian Orme began his transitional retirement and his long-time trusted Research Coordinator Jenny Harding left the Department to take a similar position in the College of Engineering.
After receiving his BS and PhD degrees (accompanied, we believe by a few pints along the way) from the University of London, Ian took a postdoctoral position at the Trudeau Institute in New York. In 1986 - the year that saw the Chernobyl meltdown, the Challenger Space Shuttle accident, Diego Maradona’s infamous Hand of God goal, and Bill Buckner’s error that helped the Mets beat the Red Sox in the World Series – Ian started his career at CSU as an Assistant Professor. He was promoted to Full Professor in 1995 and named a University Distinguished Professor in 2009. Ian has published over 300 papers in his career to date and is an elected Fellow of the ASM and the AAAS. He will be back this summer and for the next several years on a part time basis as he transitions to full retirement.
Jenny has worked almost 23 years with Ian, helping him crank out hundreds of papers and many, many millions of dollars in grant proposals. Jenny received her BA in Sociology from CSU and an invaluable extensive education in British rugby, football and other antics from Ian. She has been a key contributor to the MRL and MIP and will be sorely missed.
Congratulations and Best of Luck!
Congratulations Amy Scharf!
Congratulations to Amy Scharf on graduating with a bachelor’s degree in Biomedical Sciences! Amy has been a student coordinator in the Microbiology Front Office since January 2015. She has served as a departmental courier, assisted with travel, PCard reallocation, purchasing, provided facilities assistance, maintained the external website, been an instrumental part in developing the intranet, and has provided superior customer service to our staff, faculty, and students for the last two and a half years. We thank Amy for her service to MIP and congratulate her on her degree (even if it wasn’t in a subject as cool as Microbiology….)!
MIP Grad Students Help High Schoolers Qualify for the National Science Olympiad Finals
The Fossil Ridge HS teams coached by four MIP grad students (Reyes Murrieta, Shaun Cross, Megan Miller and Bridget Eklund) came in 1st and 5th for Disease Detectives and 3rd and 4th for Microbe Mission in the State Science Olympiad Tournament on April 15. There were a total of 27 teams competing. The FRHS team came in first overall and qualified to go to Ohio for the National Tournament on May 20th.
MIP150 Research Symposium Gives Underclassman Experience in Poster Presentations
On May 1st students from our Intro to Research Methods course presented their first scientific posters ever in Microbiology A201.
Congrats to all!!
TB Doesn’t Have To Be – World TB Day 2017
On March 24, 1882, Robert Koch discovered Mycobacterium tuberculosis, the causative agent of tuberculosis (TB). Today, TB is one of the top ten causes of death worldwide. Every day, over 450 children alone are diagnosed with TB. To bring awareness to this infectious disease and rally support to once and for all contain the TB epidemic, every March 24th has been designated ‘World TB Day’. The theme for this year’s event was ‘Unite to End TB’. Our Mycobacterial Research Laboratories once again held a major rally and educational campaign on campus. It was a huge success! Check out all the photos here.
Microbiology Student Association Happenings
New MSA Board
MSA Board Adds Some Culture to the Town
MSA Brewery Tour
MSA got a great ‘behind the scenes’ tour of Odell's Brewery to see some microbes in action.
MIP Goes the Extra Mile in the March for Science
Cilck here for more images.
CURC Practice Night
MIP held its first ever Celebrate Undergraduate Research and Creativity (CURC) symposium practice night hosted by the MIP Undergraduate Research Fellows. Twenty students got an opportunity to practice their presentations and have their posters edited by many viewers. Given the success of MIP posters at the Symposium, the practice clearly paid off!!
3rd Annual Front Range Computational & Systems Biology Symposium: Microbiome
The symposium will take place June 12-13th. Click here for more information, to register, submit a poster presentation and request a lunch.
Click here if you like unpronounceable 7 letter acronyms.
2nd International Symposium on Infectious Diseases of Bats
The 2nd International Bat Infectious Disease meeting hosted by our own Tony Schountz will take place on June 29-July 1, 2017 at the University Center for the Arts here at CSU.
Clicke here for more information.
72nd Annual International Conference on Diseases in Nature Communicable to Man
The 72nd Annual International Conference on Diseases in Nature Communicable to Man
Click here for more information.
ComSciCon is a two-day event focused on science communication and policy that is free and open to all graduate students and post-doctoral researchers in STEM departments.
Click here for the application.
More Universal Visions from Our own ‘Guardian of the Galaxy’ Mike Scherman
Check out these intimate views of Jupiter, the Orion and Running Man Nebulae and some sun spots taken by Mike.
Horsetooth Half Marathon
Ben Curtis, Kendra Andrie, Gary Mason, Jenn Malmberg, Lauren Harris, and Allison Vilander are all smiles after completing the Horsetooth Half Marathon in April.
Professor Emeritus/Photographer Ralph Smith On Display
Ralph Smith will have photographs on display during the month of May at the di Cincotta coffee shop located at Sprouts Market (Drake and Lemay). Stop by to see more photos!
Adriano de Matos e Silva (from John Belisle’s lab) and Zitely Tzompa Sosa (Ph.D. candidate Atmospheric Sciences) were married in Mexico on April 9th. We wish the couple all the happiness in the world!
"Ears really are an underrated organ."
-Tony Frank, CSU President
Choose your Caption
Mark Zabel Marches for Science
1.Check out the dude wearing his undergrad GPA on his sleeve…
2.Hmmmm. I can think of a few hypothesis of mine that could be put into the Mother of All Bombs category…
3. For God Particle’s sake, fund this guy!
In Case You Missed It….
Check out what it says on the upper right corner of the blackboard in the photo used by CSU-Source in the article on the Rovnak Team’s Zika advance
Ever wonder if you are eligible for continuous NIH grant submission?
New NIH policy allows basically nothing in the appendix section of a grant application unless its asked for by the FOA
NIGMS announces an upcoming new T32 training grant program designed to modernize graduate education.
New Grant Awards
Randall Basaraba: "Disrupting Biofilm Formation to Improve TB Drug Treatment," HHS-NIH-NIAID-Allergy and Infect Diseases. "Core D: Metabolic Factors that Control the Spectrum of Human Tuberculosis," Brigham and Womens Hospital. "Project 3: Metabolic Factors that Control the Spectrum of Human Tuberculosis," Brigham and Womens Hospital."Project 2: Metabolic Factors that Control the Spectrum of Human Tuberculosis," Brigham and Womens Hospital. "Project 1: Metabolic Factors that Control the Spectrum of Human Tuberculosis," Brigham and Womens Hospital.
John Belisle: "BMAC CSU Core Facility Mass Spectrometry Services," HHS-CDC-Centers for Disease Control.
Delphi Chatterjee: "Role of Gamma/Delta T Cells in Vaccine Induced Immunity," Saint Louis University.
Dean Crick: "Menaquinone Biosynthesis: A Drug Target in Gram-Positive Bacteria," HHS-NIH-NIAID-Allergy and Infect Diseases.
Gregg Dean, Kelly Santangelo: "Proteomic Analysis of Plasma to Identify Prognostic and/or Diagnostic Biomarkers of Feline Infectious Peritonitis," Winn Feline Foundation.
Karen Dobos, Martha Mehaffy: "Mycobacterium spp. Research Reagent Replenishment," American Type Culture Collection.
Greg Ebel: "Predicting Genetic Determinants of Zika Virus Emergence," HHS-NIH-NIAID-Allergy and Infect Diseases.
Mercedes Gonzalez: "Treatment of NTM Respiratory Infections with Inhaled Drug Delivery," Aerophase, Inc. "Aerosol Spectinamide-1599 Therapy Against Tuberculosis," HHS-NIH-NIAID-Allergy and Infect Diseases.
Anne Lenaerts, Greg Robertson: "TB Drug Accelerator: TB Mouse in vivo Models," Bill and Melinda Gates Foundation .
Ken Olson: "Next-Generation, Semi-Orthogonal eTIPs for Active Suppression of Alpha Virus Replications," University of Washington. "Nanowire-Based Assay for Detection of Dengue and Chikungunya Virus," InnoSense, LLC.
Christine Olver: "Mitigation of snake envenomation using an alternative therapy on canine whole blood exposed to hemotoxic venom," Morris Animal Foundation. "The reduction of Plasmodium falciparum using the Mirasol PRT System for Whole Blood as measured by an in vitro cell culture assay," Terumo BCT Biotechnologies, LLC.
Daniel Regan, Daniel Gustafson, Steven Dow: "Elucidating the Role and Regulation of Periostin in Therapy Induced Chemoresistance in Metastatic Breast Cancer," HHS-NIH-NCI-National Cancer Institute.
William Schountz: "2nd International Infectious Diseases of Bats Symposium," HHS-NIH-National Institutes of Health.
Glenn Telling: "Fyn Activation in Prion Disease," HHS-NIH-NIAID-Allergy and Infect Diseases.
Sue VandeWoude, William Funk: "Impacts of Landscape Structure, Host Demography, and Management Interventions on Disease Dynamics," NSF-National Science Foundation.
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