July 2014
Vol. 11 | No. 3

National Academy of Sciences
Ed Hoover NSA award

Congratulations to Dr. Ed Hoover for being elected to the prestigious National Academy of Sciences. The Academy was established under a congressional charter signed by President Abraham Lincoln in 1863 and is a private, nonprofit institution which recognizes distinguished and continuing achievements in original research. Dr. Hoover is one of 84 newly elected members and one of 9 CSU faculty members elected to the Academy. Read the full story at Today @ Colorado State. Nicely done Ed!

Promotion and tenure actions

Congratulations to the following faculty on their upcoming promotion and/or tenure actions which became effective July 1, 2014:

Mary JacksonMary Jackson
EJ Ehrhart EJ Ehrhart
Anne LenaertsAnne Lenaerts
Diane OrdwayDiane Ordway
Associate Professor (special appointment)

Associate Director of RICRO

Karen Dobos lab picture MIP Associate Professor, Dr. Karen Dobos, has accepted a position as the Associate Director in the Research Integrity & Compliance Review Office (RICRO). Don't worry though, she is not giving up her research. She will continue with her tuberculosis vaccine research, while providing leadership to the RICRO staff. Congratulations Karen!

Zoetis Distinguished
Veterinary Teacher Award

Gary Mason headshot

Dr. Gary Mason was recently awarded the Zoetis Distinguished Veterinary Teacher Award. This award is given to educators in recognition of their character and leadership qualities as well as their outstanding teaching abilities. Congratulations Gary!

Journal of Immunology Logo

Alan Schenkel headshot

Dr. Alan Schenkel has been promoted to Associate Editor at the Journal of Immunology. Way to go Alan!

Big Love for Becky
Becky Trentlage

On April 22, Becky Trentlage, MIPs Business Officer, was recognized and presented with the first overall CVMBS Living our Values award, which focuses on Transparency, Accountability, Collaboration, and Team. Becky's nominator had this to say: Becky is actively pursuing the implementation of the College’s shared values. She looks at the impacts of decisions on all members of the departmental team, research teams, and academic teams. She has the ability to see the short-term impacts and long-term visions. She strives daily to bring her team together to maximize efficiencies and effectiveness. She recognizes that strong teams are built on individual efforts and recognition of effort, no matter how small.Well done Becky!

Congrats Residents

Congratulations to the following students who will be completing their Veterinary Residency Program this year:

Ryan Curtis

Ryan Curtis
Comparative Medicine

Elijah Edmondson

Elijah Edmondson
Anatomic Pathology

Ashley Malmlov

Ashley Malmlov

Dan Regan

Dan Regan
Anatomic Pathology

April White

April White
Clinidcal Pathology

Seung Yoo

Seung Yoo
Clinical Pathology

A reception was held on June 10th to congratulate each resident on their service committment to CSU Pathology. View the photo gallery from the reception.

Micro Undergrads
In the News...

Sarah Nelson, an MIP student was recently featured in her hometown paper, Hot Springs Star. Sarah has been selected to participate in the "Doctor Interns" program, a non-profit organization that takes students on a two week internship to Shree Prannath Hospital in Surat, India. While there, Sarah will have the opportunity to rotate among a team of five doctors with different specialites. Congratulations Sarah!

Phi Kappa Phi

Phi Kappa Phi Outstanding Junior Award

Taylor Farley Congratulations to Taylor Farley, an undergrad in MIP for receiving the Phi Kappa Phi Outstanding Junior award. Phi Kappa Phi was established to recognize juniors in college who have demonstrated outstanding achievement and who show excellent promise to distinguish themselves as they finish programs of study and move beyond Colorado State University. To qualify for this award students must rank in the top 7.5% of their class and be nominated by a faculty member in their area of study. Way to go Taylor!

Dr. Mark Stenglein signs on the
Bottom Line

We are pleased to announce that Dr. Mark Stenglein will be joining MIP September 1 as an Assistant Professor in the new Computational Biologist position that was recently created. Welcome aboard Mark!

Outstanding Specialty Resident Award

Krafsur Outstanding Resident awardGreta Krafsur

Greta Krafsur, a resident in the Anatomic Pathology Program, recently received the Outstanding Specialty Resident award. This award is given for invaluable teaching and clinical support of CSU Veterinary students. Way to go Greta!

2014 CURC Symposium

The annual Celebrate Undergraduate Research and Creativity (CURC) Symposium was held on April 15th in Johnson Hall. Several MIP students participated and won awards in this years event.

MIP Winners of the 2014 CURC Symposium:

Amber Mayfield
Highest Honors
mentor: Candace Mathiason

Wojtek Kuklinski
High Honors
mentor: Brian Foy

Monica Brandhuber
High Honors
mentor: Candace Mathiason

Guy Stewart
College Honors
mentor: Lucas Argueso

Miya Holley
College Honors
mentor: RoxAnn Karkhoff-Schweizer

Kristina Glapa
College Honors
mentor: EJ Ehrhart

Amber Mayfield and Monica Brandhuber 2014 CURC winners

Amber Mayfield and Monica Brandhuber with their 2014 CURC winner certificates.

Check out the CURC Photo Gallery.

Congratulations Graduates

Congratulations Micro BS Program Graduates

Jacob Ashby
Cory Berens
Cheryl Bergman
Sara Bikab
Sarah Born
Amanda Brown
Kaylee Brown
Victor Cheng
Tyler Dalby
Aaron Deden (Sm14)
Lora Dreesen
Edward Ettema (Sm14)
Victoria Frank
Yanwen Gong
Reese Hitchings
Miya Holley
Taryn Holliday
Kevne Hull
Mark Jeon
Melissa Karres
Nicole Kelly
Ryan Kennon
Sarah Kirchner
Christina Kruk
Tyler Maxwell
Angela Niebergall
Tara Pianko
Hannah Pirner
Joel Rivera (Sm14)
Kayla Sible
Hannah Skaggs
Kerry Spring
Alexander Starekow
Ashley Wengrove
Jessica Wright
Benjamin Zeter

Lora Dressen graduation Spring 2014

Joel Rivera, Angela Niebergall, mark Jeon, Aaron Deden, Lora Dressen and Ted Ettema celebrate their graduation.

Congratulations to the first class of Micro MS-B Program Graduates

Al Aradi
Kristine Breivik
Josh Brown
Carl Dernell
Vickie Ektnitphong
Jordan Flatt
Lauren Foster
Emma Gardner
Andy Gonzalez
Joe Guy
Christie Hannum
Shay Harre
Samara Licholat
Katie Menning
Kristin Neal
Sean Pellow
Mariah Pippins
Paul Simmons
Lauren Tauer
Tanvi Tijoriwala
Alex Walser
Shannon Wittstock
Steven Woods
Cassie Zych

First class of MS-B Program Graduates Spring 2014

Dean's List Spring 2014

Kaylee Brown
Roslyn Dermody
Tess Ehrhart Taylor Farley
Gladys Hill
Miya Holley
Caitlin Lozano
John Rogers
Sarah Sullivan
Victoria Taillac
Lily Won

pub highlight

A hearty congratulations to MIP labs for producing a whopping 73 manuscripts that appeared on Pub Med between February and June of this year! With so many great papers out there in the literature, how can we highlight just one in this belated MIPnews issue? Thus here’s a brief description of four noteworthy papers that appeared this Spring. Frankly we wish there was space to highlight at least half a dozen more……

Development of an in vitro model of acquired resistance to toceranib phosphate (Palladia®) in canine mast cell tumor.

Halsey CH, Gustafson DL, Rose BJ, Wolf-Ringwall A, Burnett RC, Duval DL, Avery AC, Thamm DH.

BMC Vet Res. doi: 10.1186/1746-6148-10-105.

When you are trying to understand and eventually cure dog mast cell tumors – the most common neoplasms in the canine planet – your toolbox can never be big enough. Drugs that target the Kit tyrosine kinase have been approved to treat these tumors, but the development of resistance in the tumor cells being treated is a common problem that ultimately leads to adverse clinical outcomes. In this collaborative study, MIP and CS researchers have developed a mast cell tumor line that mimics a drug-resistant mast cell tumor. This novel reagent should help researchers not only understand drug resistance mechanisms, but also may serve as a tool in the investigation of ways to treat KIT inhibitor-resistant tumors in our furry friends.

A trisubstituted benzimidazole cell division inhibitor with efficacy against Mycobacterium tuberculosis.

Knudson SE, Awasthi D, Kumar K, Carreau A, Goullieux L, Lagrange S, Vermet H, Ojima I, Slayden RA.

PLoS One, 9(4):e93953

This paper provides on the latest update on the use of trisubstituted benzimidazoles as a platform to develop a novel class of drugs to combat M. tuberculosis infections. By exploiting structure-activity relationships, Ric and the gang have come up with a new lead compound that can reduce mycobacterial replication in the lungs. In other words, the Mtb infected mice in the study breathed a ‘sigh of relief’ – and hopefully this and/or related compounds will continue to perform well along the many steps in the drug development pipeline.

Quinacrine promotes replication and conformational mutation of chronic wasting disease prions.

Bian J, Kang HE, Telling GC.

Proc Natl Acad Sci U S A., 111:6028-33

Developing drugs to treat prion diseases has been a frustrating experience to say the least. The drug quinacrine was in clinical development for the better part of a decade before it was determined that it simply didn’t work as advertised. In this study, Glenn and the lab closely examined the effect of this drug on prions and found that quinacrine appears to force the prion protein into a specific conformation that can actually increase the multiplication rate for certain prion agents/strains rather than reduce it. This observation provides extremely valuable information to instruct how anti-prion drugs should be screened. Since prion drug discovery, like much of laboratory science, is only as good/successful as the quality of the assay used in the experiment, the results in this study may be a very powerful wake up call.

The structural basis of pathogenic subgenomic flavivirus RNA (sfRNA) production.

Chapman EG, Costantino DA, Rabe JL, Moon SL, Wilusz J, Nix JC, Kieft JS.

Science, 344:307-10.

In this collaborative study that grew out of interactions at a meeting of the Rocky Mountain Virology Club in Pingree Park, the Kieft lab has solved a very unique structure in the flavivirus genomic RNA that blocks and ultimately represses a major exonuclease involved in cellular mRNA decay. The repression of this enzyme by the RNA structure stabilizes viral RNA and significantly dysregulates cellular gene expression which may contribute to viral-induced cytopathology. This fascinating knot-like structure (which made the cover of the 2014 RNA Society Meeting abstract book) is a very attractive drug target and opens the door for a detailed molecular understanding of an understudied area of virus-host interaction.

MIP Publications May 2014

Soffler C, Bosco-Lauth AM, Aboellail TA, Marolf AJ, Bowen RA. Pathogenesis of percutaneous infection of goats with Burkholderia pseudomallei: clinical, pathologic, and immunological responses in chronic melioidosis. Int J Exp Pathol. 2014 Apr;95(2):101-19. doi: 10.1111/iep.12068.

Engel S, Randall EK, Cuddon PA, Webb BT, Aboellail TA. Imaging diagnosis: Multiple cartilaginous exostoses and calcinosis circumscripta occurring simultaneously in the cervical spine of a dog. Vet Radiol Ultrasound. 2014 May-Jun;55(3):305-9. doi: 10.1111/vru.12066. Epub 2013 Jun 14.

Halsey CH, Gustafson DL, Rose BJ, Wolf-Ringwall A, Burnett RC, Duval DL, Avery AC, Thamm DH. Development of an in vitro model of acquired resistance to toceranib phosphate (Palladia®) in canine mast cell tumor. BMC Vet Res. 2014 May 6;10(1):105. doi: 10.1186/1746-6148-10-105.

Seelig DM, Avery P, Webb T, Yoshimoto J, Bromberek J, Ehrhart EJ, Avery AC. Canine T-zone lymphoma: unique immunophenotypic features, outcome, and population characteristics. J Vet Intern Med. 2014 May;28(3):878-86.

Ballweber LR, Beugnet F, Marchiondo AA, Payne PA. American Association of Veterinary Parasitologists' review of veterinary fecal flotation methods and factors influencing their accuracy and use-Is there really one best technique? Vet Parasitol. 2014 May 17. pii: S0304-4017(14)00288-X. doi: 10.1016/j.vetpar.2014.05.009.

Lishnevsky M, Young LC, Woods SJ, Groshong SD, Basaraba RJ, Gilchrist JM, Higgins DM, Gonzalez-Juarrero M, Bass TA, Muller WA, Schenkel AR. Microhemorrhage is an early event in the pulmonary fibrotic disease of PECAM-1 deficient FVB/n mice. Exp Mol Pathol. 2014 Jun 24. pii: S0014-4800(14)00092-6. doi: 10.1016/j.yexmp.2014.06.008.

Bartek IL, Woolhiser LK, Baughn AD, Basaraba RJ, Jacobs WR Jr, Lenaerts AJ, Voskuil MI. Mycobacterium tuberculosis Lsr2 is a global transcriptional regulator required for adaptation to changing oxygen levels and virulence. MBio. 2014 Jun 3;5(3):e01106-14. doi: 10.1128/mBio.01106-14.

Shikani AH, Sidhaye VK, Basaraba RJ, Shikani HJ, Alqudah MA, Kirk N, Cope E, Leid JG. Mucosal expression of aquaporin 5 and epithelial barrier proteins in chronic rhinosinusitis with and without nasal polyps. Am J Otolaryngol. 2014 May-Jun;35(3):377-83. doi: 10.1016/j.amjoto.2013.11.011.

Ackart DF, Hascall-Dove L, Caceres SM, Kirk NM, Podell BK, Melander C, Orme IM, Leid JG, Nick JA, Basaraba RJ. Expression of antimicrobial drug tolerance by attached communities of Mycobacterium tuberculosis. Pathog Dis. 2014 Apr;70(3):359-69. doi: 10.1111/2049-632X.12144.

Baak-Baak CM, Arana-Guardia R, Cigarroa-Toledo N, Loroño-Pino MA, Reyes-Solis G, Machain-Williams C, Beaty BJ, Eisen L, García-Rejón JE. Vacant lots: productive sites for Aedes (Stegomyia) aegypti (Diptera: Culicidae) in Mérida City, México. J Med Entomol. 2014 Mar;51(2):475-83.

Arana-Guardia R, Baak-Baak CM, Loroño-Pino MA, Machain-Williams C, Beaty BJ, Eisen L, García-Rejón JE. Stormwater drains and catch basins as sources for production of Aedes aegypti and Culex quinquefasciatus. Acta Trop. 2014 Jun;134:33-42.

Porta J, Jose J, Roehrig JT, Blair CD, Kuhn RJ, Rossmann MG. Locking and Blocking the Viral Landscape of an Alphavirus with Neutralizing Antibodies. J Virol. 2014 Jun 11. pii: JVI.01286-14.

Chotiwan N, Roehrig JT, Schlesinger JJ, Blair CD, Huang CY. Molecular determinants of dengue virus 2 envelope protein important for virus entry in FcγRIIA-mediated antibody-dependent enhancement of infection. Virology. 2014 May;456-457:238-46. doi: 10.1016/j.virol.2014.03.031.

Skalsky RL, Olson KE, Blair CD, Garcia-Blanco MA, Cullen BR. A "microRNA-like" small RNA expressed by Dengue virus? Proc Natl Acad Sci U S A. 2014 Jun 10;111(23):E2359. doi: 10.1073/pnas.1406854111.

Shearin H, Bessen RA. Axonal and transynaptic spread of prions. J Virol. 2014 May 21. pii: JVI.00378-14.

Lopez B, Ponce G, Gonzalez JA, Gutierrez SM, Villanueva OK, Gonzalez G, Bobadilla C, Rodriguez IP, Black WC 4th, Flores AE. Susceptibility to chlorpyrifos in pyrethroid-resistant populations of Aedes aegypti (Diptera: Culicidae) from Mexico. J Med Entomol. 2014 May;51(3):644-9.

Schountz T, Quackenbush S, Rovnak J, Haddock E, Black WC 4th, Feldmann H, Prescott J. Differential Lymphocyte and Antibody Responses in Deer Mice Infected with Sin Nombre or Andes Hantaviruses. J Virol. 2014 May 14. pii: JVI.00004-14.

Franz AW, Sanchez-Vargas I, Raban RR, Black WC 4th, James AA, Olson KE. Fitness impact and stability of a transgene conferring resistance to dengue-2 virus following introgression into a genetically diverse Aedes aegypti strain. PLoS Negl Trop Dis. 2014 May 8;8(5):e2833. doi: 10.1371/journal.pntd.0002833.

Brandt LE, Ehrhart EJ, Scherman H, Olver CS, Bohn AA, Prenni JE. Characterization of the canine urinary proteome. Vet Clin Pathol. 2014 Apr 28. doi: 10.1111/vcp.12147. [Epub ahead of print]

Emanuelli MP, Bohn AA. What is your diagnosis? Dermal mass in a dog. Vet Clin Pathol. 2014 Mar 3. doi: 10.1111/vcp.12130. [Epub ahead of print]

Rivoire BL, Groathouse NA, TerLouw S, Neupane KD, Ranjit C, Sapkota BR, Khadge S, Kunwar CB, Macdonald M, Hawksworth R, Thapa MB, Hagge DA, Tibbals M, Smith C, Dube T, She D, Wolff M, Zhou E, Makhene M, Mason R, Sizemore C, Brennan PJ. Safety and efficacy assessment of two new leprosy skin test antigens: randomized double blind clinical study. PLoS Negl Trop Dis. 2014 May 29;8(5):e2811. doi: 10.1371/journal.pntd.0002811

Rivoire BL, TerLouw S, Groathouse NA, Brennan PJ. The Challenge of Producing Skin Test Antigens with Minimal Resources Suitable for Human Application against a Neglected Tropical Disease; Leprosy. PLoS Negl Trop Dis. 2014 May 29;8(5):e2791. doi: 10.1371/journal.pntd.0002791.

Li K, Schurig-Briccio LA, Feng X, Upadhyay A, Pujari V, Lechartier B, Fontes FL, Yang H, Rao G, Zhu W, Gulati A, No JH, Cintra G, Bogue S, Liu YL, Molohon K, Orlean P, Mitchell DA, Freitas-Junior L, Ren F, Sun H, Jiang T, Li Y, Guo RT, Cole ST, Gennis RB, Crick DC, Oldfield E. Multitarget Drug Discovery for Tuberculosis and Other Infectious Diseases. J Med Chem. 2014 Apr 1. [Epub ahead of print]

Perley CC, Frahm M, Click EM, Dobos KM, Ferrari G, Stout JE, Frothingham R. The Human Antibody Response to the Surface of Mycobacterium tuberculosis. PLoS One. 2014 Jun 11;9(2):e98938. doi: 10.1371/journal.pone.0098938.

Duncan CG, Tiller R, Mathis D, Stoddard R, Kersh GJ, Dickerson B, Gelatt T. Brucella placentitis and seroprevalence in northern fur seals (Callorhinus ursinus) of the Pribilof Islands, Alaska. J Vet Diagn Invest. 2014 May 6.

McLeland SM, Lunn KF, Duncan CG, Refsal KR, Quimby JM. Relationship among Serum Creatinine, Serum Gastrin, Calcium-phosphorus Product, and Uremic Gastropathy in Cats with Chronic Kidney Disease. J Vet Intern Med. 2014 May;28(3):827-37.

Flynn P, Duncan CG, Palmer RH, Duerr FM. In Vitro Incidence of Fibular Penetration With and Without the Use of a Jig During Tibial Plateau Leveling Osteotomy. Vet Surg. 2014 Mar 11. doi: 10.1111/j.1532-950X.2014.12152.x. [Epub ahead of print]

Wieczorek AE, Troudt JL, Knabenbauer P, Taylor J, Pavlicek RL, Karls R, Hess A, Davidson RM, Strong M, Bielefeldt-Ohmann H, Izzo AA, Dobos KM. HspX vaccination and role in virulence in the guinea pig model of tuberculosis. Pathog Dis. 2014 Feb 24. doi: 10.1111/2049-632X.12147. [Epub ahead of print]

Ebel GD. Toward an activist agenda for monitoring virus emergence. Cell Host Microbe. 2014 Jun 11;15(6):655-6. doi: 10.1016/j.chom.2014.05.014.

Eisen L, Monaghan AJ, Lozano-Fuentes S, Steinhoff DF, Hayden MH, Bieringer PE. The impact of temperature on the bionomics of Aedes (Stegomyia) aegypti, with special reference to the cool geographic range margins. J Med Entomol. 2014 May;51(3):496-516.

Shoeneman JK, Ehrhart EJ 3rd, Charles JB, Thamm DH. Survivin inhibition via EZN-3042 in canine lymphoma and osteosarcoma. Vet Comp Oncol. 2014 Jun 13. doi: 10.1111/vco.12104.

Edmondson EF, Bright JM, Halsey CH, Ehrhart EJ. Pathologic and Cardiovascular Characterization of Pheochromocytoma-Associated Cardiomyopathy in Dogs. Vet Pathol. 2014 May 8.

Paoloni M, Webb C, Mazcko C, Cherba D, Hendricks W, Lana S, Ehrhart EJ, Charles B, Fehling H, Kumar L, Vail D, Henson M, Childress M, Kitchell B, Kingsley C, Kim S, Neff M, Davis B, Khanna C, Trent J. Prospective molecular profiling of canine cancers provides a clinically relevant comparative model for evaluating personalized medicine (PMed) trials. PLoS One. 2014 Mar 17;9(3):e90028. doi: 10.1371/journal.pone.0090028.

Halsey CH, Worley DR, Curran K, Charles JB, Ehrhart EJ. The use of novel lymphatic endothelial cell-specific immunohistochemical markers to differentiate cutaneous angiosarcomas in dogs. Vet Comp Oncol. 2014 Mar 5. doi: 10.1111/vco.12088. [Epub ahead of print]

Gadomski BC, McGilvray KC, Easley JT, Palmer RH, Ehrhart EJ, Haussler KK, Browning RC, Santoni BG, Puttlitz CM. An in vivo ovine model of bone tissue alterations in simulated microgravity conditions. J Biomech Eng. 2014 Feb;136(2):021020. doi: 10.1115/1.4025854.

Haley NJ, Carver S, Hoon-Hanks L, Henderson DM, Davenport KA, Bunting E, Gray S, Trindle B, Galeota J, LeVan I, Dubovos T, Shelton P, Hoover EA. Chronic wasting disease detection in the lymph nodes of free-ranging cervids by real time quaking-induced conversion. J Clin Microbiol. 2014 Jun 23. pii: JCM.01258-14.

Schurich JA, Kumar S, Eisen L, Moore CG. Modeling Culex tarsalis abundance on the northern Colorado front range using a landscape-level approach. J Am Mosq Control Assoc. 2014 Mar;30(1):7-20.

Springer YP, Eisen L, Beati L, James AM, Eisen RJ. Spatial distribution of counties in the continental United States with records of occurrence of Amblyomma americanum (Ixodida: Ixodidae). J Med Entomol. 2014 Mar;51(2):342-51.

Slade JR, Mulligan RT, Labrecque B, Kobylinski KC, Gray M, Kuklinski WS, Burton TA, Seaman JA, Sylla M, Foy BD. Design and testing of a novel, protective human-baited tent trap for the collection of anthropophilic disease vectors. J Med Entomol. 2014 Jan;51(1):253-63.

Seelig DM, Nalls AV, Flasik M, Frank V, Eaton S, Mathiason CK, Hoover EA. Lesion Profiling and Subcellular Prion Localization of Cervid Chronic Wasting Disease in Domestic Cats. Vet Pathol. 2014 Feb 27. [Epub ahead of print]

Torres-Henderson C, Hesser J, Hyatt DR, Hawley J, Brewer M, Lappin MR. Pilot study to evaluate the role of Mycoplasma species in cat bite abscesses. J Feline Med Surg. 2014 Mar 18. [Epub ahead of print]

Yang L, Lu S, Belardinelli J, Huc-Claustre E, Jones V, Jackson M, Zgurskaya HI. RND transporters protect Corynebacterium glutamicum from antibiotics by assembling the outer membrane. Microbiologyopen. 2014 Jun 18. doi: 10.1002/mbo3.182.

Angala SK, Belardinelli JM, Huc-Claustre E, Wheat WH, Jackson M. The cell envelope glycoconjugates of Mycobacterium tuberculosis. Crit Rev Biochem Mol Biol. 2014 Jun 10:1-39.

Puckett S, Trujillo C, Eoh H, Marrero J, Spencer J, Jackson M, Schnappinger D, Rhee K, Ehrt S. Inactivation of fructose-1,6-bisphosphate aldolase prevents optimal co-catabolism of glycolytic and gluconeogenic carbon substrates in Mycobacterium tuberculosis. PLoS Pathog. 2014 May 22;10(5):e1004144. doi: 10.1371/journal.ppat.1004144.

Svetlíková Z, Baráth P, Jackson M, Korduláková J, Mikušová K. Purification and characterization of the acyltransferase involved in biosynthesis of the major mycobacterial cell envelope glycolipid - Monoacylated phosphatidylinositol dimannoside. Protein Expr Purif. 2014 Aug;100:33-9. doi: 10.1016/j.pep.2014.04.014.

Burgess BA, Noyes NR, Bolte DS, Hyatt DR, van Metre DC, Morley PS. Rapid Salmonella detection in experimentally inoculated equine faecal and veterinary hospital environmental samples using commercially available lateral flow immunoassays. Equine Vet J. 2014 Feb 9. doi: 10.1111/evj.12234. [Epub ahead of print]

Calado Nogueira de Moura V, Gibbs S, Jackson M. Gene Replacement in Mycobacterium chelonae: Application to the Construction of Porin Knock-Out Mutants. PLoS One. 2014 Apr 16;9(4):e94951. doi: 10.1371/journal.pone.0094951.

Tettelin H, Davidson RM, Agrawal S, Aitken ML, Shallom S, Hasan NA, Strong M, de Moura VC, De Groote MA, Duarte RS, Hine E, Parankush S, Su Q, Daugherty SC, Fraser CM, Brown-Elliott BA, Wallace RJ Jr, Holland SM, Sampaio EP, Olivier KN, Jackson M, Zelazny AM. High-level relatedness among Mycobacterium abscessus subsp. massiliense strains from widely separated outbreaks. Emerg Infect Dis. 2014 Mar;20(3):364-71.

Irwin SM, Gruppo V, Brooks E, Gilliland J, Scherman M, Reichlen MJ, Leistikow R, Kramnik I, Nuermberger EL, Voskuil MI, Lenaerts AJ. Limited Activity of Clofazimine as a Single Drug in a Mouse Model of Tuberculosis Exhibiting Caseous Necrotic Granulomas. Antimicrob Agents Chemother. 2014 Jul;58(7):4026-4034.

Rice AD, Adams MM, Lindsey SF, Swetnam DM, Manning BR, Smith AJ, Burrage AM, Wallace G, MacNeill AL, Moyer RW. Protective Properties of Vaccinia Virus-Based Vaccines: Skin Scarification Promotes a Nonspecific Immune Response That Protects against Orthopoxvirus Disease. J Virol. 2014 Jul 15;88(14):7753-63. doi: 10.1128/JVI.00185-14.

Nathaniel R, MacNeill AL, Wang YX, Turner PC, Moyer RW. Cowpox virus CrmA, Myxoma virus SERP2 and baculovirus P35 are not functionally interchangeable caspase inhibitors in poxvirus infections. J Gen Virol. 2004 May;85(Pt 5):1267-78.

Shinoda H, Legare ME, Mason GL, Berkbigler JL, Afzali MF, Flint AF, Hanneman WH. Significance of ERα, HER2, and CAV1 expression and molecular subtype classification to canine mammary gland tumor. J Vet Diagn Invest. 2014 Apr 23. [Epub ahead of print]

Rout ED, Webb TL, Laurence HM, Long L, Olver CS. Transferrin receptor expression in serum exosomes as a marker of regenerative anaemia in the horse. Equine Vet J. 2014 Feb 12. doi: 10.1111/evj.12235. [Epub ahead of print]

Orme IM, Ordway DJ. The host response to non-tuberculous mycobacterial infections of current clinical importance. Infect Immun. 2014 Jun 9. pii: IAI.01606-13.

Scotch M, Lam TT, Pabilonia KL, Anderson T, Baroch J, Kohler D, DeLiberto TJ. Diffusion of influenza viruses among migratory birds with a focus on the Southwest United States. Infect Genet Evol. 2014 Jun 6;26C:185-193. doi: 10.1016/j.meegid.2014.05.029.

Edmondson EF, Hess AM, Powers BE. Prognostic Significance of Histologic Features in Canine Renal Cell Carcinomas: 70 Nephrectomies. Vet Pathol. 2014 May 14. pii: 0300985814533803.

Santangelo KS, Kaeding AC, Baker SA, Bertone AL. Quantitative Gait Analysis Detects Significant Differences in Movement between Osteoarthritic and Nonosteoarthritic Guinea Pig Strains before and after Treatment with Flunixin Meglumine. Arthritis. 2014;2014:503519. doi: 10.1155/2014/503519.

Knudson SE, Kumar K, Awasthi D, Ojima I, Slayden RA. In vitro-in vivo activity relationship of substituted benzimidazole cell division inhibitors with activity against Mycobacterium tuberculosis. Tuberculosis (Edinb). 2014 Apr 1. pii: S1472-9792(14)20389-1. doi: 10.1016/j.tube.2014.03.007. [Epub ahead of print]

Schiebel J, Chang A, Shah S, Lu Y, Liu L, Pan P, Hirschbeck MW, Tareilus M, Eltschkner S, Yu W, Cummings JE, Knudson SE, Bommineni GR, Walker SG, Slayden RA, Sotriffer CA, Tonge PJ, Kisker C. Rational Design of Broad-Spectrum Antibacterial Activity based on a Clinically Relevant Enoyl-ACP Reductase Inhibitor. J Biol Chem. 2014 Apr 16. [Epub ahead of print]

Knudson SE, Awasthi D, Kumar K, Carreau A, Goullieux L, Lagrange S, Vermet H, Ojima I, Slayden RA. A Trisubstituted Benzimidazole Cell Division Inhibitor with Efficacy against Mycobacterium tuberculosis. PLoS One. 2014 Apr 15;9(4):e93953. doi: 10.1371/journal.pone.0093953.

Park B, Awasthi D, Chowdhury SR, Melief EH, Kumar K, Knudson SE, Slayden RA, Ojima I. Design, synthesis and evaluation of novel 2,5,6-trisubstituted benzimidazoles targeting FtsZ as antitubercular agents. Bioorg Med Chem. 2014 May 1;22(9):2602-12.

Pan P, Knudson SE, Bommineni GR, Li HJ, Lai CT, Liu N, Garcia-Diaz M, Simmerling C, Patil SS, Slayden RA, Tonge PJ. Time-Dependent Diaryl Ether Inhibitors of InhA: Structure-Activity Relationship Studies of Enzyme Inhibition, Antibacterial Activity, and in vivo Efficacy. ChemMedChem. 2014 Apr;9(4):776-91.

Schweizer HP, Limmathurotsakul D, Peacock SJ. New insights from the 7th world melioidosis congress 2013. Emerg Infect Dis. 2014 Jul;20(7). doi: 10.3201/eid2007.131737.

Delaney MA, Colegrove KM, Spraker TR, Zuerner RL, Galloway RL, Gulland FM. Isolation of Leptospira from a Phocid: Acute Renal Failure and Mortality from Leptospirosis in Rehabilitated Northern Elephant Seals (Mirounga angustirostris), California, USA. J Wildl Dis. 2014 May 7.

Spraker TR, Lyons ET, Kuzmina TA, Tift MS, Raverty S, Jaggi N, Crocker DE. Causes of death in preweaned northern elephant seal pups (Mirounga angustirostris, Gill, 1866), Ano Nuevo State Reserve, California, 2012. J Vet Diagn Invest. 2014 Mar;26(2):320-6.

Cautivo K, Schountz T, Acuña-Retamar M, Ferrés M, Torres-Pérez F. Rapid enzyme-linked immunosorbent assay for the detection of hantavirus-specific antibodies in divergent small mammals. Viruses. 2014 May 6;6(5):2028-37. doi: 10.3390/v6052028.

Schountz T, Prescott J. Hantavirus immunology of rodent reservoirs: current status and future directions. Viruses. 2014 Mar 14;6(3):1317-35.

Bobosha K, Tjon Kon Fat EM, van den Eeden SJ, Bekele Y, van der Ploeg-van Schip JJ, de Dood CJ, Dijkman K, Franken KL, Wilson L, Aseffa A, Spencer JS, Ottenhoff TH, Corstjens PL, Geluk A. Field-evaluation of a new lateral flow assay for detection of cellular and humoral immunity against Mycobacterium leprae. PLoS Negl Trop Dis. 2014 May 8;8(5):e2845. doi: 10.1371/journal.pntd.0002845.

Bian J, Kang HE, Telling GC. Quinacrine promotes replication and conformational mutation of chronic wasting disease prions. Proc Natl Acad Sci U S A. 2014 Apr 22;111(16):6028-33.

Lee JS, Bevins SN, Serieys LE, Vickers W, Logan KA, Aldredge M, Boydston EE, Lyren LM, McBride R, Roelke-Parker M, Pecon-Slattery J, Troyer JL, Riley SP, Boyce WM, Crooks KR, Vandewoude S. Evolution of puma lentivirus in bobcats (Lynx rufus) and mountain lions (Puma concolor) in North America. J Virol. 2014 Apr 16. [Epub ahead of print]

Jalkanen AL, Coleman SJ, Wilusz J. Determinants and implications of mRNA poly(A) tail size - Does this protein make my tail look big? Semin Cell Dev Biol. 2014 Jun 5. pii: S1084-9521(14)00173-6. doi: 10.1016/j.semcdb.2014.05.018.

Chapman EG, Costantino DA, Rabe JL, Moon SL, Wilusz J, Nix JC, Kieft JS. The structural basis of pathogenic subgenomic flavivirus RNA (sfRNA) production. Science. 2014 Apr 18;344(6181):307-10.

Chapman EG, Moon SL, Wilusz J, Kieft JS. RNA structures that resist degradation by Xrn1 produce a pathogenic Dengue virus RNA. Elife. 2014 Apr 1;3:e01892. doi: 10.7554/eLife.01892.

Walsh KP, Minamide LS, Kane SJ, Shaw AE, Brown DR, Pulford B, Zabel MD, Lambeth JD, Kuhn TB, Bamburg JR. Amyloid-β and Proinflammatory Cytokines Utilize a Prion Protein-Dependent Pathway to Activate NADPH Oxidase and Induce Cofilin-Actin Rods in Hippocampal Neurons. PLoS One. 2014 Apr 23;9(4):e95995. doi: 10.1371/journal.pone.0095995.


ASV Logo

MIP hosted a very large (~1,400 attendees) and very successful meeting of the American Society for Virology last month. The meeting boasted a record number of scientific workshops (56) along with morning plenary sessions, state-of-the art lectures, lunch time workshops special topic/discussion tables, poster sessions and a keynote address by Nobel laureate David Baltimore. With ~300 international attendees from ~40 countries, the meeting truly had a global flavor.

In addition to the outstanding science, the meeting attendees had a bit of fun as well. Two22 Brewery from Centennial developed and brewed a special craft beer for the occasion which we called ‘Hopatitis Ale’. Tuesday night entertainment in the social tent (before it was cut short by the violent hail storm in the area….) was provided by the terrific local alternative country rock band the Patti Fiasco.

Special thanks to Jeff Wilusz, the lead on site organizer of the conference, Olve Peersen (Biochemistry) who played a major role in the meeting (including website maintenance and AV coordination) and other members of the MIP faculty planning committee (Sandy Quackenbush, Joel Rovnak, Brian Geiss, Tony Shountz, Rushika Perera, Carol Blair, Greg Ebel and Ken Olson) whose tireless and effective efforts were key to making this one of the best ASV meetings ever. The real key, however, to pulling off this meeting though were the 65 amazing, hard-working student/postdoc/RA/RS/admin coordinator volunteers that truly made everything work as planned. Working with these guys is a true honor.

A big thank you also goes out to CSU entities that worked the event. These include Elodie Vigneron and Conf Services, CVMBS IT (including Erin Napier who originally designed the terrific ASV2014 website), LSC Catering, Jason Rogien and the LSC AV crew, Allen Sneesby and Classroom Services, Housing and Dining, TILT staff, CSU Warehouse Staff, CSUPD, CSU motor pool, and countless others who worked behind the scenes.

Lastly we’d like to thank all of our local sponsors that truly helped make the meeting special. These include the Hilton Hotel who did an amazing job hosting the plenary sessions and the banquet, and several local breweries - Black Bottle, Two22 and Fort Collins Brewery - for providing beverages for the social tent (the quality of which attendees are still raving about). There is little doubt that meeting attendees left Northern Colorado with a terrific impression of our fair city and its top-notch businesses.

Anyone crazy enough to do this again at CSU in ~6 years?????????

ASV 2014 Patti Fiasco concert

The dance floor in the social tent is packed as ASV2014 attendees enjoy the Patti Fiasco concert/dance on the last night of the meeting.

ASV 2014 Hopatitis Ale LogoASV 2014 Hopatitis Ale Pint Glass

Hopatitis Ale was designed and brewed specifically for ASV2014 by Two22 Brewery in Centennial. Special thanks to our own Ashley Malmlov for designing the logo. Every attendee received a 'Hopatitis Ale' pint glass as part of the meeting swag package.

Happy Retirement

Barry Beaty Pat Brennan

A celebration to honor Dr. Barry Beaty and Dr. Patrick Brennan was held on April 2, 2014 at the Foothills Research Innovation Center. Dr. Beaty and Dr. Brennan were recognized for their outstanding achievements and exemplary service to CSU. We hope they are both enjoying their partial retirement!

Check out the photo gallery from the event.

Dodgeball Logo FAC Dodgeball 2014

In April, the Student Chapter of the American Veterinary Medical Association (SCAVMA) hosted the first annual FAC dodgeball tournament. The stakes were high as teams competed for best costume and the coveted "Champion of the Universe" trophy. In the end the "Bottom Quartile" defeated "The Sitting Ducks" to take home the trophy and "Prognosis: Grave" won for best costume. The tournament also settled an ancient rivalry between pathology and surgery. In what was called the "Balls of Truth Showdown" EJ Ehrhart's team,"Large Knives", defeated Felix Duerr's team, "Small Knives".

Hey DJ

DJ Andy Bender

Andy and his co-host Jacqui Maes

Micro major, Andy Bender loves science, but he has another passion too. Andy is a DJ for KCSU, Colorado State University’s non-commercial, student-run, campus radio station. KCSU focuses on college and alternative musical genres but also provides newscasts, sports updates, a public affairs talk show and Public Service Announcements. Right now Andy and his co-host, Jacqui Maes, have a two hour show from 11:00 pm to 1:00 am where they play "relaxed electronic/study music". They are hoping to get a more accessible time slot over the summer. Check out the live stream and tune into Andy during those late night study sessions!

Graduate Recruitment Snow Shoeing Grads 014

Thirteen prospective MIP graduate students visited the CSU campus in February; one candidate visited on Monday, Feb 24th, and twelve visited on Friday, Feb 28nd. Both groups were given an overview of the Graduate program from Dr. Quackenbush, received tours of the Foothills facilities, met with the GEC, and met with individual faculty whose research programs were of interest to them. The students who visited on Friday were able to attend the CMB/MCIN/BMB/MIP Poster Symposium and then enjoyed a FAC with faculty and the Graduate Student Organization at Tap & Handle. Saturday morning, due to weather conditions, and after scraping ice off the vehicle windows they headed to Lory State Park to hike Arthur's Rock with Drs. Blair, Black, Quackenbush, Rovnak and Schountz. View more photos from the hike.

Micro Major, Samantha Guinn, wins the 2014-15 ASCSU Presidential Election

Guinn and Li Puma ASCSU election

Samantha and her running mate Lance Li Puma

Samantha Guinn, a junior microbiolgoy major is the new president of ASCSU. Samantha and her running mate, Lance Li Puma, beat out three other campaigns with 1,484 votes. Their campaign "Fresh Approach, New Beginnings, focused on four separate platforms. Congratulations Samantha and Lance!

Read the Collegian article for the full story.

Career Workshop
MIP student Career Fair

In April, MIP students enrolled in the Plan B masters and PhD programs had the opportunity to attend a career workshop. The event was held in the D-lab atrium of the South campus and featured four invited speakers. Rob Yemm from Leprino Foods; Dan Stinchcomb, founder of Inviragen; Ann Powers from the CDC and Dennis Pierro, Director of BioMARC. The goal was to highlight possible career choices in microbiology/biotech/food industry. Each of the speakers gave a short presentation about careers in their areas, and also talked about their own career experiences to help the students. The presentations were followed by a panel discussion based on the students' questions.

Spring Picnic

Good food, good beer and good people were all part of the MIP Annual Spring Picnic on Friday, May 9. MIPers and their families enjoyed Famous Daves BBQ while Greg Dean and Becky Trentlage poured the beer.

Thank you to Ida Tieman, Cathy Griffin, Andrea Franson and Becky Trentlage for organizing the event, and to the MSA and MSO students for their labor and financial help in making the picnic a success!

Check out the Spring Picnic Photo Gallery - complete with goofy captions.

Making a Difference Megan Cassidy

Megan Cassidy, an MIP alumni, was recently chosen for the AP Biology Leadership Academy. She is currently a teacher at Horizons High School in Thornton, CO and judging from the letter below, she is already making a difference. Congratulations Megan!

Megan Cassidy Letter

Leprosy Research in Brazil Leprosy Documentary

Dr. John Spencer is part of a documentary featuring his research diagnosing leprosy in Breves, Brazil. The documentary was produced with funding from the Brazil Ministry of Health and shadows a team as they vist homes and schools in the Amazon of Brazil. Be sure to check out the video on youtube!

Funding for Publication Costs

Image of Journal articles

The Colorado State University Libraries has allocated $45,000 to establish the Open Access Research & Scholarship (OARS) Fund. CSU tenured/tenure track faculty authors are eligible to receive up to $2,000 to support article processing fees for one article. The 2013-2014 academic year application period for OARS funding closed May 31, however, the website indicates OARS will be accepting applications again in mid-July. Please email michelle.wilde@colostate.edu if you would like to be notified when the program begins accepting applications again.

News Update

The video Jordon Steel submitted for the ASM Global Video Challenge - People's Choice Award tied for 3rd place. Jordan's video answered the question how is your microbiology making the world a better place? Jordan will receive a $100 cash prize and free registration to the ASM general meeting. His video was honored at the State of the Society Address on Monday, May 19. Congratulations Jordan!

MIP Bits

Quote of the Month

"As amazing as it sounds, the ball hit my mustache, It was a mustache goal. "
  ~ Frederico Haves Guedes, Brazilian soccer player


You can oftentimes find some unexpected visitors on our campus!

    Alternative Captions:
  1. Since when did Geico start sponsoring the MIPnews?
  2. One sure sign that global warming has hit northern Colorado
  3. If someone tells you that you’re as photogenic as a camel, take it as a compliment
  4. Anyone up for a new mascot to go along with a new stadium? Could probably still call him ‘Cam’…

Questions for MIPuzzle #92
MIPuzzle #92 Answers
MIPuzzle #92

Keep Calm Holiday

2014-2015 Fiscal Year Holiday schedule

Friday, July 4
Monday, September 1
Thursday, November 27
Friday, November 28
Wednesday, December 24
Thursday, December 25
Friday, December 26
Thursday, January 1
Monday, January 19
Monday, May 25

John Belisle

John shows how tall he used to be before he took over the RM-RCE and the weight on his shoulders took its toll….

    Alternative Captions:
  1. Unsure how to use this new fangled ‘whiteboard app’, Dr. Belisle resorts to charades to make a point in Microbial Physiology
  2. Fearing a grizzly gear walked into his classroom, John tries to make himself look large. Turns out it was just Bill Black before he had his morning coffee.
  3. Submit your own

NIH Happenings

  • New Online CV Tool

    Check out the new Science Experts Network Curriculum Vitae (SciENcv) tool that’s currently in test phase. This software lets you create an online CV that you can share with others- and most importantly- generate a biosketch for your grant application.

  • PubMed is Becoming More Interactive

    NIH is opening up the door to more scientific discourse by creating PubMed Commons – a system that allows authors to discuss and share information through comments on article citations. Check it out at http://www.ncbi.nlm.nih.gov/pubmedcommons

New Grant Awards

bulletJohn Belisle, "Mycolic Acid Assay Development and Testing", Catalysis Foundation

bulletLars Eisen, "Novel Trap for Ticks and Fleas Incorporating a CO2 Generator", TDA Research, Inc.

bulletAngelo Izzo, "Treatment of Irradiated M. Tuberculosis Strains for Use as Vaccines", Mico Bio, Inc.

bulletHerbert Schweizer, "ALX-009 Efficacy Testing Against Priority Pathogens", Alaxia

bulletSchweizer, Herbert, "Biapenem/RPX7009 Activity Against Burkholderia Pseudomallei", Rempex Pharmaceuticals

bulletMathiason, Candance, "Detection and Characterization of Blood-borne Prions", NIH-NIAID

bulletBeaty, Barry, "Metabolomics-based Discovery of Small Molecule Biomarkers for Noninvasive Dengue Diagnosis and Prognosis", NIH-NIAID

bulletDobos-Karen, "TB Surrogate Markers for Assessing Response to Treatment (TB SMART Study)", University of California, San Francisco

JULY 2014
Mon Tue Wed Thur Fri
American flagIndependence Day
Univ Offices Closed
7 8
9 10 11

Mon Tue Wed Thur Fri

4 5
6 7 8
Fall Classes Begin

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Send In your ideas or newsworthy items. Contributions make the Newsletter better!

MIP Newsletter Volume 11, Issue 3, July 2014

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