Volume 9, Issue 1, January 2012
Dr. Jim Kennedy, Director of the Veterinary Diagnostic Laboratory at the Rocky Ford Branch, unexpectedly passed away on December 31, 2011. In addition to his strong leadership he was an outstanding veterinarian, diagnostician, colleague and friend. Jim grew up in Kansas and earned his veterinary degree in 1979 at the University of Missouri . Prior to obtaining his DVM, he served as Captain in the US Air Force between 1970 and 1974. He began his veterinary career in production animal medicine, working in cow-calf operations, first in South Dakota and then for many years in Kansas. He then turned his attention to academia and taught at the University of Nebraska before arriving at the CSU VDL in September 2002. In his ten years as Director, he expanded the services of the laboratory and increased the volume of use of the laboratory five fold. Jim was dedicated to serving all clients of the laboratory and his focus was on improving and providing practical diagnostics for the benefit of cattle and cattle producers. He was a pioneer in developing pooling techniques for Bovine Viral Diarrhea (BVD) and Trichomoniasis. He vigorously evaluated all aspects of pooling technology, not only on the test sensitivity and specificity, but also the practical application of these diagnostic strategies in the field and how they impact the producer, improve herd health and consequently the economics of the cattle industry. He was a leader in developing a BVD voluntary Control Program in the State of Colorado which soon became a model for other states that adopted similar programs. This program involves not only testing cattle for BVD but also in developing good biosecurity strategies to prevent disease from entering herds. Similarly, Jim developed and improved testing strategies for Trichomoniasis and was the lead scientific advisor to the state's bovine trichomoniasis advisory panel. In 2007 Jim was recognized for his many professional contributions as Colorado Veterinarian of the Year by the Colorado Veterinary Medical Association. He was a regular contributor to the CSU laboratories newsletter, LabLines, providing useful real-world information for livestock veterinarians and producers. In addition he published his scientific findings in peer-reviewed veterinary journals. He was a prolific public speaker within the state, western region and across the country. He was a regular participant in the Academy of Veterinary Consultants and was the liaison between the Academy and the American Association of Veterinary Laboratory Diagnosticians.
Beyond his administrative and scientific accomplishments, Jim was a wonderful person that many across the country claimed as friend. Jim's "very dry" sense of humor kept those around him amused and guessing whether he was serious or teasing. Jim was a recognized and respected member in his community of Rocky Ford, actively participating in the Rotary Club and his church. Jim loved flying; he had his own small plane and pilots license. He frequently would fly over the Rocky Ford area and wave his wings at his neighbors. He felt that flying gave him a different perspective on the world and made all problems disappear. After spending nearly a decade with us at the Diagnostic Laboratory, Jim was planning to retire in 2012. We are saddened that he will not get a chance to enjoy retired life following a very successful career. He will be missed by all of us in the Diagnostic Laboratory. He will be missed by many across the state, especially in Southern Colorado. To members of the Department of Microbiology, Immunology and Pathology – Many of you did not have the pleasure of knowing Jim Kennedy as he resided in the distant portion of Southern Colorado in the Rocky Ford Laboratory. He was the face of the MIP Department and the College, as well as University in that part of the state. He brought considerable recognition to the University through his work with the cattle ranchers and veterinarians. He was the model of providing quality service to the public and was a highly respected member of the community. Jim made very meaningful, long-lasting and significant advances in the practice of diagnostic veterinary medicine and always will be remembered for his valuable contributions. It will be very difficult to find a replacement of his caliber.
Each semester the MIP faculty recognize an outstanding graduating senior for academic excellence and/or service to the department or the community. Megan Vogt was the recipient of this award for the December 2011 graduating class.
Megan is truly an outstanding student, graduating summa cum laude with a perfect GPA of 4.0! Megan earned these exemplary grades completing not only the rigorous curriculum required for a B.S. degree in microbiology, but also for completion of the Honors Scholars Program and a minor in French.
In addition to being a full-time student, Megan also worked for several years in the research laboratory of Dr. Sandy Quackenbush, where she was assigned a very difficult project studying retroviral disease in fish. This experience has honed Megan into quite a fine young researcher and she is already a co-author on an article published in the Journal of Virology, which is certainly a major accomplishment for any undergraduate.
Although Megan is a serious student and researcher, she has other interests as well. In addition to pursuing a minor in French, she loves poetry, plays the clarinet, is a peer tutor, and is a member of the national service fraternity Alpha Phi Omega, through which she volunteers to visit seniors living in rest homes, an activity she especially enjoys. Even those of you who know Megan may be completely unaware of all that Megan does, because she is a very modest young woman who rarely speaks of her accomplishments unless directly asked.
Megan intends to attend graduate school, and the question is not if she will be admitted, but rather which offer she will accept. Not surprisingly, Megan is interested in many areas of research and is taking time to decide which one she wants to pursue. In the interim, Megan has accepted a position as a research associate with Dr. Torsten Eckstein, where she will study bacteria instead of viruses, and she will also be a graduate teaching assistant for one of the undergraduate laboratory courses.
Congratulations, Megan, and good luck in your future endeavors!
Graduates who attended the Graduation Ceremony on December 17th
GT Gorbold, Megan Vogt, Yasmine Salas, Rachel Schauer, Laura Kazee, Gwen Elder, Lauren Cope
We thought it would be a good idea to highlight some of the outstanding work that is performed by our undergraduates doing research in laboratories at part of their MIP 298/498 coursework. Every semester, students are required to turn in a written report of their laboratory experience - here's one that has been chosen from the Fall 2011 Semester. Congratulations to Jason Christianson on a job well done! "The effect of OKA on SinV replication"
Stable Extracellular RNA Fragments of Mycobacterium tuberculosis Induce Early Apoptosis in Human Monocytes via a Caspase-8 Dependent Mechanism
Andres Obregon-Henao, Maria Duque-Correa, Mauricio Rojas, Luis Garcia, Pat Brennan, Blanca Ortiz and John Belisle
PLos ONE, Vol. 7, Issue 1, e29970, January 2012
Did you ever wonder whether cells, after spending >80% of their metabolic energy on the synthesis of ribosomal and tRNA, might consider getting their money’s worth and use these molecules for something else in addition to translation? Of course you didn’t! The central dogma of gene expression is so engrained in our thinking that tRNAs and rRNAs could only be used for protein synthesis – heck we learn that in Biology 101. Cell biology couldn’t be more complicated than that, right? Wrong (accompanied by a loud ear piercing buzzer)!
While investigating factors secreted by M. tb that cause programmed cell death (apoptosis) in macrophages, Andres et al have made a rather startling discovery. Purifying the M. tb ‘death’ factor from culture filtrates through a DEAE column and acrylamide gels, as well as treating it RNases, have all pointed the finger at mycobacterial tRNA and rRNA fragments as effective mediators of apoptosis. The small M. tb RNAs appear to act through a potentially novel caspase-8 pathway. The tRNA and rRNA fragments are likely released by living bacteria and also make macrophages more susceptible to M. tb colonization. While a small handful of previous papers have suggested potential roles for RNA fragments in mycobacteria biology, this work from the Belisle group points to some rather novel mechanisms. Interestingly, rRNA and tRNA fragments, as well as tRNA-like molecules, are beginning to gain some attention in cancerous cell growth and as regulators and signalers in cellular stress responses (reviewed in Wiley Online Library ). Thus perhaps it’s not surprising that mycobacteria want to get into the small RNA act. One has to wonder if other bacteria use this strategy as well.
So why did we pick this paper as our coveted MIPublication of the Month® to kick off the Year of the Dragon? As usual, we have three main reasons. First, this is a rather novel bit of science that brings together some previously unrelated areas of cell biology and may open up some very interesting new avenues for research. Second, the Belisle lab has three papers on our ‘Pub’ list this month – which we believe may tie a record. Surely that feat deserves a highlight in and of itself. Finally, it’s taken John half a century or so, but we think he might have ‘grown up’. Do you think he’s ready to drop his previous ‘sugar and fat’-only scientific life style and come around to that really cool, cutting-edge RNA-way of thinking? Only time and the amount of inane jokes that he utters will tell…
Carlsten KS, London CA, Haney S, Burnett R, Avery AC, Thamm DH. Multicenter prospective trial of hypofractionated radiation treatment, toceranib, and prednisone for measurable canine mast cell tumors. J Vet Intern Med. 2012 Jan;26(1):135-41.
Parlane NA, Compton BJ, Hayman CM, Painter GF, Basaraba RJ, Heiser A, Buddle BM. Phosphatidylinositol di-mannoside and derivates modulate the immune response to and efficacy of a tuberculosis protein vaccine against Mycobacterium bovis infection. Vaccine. 2012 Jan 11;30(3):580-8.
Parlane NA, Grage K, Mifune J, Basaraba RJ, Wedlock DN, Rehm BH, Buddle BM. Vaccines Displaying Mycobacterial Proteins on Biopolyester Beads Stimulate Cellular Immunity and Induce Protection against Tuberculosis. Clin Vaccine Immunol. 2012 Jan;19(1):37-44.
Machain-Williams C, Mammen MP Jr, Zeidner NS, Beaty BJ, Prenni JE, Nisalak A, Blair CD. Association of human immune response to Aedes aegypti salivary proteins with dengue disease severity. Parasite Immunol. 2012 Jan;34(1):15-22.
Obregón-Henao A, Duque-Correa MA, Rojas M, García LF, Brennan PJ, Ortiz BL, Belisle JT. Stable Extracellular RNA Fragments of Mycobacterium tuberculosis Induce Early Apoptosis in Human Monocytes via a Caspase-8 Dependent Mechanism. PLoS One. 2012;7(1):e29970.
Bhamidi S, Shi L, Chatterjee D, Belisle JT, Crick DC, McNeil MR. A bioanalytical method to determine the cell wall composition of Mycobacterium tuberculosis grown in vivo. Anal Biochem. 2012 Feb 1;421(1):240-9.
Mahapatra S, Woolhiser LK, Lenaerts AJ, Johnson JL, Eisenach KD, Joloba ML, Boom WH, Belisle JT. A Novel Metabolite of Antituberculosis Therapy Demonstrates Host Activation of Isoniazid and Formation of the Isoniazid-NAD+ Adduct. Antimicrob Agents Chemother. 2012 Jan;56(1):28-35.
Bessen RA, Robinson CJ, Seelig DM, Watschke CP, Lowe D, Shearin H, Martinka S, Babcock AM. Transmission of chronic wasting disease identifies a prion strain causing cachexia and heart infection in hamsters. PLoS One. 2011;6(12):e28026.
Bessen RA, Wilham JM, Lowe D, Watschke CP, Shearin H, Martinka S, Caughey B, Wiley JA. Accelerated Shedding of Prions following Damage to the Olfactory Epithelium. J Virol. 2012 Feb;86(3):1777-88.
Reisfeld B, Metzler CP, Lyons MA, Mayeno AN, Brooks EJ, Degroote MA. A physiologically based pharmacokinetic model for capreomycin. Antimicrob Agents Chemother. 2012 Feb;56(2):926-34.
Mehaffy MC, Kruh-Garcia NA, Dobos KM. Prospective on Mycobacterium tuberculosis Proteomics. J Proteome Res. 2012 Jan 1;11(1):17-25.
Jaenson TG, Jaenson DG, Eisen L, Petersson E, Lindgren E. Changes in the geographical distribution and abundance of the tick Ixodes ricinus during the past 30 years in Sweden. Parasit Vectors. 2012 Jan 10;5(1):8.
Shoeneman JK, Ehrhart EJ 3rd, Eickhoff JC, Charles JB, Powers BE, Thamm DH. Expression and function of survivin in canine osteosarcoma. Cancer Res. 2012 Jan 1;72(1):249-59.
Perrott MR, Sigurdson CJ, Mason GL, Hoover EA. Evidence for distinct chronic wasting disease (CWD) strains in experimental CWD in ferrets. J Gen Virol. 2012 Jan;93(Pt 1):212-21.
Wang Y, Hess TN, Jones V, Zhou JZ, McNeil MR, Andrew McCammon J. Novel inhibitors of Mycobacterium tuberculosis dTDP-6-deoxy-L-lyxo-4-hexulose reductase (RmlD) identified by virtual screening. Bioorg Med Chem Lett. 2011 Dec 1;21(23):7064-7.
De Groote MA, Gruppo V, Woolhiser LK, Orme IM, Gilliland JC, Lenaerts AJ. Importance of Confirming Data on the In Vivo Efficacy of Novel Antibacterial Drug Regimens against Various Strains of Mycobacterium tuberculosis. Antimicrob Agents Chemother. 2012 Feb;56(2):731-8.
Li X, Liu N, Zhang H, Knudson SE, Li HJ, Lai CT, Simmerling C, Slayden RA, Tonge PJ. CoA Adducts of 4-Oxo-4-Phenylbut-2-enoates: Inhibitors of MenB from the M. tuberculosis Menaquinone Biosynthesis Pathway. ACS Med Chem Lett. 2011 Nov 10;2(11):818-823
Chantratita N, Tandhavanant S, Wikraiphat C, Trunck LA, Rholl DA, Thanwisai A, Saiprom N, Limmathurotsakul D, Korbsrisate S, Day NP, Schweizer HP, Peacock SJ. Proteomic analysis of colony morphology variants of Burkholderia pseudomallei defines a role for the arginine deiminase system in bacterial survival. J Proteomics. 2012 Jan 4;75(3):1031-42.
Bielefeldt-Ohmann H, Smirnova NP, Tolnay AE, Webb BT, Antoniazzi AQ, van Campen H, Hansen TR. Neuro-invasion by a 'Trojan Horse' strategy and vasculopathy during intrauterine flavivirus infection. Int J Exp Pathol. 2012 Feb;93(1):24-33.
Bellofatto V, Wilusz J. Transcription and mRNA stability: parental guidance suggested. Cell. 2011 Dec 23;147(7):1438-9.
Miller DS, Weiser GC, Aune K, Roeder B, Atkinson M, Anderson N, Roffe TJ, Keating KA, Chapman PL, Kimberling C, Rhyan J, Clarke PR. Shared Bacterial and Viral Respiratory Agents in Bighorn Sheep (Ovis canadensis), Domestic Sheep (Ovis aries), and Goats (Capra hircus) in Montana. Vet Med Int. 2011;2011:162520.
Gee CL, Papavinasasundaram KG, Blair SR, Baer CE, Falick AM, King DS, Griffin JE, Venghatakrishnan H, Zukauskas A, Wei JR, Dhiman RK, Crick DC, Rubin EJ, Sassetti CM, Alber T. A phosphorylated pseudokinase complex controls cell wall synthesis in mycobacteria. Sci Signal. 2012 Jan 24;5(208):ra7.
Geluk, A., M. S. Duthie, and J. S. Spencer. 2011. Postgenomic Mycobacterium leprae antigens for cellular and serological diagnosis of M. leprae exposure, infection and leprosy disease. Lepr. Rev. 82:1-20.
Spencer, J. S. and P. J. Brennan. 2011. The role of Mycobacterium leprae phenolic glycolipid I (PGL-I) in serodiagnosis and in the pathogenesis of leprosy. Lepr. Rev. 82:340-353.
MIP ROADTRIP to see the Rockies
With Tebow-time over, its time for all Colorado sports fans to start thinking Tulo-time! MIP has reserved a block of field level box seats to a Rockies game once again this year. The tickets are along the first base line in sections 116/ 117 to see the Colorado Rockies take on Albert Pujols and the LA Angels in interleague action on Friday, June 8th at 6:40PM at Coors Field in Denver. The price per ticket is $23 - an almost 50% savings over the face value.
We are only taking ticket reservations now - no need to come up with any cash until ~ April. All members of the extended MIP family are invited - so bring the spouse, bring the kiddies, bring the kiddies' spouses, etc. Tickets are going fast - so please email Jeff Wilusz ASAP if you'd like to reserve some tickets to this 'can't-be-missed' Departmental outing.
Above is a picture of all of the wonderful gifts that we collected throughout the MIP Dept for the Angels Among Us 2011 Holiday Campaign. Congratulations and big THANK YOU to Karen Hofmaier, Tach Costello, Cathy Griffin and Carol Wilusz who helped coordinate the donations.
The 2011 Pine Ridge Indian Reservation/Dell Big Crow Holiday Gift Project was once again a huge success! We enlisted the help of Pine Ridge community organizers, teachers, and trusted friends who provided us with wish lists for elders and children with the highest needs on the reservation. This year we were very pleased to provide assistance to the Homeless Veteran's Shelter on Pine Ridge, also. Because of your generosity nearly 1000 people on the Pine Ridge Indian Reservation were provided with a little holiday cheer.
While it saddened us once again to see how many people were in need of basics such as shoes, socks, clothing, and warm blankets, we were made aware of how excited the people were when they heard the UPS truck coming. One of the elders told us that her husband was so excited when the packages arrived that "he tore them all open right then - couldn't even wait for Christmas! Just like a little child!" Another elder told us "Thank everyone for all the things they sent to our families and neighbors and our children and grandchildren. Sometimes we think no one cares about us, but we are so thrilled to know that there are so many wonderful people in the world who remember us and think about us and it makes us feel happy and important. Everyone had a good Christmas and the kids were thrilled."
We simply do not have the words to thank you enough for your many, many kindnesses…we just hope you know in your hearts that you changed the lives of many deserving people and made their wishes come true. A special thanks to all of you who passed the information about this project on to your friends and relatives…this helps the project grow and grow! Wopila Tanka!
~Chris Bartholomew and Julie Sullivan
Enjoy and evening of ballroom, latin and swing dancing on February 11 with the Foothills Pops and Swing Band. MIP's very own Glenda Allen plays the alto saxophone in the Band. See the attached flyer for more information.
Application Deadline is
The ASM Undergraduate Research Fellowship (URF) is aimed at highly competitive students who wish to pursue graduate careers (Ph.D. or MD/Ph.D) in microbiology. Students will have the opportunity to conduct full time research at their home institutions with an ASM member and present research results at the ASM General Meeting the following year.
See the ASM Undergraduate Research Fellowship Website for more information
Deadline: March 19 at 5PM
Several changes thave been made to the CRC process this year. It is strongly suggested you review the FY13 CRC proposal instructions carefully.
CMB/MCIN/BMB/MIP Poster Symposium
Save the Date!
It is time again for food, fun, drink and a little competition. Please consider presenting a poster for the Annual Cell & Molecular Biology/Molecular, Cellular & Integrative Neurosciences/Biochemistry & Molecular Biology & Microbiology, Immunology & Pathology poster symposium. Presenters can either be graduate students or postdocs and are welcome to use posters presented at other symposiums. This is also graduate recruitment weekend for these programs.
See the abstract submission flyer for more information. Submission Deadline is February 10th!
MIPers and Microbiologists of all Ages - MARK YOUR CALENDARS
The Spring meeting of the Rocky Mtn Branch of the American Society for Microbiology will take place on April 20th (Fri evening plenary talk) and 21st (full day Saturday 9-5PM talk and poster sessions). This is a fantastic opportunity (aka supportive, high yield environment) for grad students, postdocs and undergrads to present their research projects in a poster or oral format. As the premier microbiology department in the state, I think that MIP should have a strong showing at this meeting. I hope that you agree.
Here are the specifics:
When: April 20th and 21st (all student/postdoc talks will take place on Saturday 4/21; its OK to just come for the Saturday session)
Where: The University of Denver
Registration deadline: TBA
Saturday Plenary Speaker: Dr. Yousif Shamoo, Rice University ‘Experimental Evolution as a Tool for Completing the Link Between Genomics, Biochemistry and Prediction in Antibiotic Resistance’
Celebrate the accomplishments of undergraduate researchers at the Celebrate Undergraduate Research and Creativity Symposium being held this year on April 17 with an awards ceremony on Thursday, April 19.
Last day to register: March 23, 2012 at Noon
Visit the CURC Website for more information.
The MRL held their yearly potluck and cooking contest which included a Sushi Chef and attendees voting for best appetizer, main dish, and dessert!
Want to do more in the battle against TB?
Asma Henry (our prize winning curried potato chef at the MRL Holiday Party) helps to run a tuberculosis patient organization in the Republic of Moldova, a tiny country located between Romania and Ukraine. Speranta Terrei raises awareness of TB, gives treatment adherence support to TB patients, and promotes their rights and duties. Community members, called moderators, take TB drugs from the dispensary to patients' homes and observe ingestion. The home visits are more than a medical exercise because moderators may counsel patients on reducing alcohol intake or assist them to find temporary housing. They break the isolation and psychological barriers of an infectious disease. Their encounters with patients are unhurried and they discuss changes in the drug regimen as well as in local politics.
Speranta Terrei won the 2008 Tuberculosis Survival Prize from the TBSP, but needs additional funds to support its work. Please see Speranta Terrei's project on Global Giving to raise funds for treatment support.
Asma promises a generous portion of her prize-winning potato curry to all contributors!
Congratulations to former DMIP PhD student, Tim Kurt, and his wife Liz on the recent birth of their son, Asher. The photo above shows Asher at approx 2 mos old reviewing a black and white photo of a well-racked deer.
On a recent visit to Coors Field for Rockies FanFest, a roving MIPnews reporter had the opportunity to tour the field level facilities used by the team. While checking out the umpire's lounge, we discovered something a bit curious. The next time that you question the judgment of an umpire on the field, please bear in mind that the sign on the umpire's lounge door at Coor's Field is also written in Braille...
On a visit this Fall to a local Safeway supermarket, a roving MIPnews contributor noted this interesting item that is on sale for ‘Club Hebrew Nation’. Yes folks, your eyes aren't deceiving you, it is indeed pork at half price.
New Grant Awards
Anne Lenaerts, "The Mycobacterium Tuberculosis Dormancy Program", Univ of Colorado
Anne Lenaerts, "Assessment of Liposomal Drugs Alone and in Combination for TB Efficacy", Merrimack Pharmaceuticals, Inc.
Delphi Chatterjee, "Modified DNA Aptamer Affinity Reagents for Mtb Biomarker Validation", Univ of Colorado
Herbert Schweizer, "In Vitro and In Vivo Compound Testing ", Evolva, Inc.
Do you have NEWS or PICTURES you would like to share?
Send In your ideas or newsworthy items. Contributions make the Newsletter better!
|MIP Newsletter Volume 9, Issue 1, January 2012|
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