Vol. 10 | No. 7
Congratulations to Dr. Patricia Cole for receiving the 2013 CVMA Outstanding Faculty Award for her selfless contributions and support for her students. She is known as a gifted instructor among students and colleagues, carrying the title of veterinarian with pride and encouraging her students to do the same. Please note that MIPnews respectfully requests that even though she's on top of the veterinary world right now and clearly 'unforgettable', please refrain from referring to her as 'Pat 'King' Cole'.....
The Annual American College of Veterinary Pathologist (ACVP) Board Exam was held September 17-19. Great job to all! Passing any or all is an accomplishment and we're proud of your efforts and success!
Dr. Amy MacNeill signs on the
Dr. Amy MacNeill (no relation to recently retired MRL professor Mike...) from the University of Illinois has accepted an Associate Professor Faculty position in Clinical Pathology. She will be joining MIP in January 2014.
Congratulations to Jonathan Seaman, undergraduate in the Brian Foy Laboratory, for recieving a 2013 ASM Undergraduate Research Fellowship. Jonathan will be conducting his summer research project entitled, "The Effect of Senescence on Ivermectin Susceptibility in Malaria Transmitting Mosquitoes" and have the opportunity to present his results at the 114th ASM General Meeting in Boston, MA.
This year there were 138 applications and Jonathan was one of 37 who received the award. This fellowship is aimed at highly competitive students who wish to pursue graduate careers in microbiology.
Randy Basaraba's work 'laying down the blacktop' towards understanding the connection between diabetes and tuberculosis was recently featured in the American Diabetes Association Research Highlights 'A Radical New Road for Research' brochure.
Congratulations to Dr. Seung Yoo for receiving the CL Davis Student Scholarship Award this year for his accomplishments in superior scholarship, leadership, research ability and diagnostic skills. Seung will receive his award at the 64th Annual Meeting of the American College of Veterinary Pathologists, November 16-20 in Montreal.
The MRL is pleased to announce that Dr. Francisco Olea-Popelka is joining the MRL. Dr. Olea-Popelka is a DVM and holds a PhD degree in epidemiology. He is currently a Faculty in the Dept. of Clinical Sciences. His research focuses on M. bovis and the transmission of TB from animals to animals, and animals to humans. He is involved in multiple field studies in Europe, Africa, South America and Asia and is already collaborating with the MRL. Dr. Olea-Popelka is an enthusiastic young researcher and interested in collaborating with the MRL on various aspects of mycobacterial disease transmission, drug resistance and the global epidemiology of mycobacteria.
Congratulations to Rick Barz who received the College of Veterinary Medicine and Biomedical Sciences 2013 Honor Alumnus Award. Rick is a 1975 graduate of our microbiology program. He started as a technician with Leprino Foods and recently retired as vice president of product quality and development.
Microbiology senior, Guy Stewart and his team presented their work on the creation of a biofilm that self inflates a bike tire at the 2013 iGEM competition in Toronto. The iGEM team was able to complete their project with the generous support of the MIP department and CVMBS.
Correlates of Immune Protection Following Cutaneous Immunization with an Attenuated Burkholderia pseudomallei Vaccine
Ediane Silva, Andrew Goodyear, Marjorie Sutherland, Nicole Podnecky, Mercedes Gonzalez-Juarrero, Herbert Schweizer and Steve Dow
Infection and Immunity, published online October 7, 2013
Want to know what’s worse than the smell of roofing tar on a warm autumn afternoon compounded by the incessant hammering of a wrecking crew dismantling a fifty year old freezer/coldroom down the hallway? Melioidosis caused by Burkholderia pseudomallei. Breathe in this little gram negative bacterial rod (instead of those wonderful sulfer compounds in roofing tar) and you may very well be in for a bout of pain, pneumonia and widespread abscesses that is oftentimes fatal without treatment (and if it doesn’t get you the first time around, the bacteria can cause chronic infections that crop up again down the road). To make matters worse, efflux pumps in the bug make it inherently resistant to many antibiotics and there is no vaccine. Given the prevalence of this pest in Southeast Asia, Northern Australia and parts of South and Central America – not to mention the fact that sensitive methods are detecting it in novel places around the globe – we could really use a vaccine against this germ.
Ediane et al analyzed whether or not an attenuated ΔpurM derivative of the Bp82 strain of B. pseudomallei might be a good candidate for a vaccine when delivered by the sub-cutaneous route. This Bp82 strain is so weakened that it has never been observed to revert to virulence and its actually been approved for experiments at the BSL2 level in standard labs (rather than restricted to study behind the BSL3 barrier in our Regional Biocontainment Lab like all other B. pseudomallei strains). Following green fluorescent protein-labeled bacteria in lymph nodes during infection, it was clear the mouse reacts with a rather novel strong neutrophilic response to Bp82 along with antigen presenting cells. Two vaccinations with this Bp82 strain fully protected C57BL/6 mice from a normally deadly intranasal challenge with virulent B. pseudomallei. Instead of finding ~a million bacteria in the liver of infected animals as expected, vaccinated critters had zero, zilch, nada. Impressed yet? Well you know those MIP immunologists weren’t just going to stop there – they wanted to learn how the vaccine worked. Vaccinated animals showed a very strong IgG and IgM response against the bugs, with antibodies being primarily targeted to bacterial surface proteins. Based on three experiments, this humoral antibody response appeared to play a very important role in saving the vaccinated mice from certain death. Transfer of serum to naïve animals saved 38% from succumbing to the disease. Next, vaccinated CD4 and CD8 knockout mice were surprisingly protected from the disease, suggesting that T cell-independent antibodies play a key role. Finally, B cell deficient mice were significantly less well protected by vaccination than their wild type cage mates.
So why did we pick this paper for our coveted MIPublication of the Month®? First, the study implies that a safe and effective vaccine that can be delivered by a sub-cutaneous route may very well be in the cards for melioidosis in the not too distant future. That’s great news, particularly for those in endemic regions of the globe as well as folks trying to prop up the biodefense infrastructure of our country. Second, the study is a terrific example of that good olde MIP synergy with three separate labs coming together to share expertise to contribute to this fine piece of work. Finally, we’d like to see a vaccine developed against this bug before it mutates resistance to all available antibiotics. The folks at the MIPnews desk would hate to have to try to convince Dr. Schweizer not to name this possible novel super multidrug-resistant but inherently unstable killer bacteria “B. psupercalifragalisticexpimelioidosis”….
MIP Publications October 2013
Akkina R. New insights into HIV impact on hematopoiesis. Blood. 2013 Sep 26;122(13):2144-6. doi: 10.1182/blood-2013-08-518274.
McGrew AK, Ballweber LR, Moses SK, Stricker CA, Beckmen KB, Salman MD, O'Hara TM. Mercury in gray wolves (Canis lupus) in Alaska: Increased exposure through consumption of marine prey. Sci Total Environ. 2013 Sep 19;468-469C:609-613. doi: 10.1016/j.scitotenv.2013.08.045.
Bohn AA. Diagnosis of disorders of iron metabolism in dogs and cats. Vet Clin North Am Small Anim Pract. 2013 Nov;43(6):1319-30. doi: 10.1016/j.cvsm.2013.07.002.
Ishizaki Y, Hayashi C, Inoue K, Igarashi M, Takahashi Y, Pujari V, Crick DC, Brennan PJ, Nomoto A. Inhibition of the First Step in Synthesis of the Mycobacterial Cell Wall Core, Catalyzed by the GlcNAc-1-phosphate Transferase WecA, by the Novel Caprazamycin Derivative CPZEN-45. J Biol Chem. 2013 Oct 18;288(42):30309-19. doi: 10.1074/jbc.M113.492173.
Briese T, Calisher CH, Higgs S. Viruses of the family Bunyaviridae: Are all available isolates reassortants? Virology. 2013 Nov;446(1-2):207-16. doi: 10.1016/j.virol.2013.07.030.
Safi H, Lingaraju S, Amin A, Kim S, Jones M, Holmes M, McNeil M, Peterson SN, Chatterjee D, Fleischmann R, Alland D. Evolution of high-level ethambutol-resistant tuberculosis through interacting mutations in decaprenylphosphoryl-β-D-arabinose biosynthetic and utilization pathway genes. Nat Genet. 2013 Oct;45(10):1190-7. doi: 10.1038/ng.2743.
Tarigo JL, Scholl EH, McK Bird D, Brown CC, Cohn LA, Dean GA, Levy MG, Doolan DL, Trieu A, Nordone SK, Felgner PL, Vigil A, Birkenheuer AJ. A novel candidate vaccine for cytauxzoonosis inferred from comparative apicomplexan genomics. PLoS One. 2013 Aug 20;8(8):e71233. doi: 10.1371/journal.pone.0071233.
Nandakumar S, Kannanganat S, Dobos KM, Lucas M, Spencer JS, Fang S, McDonald MA, Pohl J, Birkness K, Chamcha V, Ramirez MV, Plikaytis BB, Posey JE, Amara RR, Sable SB. O-mannosylation of the Mycobacterium tuberculosis Adhesin Apa Is Crucial for T Cell Antigenicity during Infection but Is Expendable for Protection. PLoS Pathog. 2013 Oct;9(10):e1003705. doi: 10.1371/journal.ppat.1003705.
Madl JE, Duncan CG, Stanhill JE, Tai PY, Spraker TR, Gulland FM. Oxidative Stress and Redistribution of Glutamine Synthetase in California Sea Lions (Zalophus californianus) with Domoic Acid Toxicosis. J Comp Pathol. 2013 Sep 21. doi:pii: S0021-9975(13)00134-5. 10.1016/j.jcpa.2013.07.012.
Silva EB, Goodyear A, Sutherland MD, Podnecky NL, Gonzalez-Juarrero M, Schweizer HP, Dow SW. Correlates of Immune Protection Following Cutaneous Immunization with an Attenuated Burkholderia pseudomallei Vaccine. Infect Immun. 2013 Oct 7. [Epub ahead of print]
Souza C, Davis WC, Eckstein TM, Sreevatsan S, Weiss DJ. Mannosylated lipoarabinomannans from mycobacterium avium subsp. Paratuberculosis alters the inflammatory response by bovine macrophages and suppresses killing of mycobacterium avium subsp. Avium organisms. PLoS One. 2013 Sep 30;8(9):e75924. doi: 10.1371/journal.pone.0075924.
Weller CB, Cadmus KJ, Ehrhart EJ, Powers BE, Pabilonia KL. Detection and isolation of Influenza A virus subtype H1N1 from a small backyard swine herd in Colorado. J Vet Diagn Invest. 2013 Sep 30.
Goncarovs-Gran KO, Frank CB, Baird AN, Couetil LL, Ramos-Vara JA. Pathology in practice. J Am Vet Med Assoc. 2013 Oct 15;243(8):1113-5. doi: 10.2460/javma.243.8.1113.
Henderson DM, Manca M, Haley NJ, Denkers ND, Nalls AV, Mathiason CK, Caughey B, Hoover EA. Rapid Antemortem Detection of CWD Prions in Deer Saliva. PLoS One. 2013 Sep 11;8(9):e74377. doi: 10.1371/journal.pone.0074377.
Davidson RM, Hasan NA, de Moura VC, Duarte RS, Jackson M, Strong M. Phylogenomics of Brazilian epidemic isolates of Mycobacterium abscessus subsp. bolletii reveals relationships of global outbreak strains. Infect Genet Evol. 2013 Sep 18;20C:292-297. doi: 10.1016/j.meegid.2013.09.012.
Mistry A, Warren MS, Cusick JK, Karkhoff-Schweizer RR, Lomovskaya O, Schweizer HP. High-Level Pacidamycin Resistance in Pseudomonas aeruginosa Is Mediated by an Opp Oligopeptide Permease Encoded by the opp-fabI Operon. Antimicrob Agents Chemother. 2013 Nov;57(11):5565-71. doi: 10.1128/AAC.01198-13.
Shanley CA, Streicher EM, Warren RM, Victor TC, Orme IM. Characterization of W-Beijing isolates of Mycobacterium tuberculosis from the Western Cape. Vaccine. 2013 Oct 18. doi:pii: S0264-410X(13)01420-5. 10.1016/j.vaccine.2013.10.044.
Pabilonia KL, Podell BK, Powers BE. Mast cell tumor (MCT) of the oral mucosa with submandibular lymph node metastasis in a dog. J Am Vet Med Assoc. 2013 Sep 15;243(6):795-7. doi: 10.2460/javma.243.6.795.
Goodyear A, Strange L, Rholl DA, Sirisouk J, Dance DA, Schweizer HP, Dow S. An Improved Selective Culture Medium Enhances the Isolation of Burkholderia pseudomallei from Contaminated Specimens. Am J Trop Med Hyg. 2013 Sep 23.
Loria-Cervera EN, Sosa-Bibiano EI, Villanueva-Lizama LE, Van Wynsberghe NR, Schountz T, Andrade-Narvaez FJ. Cloning and sequence analysis of Peromyscus yucatanicus (Rodentia) Th1 (IL-12p35, IFN-γ and TNF) and Th2 (IL-4, IL-10 and TGF-β) cytokines. Cytokine. 2013 Oct 10. doi:pii: S1043-4666(13)00684-4. 10.1016/j.cyto.2013.09.006.
Michel B, Ferguson A, Johnson T, Bender H, Meyerett-Reid C, Wyckoff AC, Pulford B, Telling GC, Zabel MD. Complement protein C3 exacerbates prion disease in a mouse model of chronic wasting disease. Int Immunol. 2013 Sep 13. [Epub ahead of print]
Piccardo P, King D, Telling G, Manson JC, Barron RM. Dissociation of prion protein amyloid seeding from transmission of a spongiform encephalopathy. J Virol. 2013 Nov;87(22):12349-56. doi: 10.1128/JVI.00673-13.
Haldiman T, Kim C, Cohen Y, Chen W, Blevins J, Qing L, Cohen ML, Langeveld J, Telling GC, Kong Q, Safar JG. Co-existence of Distinct Prion Types Enables Conformational Evolution of Human PrPSc by Competitive Selection. J Biol Chem. 2013 Oct 11;288(41):29846-61. doi: 10.1074/jbc.M113.500108.
Mark Your Calendars!
The second annual Colorado State University reception for pathology alumni and friends will be held at the 2013 ACVP and ASVCP Annual Meeting.
Monday, Nov 18, 7-9 p.m.
Pictured above are the attendees of the Fall meeting of the RM-ASM Branch that took place on Oct 25 & 26 in the Hensel-Phelps West Auditorium at the UC Denver Anschutz Medical Campus.
8th Annual Exhibition
This exhibition showcases the creative energies of students, faculty, and staff at Colorado State University and exemplifies the common ties between scientific inquiry and artistic practice.
Countdown to ASV2014
Next June, we’ll be hosting the large (1,200+ participant) 33rd Annual meeting of the American Society for Virology right here on the CSU campus! Please mark your calendars for this special, can’t-be-missed event. Also – we’ll be looking for numerous (50+) volunteers to help with numerous AV/customer service aspects of this event – so please consider helping us out when asked.
This is a golden opportunity to showcase MIP’s infectious disease expertise and high-quality environment to a large international group of experts in the field. We’ll definitely need the collective help of the MIP community to make this a truly special meeting. For further information on the event, please contact Jeff Wilusz (local lead organizer) at email@example.com.
Rocky Mountain Virology Club Annual Meeting
The 13th Annual Rocky Mountain Virology Club Meeting was held September 27-29 in beautiful Pingree Park, Colorado.
See some Photos from the event!
Immunologist and MIP Alum Leo LeFrancois passed away this summer. Leo graduated from CSU's Microbiology program in 1978. He was very well known for his ideas about how memory develops in the immune system.
Microbiology Tutors for Fall 2013
Students: Please contact tutors directly to make an appt.
Ongoing/Upcoming Construction Project Notes
Micro A and B-Wing Roof Replacement Project – This project suffered numerous delays due to inclement weather but to everyone's relief the work was finally finished on Oct 25th.
Micro B-Wing Freezer Room Project – The work on the 4th floor is largely completed and equipment has been moved in. Work on the 3rd floor began this week and is estimated to take 6 weeks, finishing around the end of November and then the crew will move down to the 2nd floor with a goal of finishing the project around the first of the new year.
Micro A103 Grad Student Break Room Remodel – Work on the break room finished. Once the room is furnished it will be ready for use.
Project meetings for the Roof and Freezer Projects are held every Tuesday morning at 8:30am in Micro B124. Anyone who has questions about either project are encouraged to attend.
K99 Good Morning Guys Win CMA Award
Local radio station K99's Good Morning Guys, Brian Gary, Susan Moore, and Todd Harding (Jenny Harding's husband), have been named small market personalities of the year by the Country Music Association.
Dr. Hoover recently had both knees replaced. The lab formally welcomed him back from his remarkably short absense, and as you can see several lab mates bit the bullet and had knee surgery in solidarity…
New Grant Awards
Anne Avery, "Discovery of Novel Protein, Blood and Epigenetic Biomarkers of Lymphoma Risk, Classification and Prognosis in Golden...", University of Missouri
Candace Mathiason, "Sterilization and Disposal of Agricultural Quarantine Waste", USDA-APHIS
Ian Orme, "Biology of High Transmission Tuberculosis ", Bill and Melinda Gates Foundation
Ian Orme, "Recombinant Yeast-based T-cell Vaccines for TB Infection", GlobeImmune, Inc.
Herbert Schweizer, "Antimicrobial Susceptibility Testing of BARDA B. mallei Strain Panel", Battelle Memorial Institute
Darci Smith, "Characterization of Lead FDA Approved Drugs Effective Against Alphaviruses", DOD-ARMY
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