Vol. 10 | No. 1
Congratulations to Dr. Barry Beaty for receiving the Richard Moreland Taylor Award from the American Committee on Arthropod-Borne Viruses at the American Society of Tropical Medicine and Hygiene meeting in Atlanta in December. Dr. Beaty shared the award with C.J. Peters (pictured). The Richard M. Taylor Award is given every three years to a person who has made outstanding contributions to arbovirology throughout his or her career.
Congratulations to Crystal Shanley of the Orme Laboratory for receiving the Vice President for Research Bright Spot Award! Crystal was nominated for the award for her innovative approaches and organizational/leadership skills that have influenced the success of the Orme Laboratory. Specifically noted was the key role she played in the development of new protocols for feeding anti-TB drugs to infected guinea pigs. These protocols have been adopted by other laboratories in the TB field.
The Bright Spot Award was created to recognize employees who are exceptional in the work they do. By doing things differently, they contribute to higher productivity, cost-efficiency, service and excellence.
Crystal will receive her award at the January Bright Spot Recognition Luncheon on January 31, and a Today@Colostate feature story will be shared with the CSU community in February.
Congratulations to Shannon McLeland for receiving a Morris Animal Foundation Fellowship. Shannon is a post-DVM Fellow investigating the immunopathology of feline chronic kidney disease in the Dow Laboratory.
Congratulations to Hannah Romo, Postdoc in the Quackenbush Lab, for being awarded an NIH F31 award!
Congratulations to Ashley Quick Bear of the Basaraba Laboratory for receiving funding for her proposal entitled, "Immunofluoresecent detection of glucose and fatty acid transporters in diabetic guinea pigs with tuberculosis" as part of the Minority Undergraduate Internship Award through the American Diabetes Association's Research Program.
In the News...
Mercedes Gonzalez-Juarrero and Anne Lenaerts received a shout out for their research in an article entitled ‘Pitt Enzyme Discovery May Lead To Better Tests For Tuberculosis’ that appeared in Medical News Today on January 9th.
Anne Lenaerts and Ian Orme were highlighted in an article entitled ‘New TB drug tested by CSU professors’ that appeared in the Coloradoan on Jan. 5th.
Brian Foy’s work on insecticides was highlighted in an article entitled ‘A new tool against bedbugs: A pill’ that appeared on Jan. 1st in the online Singapore news journal Today.
In Vivo Blockade of the PD-1 Receptor Suppresses HIV-1 Viral Loads and Improves CD4+ T Cell Levels in Humanized Mice
Brent Palmer, Preston Neff, Jonathan LeCureux, Angelica Ehler, Michelle DSouza, Leila Remling-Mulder, Alan Korman, Andrew Fontenot and Ramesh Akkina
J. Immunology 190: 211-219 (Jan. 2013)
In a Disney-esque way, Ramesh Akkina’s lab specializes in giving human traits to animals. However there’s nothing Mickey Mouse about the work they do. Ramesh and colleagues favorite thing to do is to inject hematopoietic stem cells into mice that lack an immune system of their own. This gives the little critters a human-type immune system, allowing them to be used to study aspects of how the human immune system functions in vivo. Lately, the Akkina lab has been using these model animals to assess a variety of therapies to combat HIV infection. The therapy that they test in this study in collaboration with folks from the Anschutz Medical Campus and Bristol Meyers Squibb is particularly exciting.
I bet that many of you know the basics of what happens when you are infected with HIV. The virus attacks and kills a subset of T cells called CD4+, resulting in high viral loads and a progressive weakening of the ability of the patient’s immune system to combat infections. ‘Nuff said – right? Well a detail that’s not highlighted in most textbooks is that during chronic viral infection and cancer, the surviving stressed T cells upregulate a ‘death receptor’ called PD-1. This makes the T cells function less efficiently and are more likely to undergo programmed cell death. Clinical trials are underway using monoclonal antibodies to block this PD-1 death receptor as a way to treat cancers such as melanoma. Could such a treatment also be effective as a way to help treat HIV infections? Great question – all we need is a way to test it. However it is not an easy task given that HIV only infects humans. That bugling sound you hear in the distance is Ramesh Akkina and his cavalry of humanized mice coming to the rescue.
In this study they tested the efficacy of a PD-1 monoclonal antibody (mAb) provided by Bristol-Meyers-Squibb in treating HIV-infected humanized mice. The results that were obtained were extremely encouraging. Mice that received the PD-1 mAb had up to a 3 log lower (that’s 1000X!) HIV titers in their blood, solid 3-5X increases in the number of CD4+ T cells and clear evidence for increased Th1 cytokines (that are associated with effective immune responses against viral infections). I bet even the normally stoic Dr. Akkina had a big smile on his face when he first gazed upon these positive data.
So why did we pick this paper for our coveted MIPublication of the Month® rather than the exciting study by Candace Mathiason et al on the transmission of CWD prions to cats or the nice work by Meltesen and Bohn on refining ways to assess acid base balances in dogs? Three reasons, my patient reader. First, while drugs targeting virus-specific functions are well-developed for HIV treatment, the generation of drugs therapies that directly augment an HIV-infected patient’s immune system is in its infancy. Thus this study may give this interesting PD-1 receptor targeting approach a real shot in the arm. Second, the paper is a great example of the type of translational research that many MIP labs are pursuing in addition to the classic basic science that are the foundation of our academic research. Finally, we hope you notice that we’re taking great efforts to try to ‘humanize’ the MIPnews this year – and figure highlighting this paper is a great way to start.
MIP Publications January 2013
Readers: We know that you are all eager to read about exciting MIP scientific discoveries ASAP. Thus please note that beginning this month we will report publications as soon as they are listed on PubMed rather than wait for the full citation to arrive as we have done in the past.
Phillips AT, Stauft CB, Aboellail TA, Toth AM, Jarvis DL, Powers AM, Olson KE. Bioluminescent Imaging and Histopathologic Characterization of WEEV Neuroinvasion in Outbred CD-1 Mice. PLoS One. 2013;8(1):e53462.
Zhou J, Neff CP, Swiderski P, Li H, Smith DD, Aboellail T, Remling-Mulder L, Akkina R, Rossi JJ. Functional In Vivo Delivery of Multiplexed Anti-HIV-1 siRNAs via a Chemically Synthesized Aptamer With a Sticky Bridge. Mol Ther. 2013 Jan;21(1):192-200.
Aboellail TA. Pathologic and immunophenotypic characterization of 26 camelid malignant round cell tumors. J Vet Diagn Invest. 2013 Jan 4. [Epub ahead of print]
Muramoto Y, Noda T, Kawakami E, Akkina R, Kawaoka Y. Identification of novel influenza A virus proteins translated from PA mRNA. J Virol. 2012 Dec 12. [Epub ahead of print]
Akkina R. New generation humanized mice for virus research: Comparative aspects and future prospects. Virology. 2013 Jan 5;435(1):14-28.
Palmer BE, Neff CP, Lecureux J, Ehler A, Dsouza M, Remling-Mulder L, Korman AJ, Fontenot AP, Akkina R. In Vivo Blockade of the PD-1 Receptor Suppresses HIV-1 Viral Loads and Improves CD4+ T Cell Levels in Humanized Mice. J Immunol. 2013 Jan 1;190(1):211-9.
Speer A, Shrestha TB, Bossmann SH, Basaraba RJ, Harber GJ, Michalek SM, Niederweis M, Kutsch O, Wolschendorf F. Copper-Boosting Compounds: A Novel Concept for Anti-Mycobacterial Drug Discovery. Antimicrob Agents Chemother. 2012 Dec 17. [Epub ahead of print]
Irie Y, Borlee BR, O'Connor JR, Hill PJ, Harwood CS, Wozniak DJ, Parsek MR. Self-produced exopolysaccharide is a signal that stimulates biofilm formation in Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 2012 Dec 11;109(50):20632-6.
Voge NV, Sánchez-Vargas I, Blair CD, Eisen L, Beaty BJ. Detection of Dengue Virus NS1 Antigen in Infected Aedes aegypti Using a Commercially Available Kit. Am J Trop Med Hyg. 2012 Nov 26. [Epub ahead of print]
Graham CB, Black WC, Boegler KA, Montenieri JA, Holmes JL, Gage KL, Eisen RJ. Combining real-time polymerase chain reaction using SYBR Green I detection and sequencing to identify vertebrate bloodmeals in fleas. J Med Entomol. 2012 Nov;49(6):1442-52.
Van Zee J, Black WC 4th, Levin M, Goddard J, Smith J, Piesman J. High SNP density in the blacklegged tick, Ixodes scapularis, the principal vector of Lyme disease spirochetes. Ticks Tick Borne Dis. 2012 Dec 6. doi:pii: S1877-959X(12)00075-1. 10.1016/j.ttbdis.2012.07.005. [Epub ahead of print]
Graham CB, Borchert JN, Black WC 4th, Atiku LA, Mpanga JT, Boegler KA, Moore SM, Gage KL, Eisen RJ. Blood Meal Identification in Off-Host Cat Fleas (Ctenocephalides felis) from a Plague-Endemic Region of Uganda. Am J Trop Med Hyg. 2012 Dec 3. [Epub ahead of print]
Meltesen HS, Bohn AA. Using corrected serum chloride and predicted bicarbonate concentrations to interpret acid-base status in dogs. Vet Clin Pathol. 2012 Dec;41(4):509-17.
Kim HJ, Prithiviraj K, Groathouse N, Brennan PJ, Spencer JS. Gene expression profile and immunological evaluation of unique hypothetical unknown proteins of Mycobacterium leprae by using quantitative real time-PCR. Clin Vaccine Immunol. 2012 Dec 12. [Epub ahead of print]
Reagan KL, Machain-Williams C, Wang T, Blair CD. Immunization of Mice with Recombinant Mosquito Salivary Protein D7 Enhances Mortality from Subsequent West Nile Virus Infection via Mosquito Bite. PLoS Negl Trop Dis. 2012 Dec;6(12):e1935.
Van Regenmortel MH, Ackermann HW, Calisher CH, Dietzgen RG, Horzinek MC, Keil GM, Mahy BW, Martelli GP, Murphy FA, Pringle C, Rima BK, Skern T, Vetten HJ, Weaver SC. Virus species polemics: 14 senior virologists oppose a proposed change to the ICTV definition of virus species. Arch Virol. 2012 Dec 27. [Epub ahead of print]
Blitvich BJ, Saiyasombat R, Travassos da Rosa A, Tesh RB, Calisher CH, Garcia-Rejon JE, Farfán-Ale JA, Loroño RE, Bates A, Loroño-Pino MA. Orthobunyaviruses, a Common Cause of Infection of Livestock in the Yucatan Peninsula of Mexico. Am J Trop Med Hyg. 2012 Dec;87(6):1132-1139.
Mahapatra S, Piechota C, Gil F, Ma Y, Huang H, Scherman MS, Jones V, Pavelka MS Jr, Moniz-Pereira J, Pimentel M, McNeil MR, Crick DC. Mycobacteriophage Ms6 LysA is a peptidoglycan amidase and a useful analytical tool. Appl Environ Microbiol. 2012 Nov 16. [Epub ahead of print]
Takaesu G, Inagaki M, Takubo K, Mishina Y, Hess PR, Dean GA, Yoshimura A, Matsumoto K, Suda T, Ninomiya-Tsuji J. TAK1 (MAP3K7) Signaling Regulates Hematopoietic Stem Cells through TNF-Dependent and -Independent Mechanisms. PLoS One. 2012;7(11):e51073.
Mitchell LA, Hansen RJ, Beaupre AJ, Gustafson DL, Dow SW. Optimized dosing of a CCR2 antagonist for amplification of vaccine immunity. Int Immunopharmacol. 2012 Dec 12. doi:pii: S1567-5769(12)00359-1. 10.1016/j.intimp.2012.11.016. [Epub ahead of print]
Guth AM, Hafeman SD, Dow SW. Depletion of phagocytic myeloid cells triggers spontaneous T cell- and NK cell-dependent antitumor activity. Oncoimmunology. 2012 Nov 1;1(8):1248-1257.Mitchell LA, Henderson AJ, Dow SW. Suppression of vaccine immunity by inflammatory monocytes. J Immunol. 2012 Dec 15;189(12):5612-21.
Schaffer PA, Wobeser B, Martin LE, Dennis MM, Duncan CG. Cutaneous neoplastic lesions of equids in the central United States and Canada: 3,351 biopsy specimens from 3,272 equids (2000-2010). J Am Vet Med Assoc. 2013 Jan 1;242(1):99-104.
Malboeuf CM, Yang X, Charlebois P, Qu J, Berlin AM, Casali M, Pesko KN, Boutwell CL, Devincenzo JP, Ebel GD, Allen TM, Zody MC, Henn MR, Levin JZ. Complete viral RNA genome sequencing of ultra-low copy samples by sequence-independent amplification. Nucleic Acids Res. 2013 Jan 1;41(1):e13.
Myers E, Ehrhart EJ, Charles B, Spraker T, Gelatt T, Duncan C. Apoptosis in Normal and Coxiella burnetii-Infected Placentas From Alaskan Northern Fur Seals (Callorhinus ursinus). Vet Pathol. 2012 Nov 2. [Epub ahead of print
Yang Y, Bhatti A, Ke D, Gonzalez-Juarrero M, Lenaerts A, Kremer L, Guerardel Y, Zhang P, Ojha AK. Exposure to a Cutinase-like Serine Esterase Triggers Rapid Lysis of Multiple Mycobacterial Species. J Biol Chem. 2013 Jan 4;288(1):382-92.
Mathiason CK, Nalls AV, Seelig DM, Kraft SL, Carnes K, Anderson KR, Hayes-Klug J, Hoover EA. Susceptibility of domestic cats to chronic wasting disease. J Virol. 2012 Dec 12. [Epub ahead of print]
Denkers ND, Hayes-Klug J, Anderson KR, Seelig DM, Haley NJ, Dahmes SJ, Osborn DA, Miller KV, Warren RJ, Mathiason CK, Hoover EA. Aerosol Transmission of Chronic Wasting Disease in White-tailed Deer. J Virol. 2012 Nov 21. [Epub ahead of print]
Perrott MR, Sigurdson CJ, Mason GL, Hoover EA. Mucosal transmission and pathogenesis of chronic wasting disease in ferrets. J Gen Virol. 2012 Oct 24. [Epub ahead of print]
Grzegorzewicz AE, Jackson M. Subfractionation and Analysis of the Cell Envelope (Lipo)polysaccharides of Mycobacterium tuberculosis. Methods Mol Biol. 2013;966:309-24.
Olver CS, Nielsen VG. Thrombelastographic characterization of coagulation/fibrinolysis in horses: role of carboxyheme and metheme states. Blood Coagul Fibrinolysis. 2012 Dec 4. [Epub ahead of print]
Ryseff JK, Olver CS. What is your diagnosis? Ventral neck mass in a cat. Vet Clin Pathol. 2012 Dec;41(4):603-4.
Magden E, Miller C, Macmillan M, Bielefeldt-Ohmann H, Avery A, Quackenbush SL, Vandewoude S. Acute virulent infection with feline immunodeficiency virus (FIV) results in lymphomagenesis via an indirect mechanism. Virology. 2013 Jan 2. doi:pii: S0042-6822(12)00595-8. 10.1016/j.virol.2012.12.003. [Epub ahead of print]
Kingry LC, Cummings JE, Brookman KW, Bommineni GR, Tonge PJ, Slayden RA. The Francisella tularensis FabI Enoyl-Acyl Carrier Protein Reductase Gene Is Essential to Bacterial Viability and Is Expressed during Infection. J Bacteriol. 2013 Jan;195(2):351-8.
Schweizer HP. Mechanisms of antibiotic resistance in Burkholderia pseudomallei: implications for treatment of melioidosis. Future Microbiol. 2012 Dec;7:1389-99.
Kvitko BH, Cox CR, Deshazer D, Johnson SL, Voorhees KJ, Schweizer HP. phiX216, a P2-like bacteriophage with broad Burkholderia pseudomallei and B. mallei strain infectivity. BMC Microbiol. 2012 Dec 7;12(1):289.
Damron FH, McKenney ES, Schweizer HP, Goldberg JB. Construction of a Broad-Host-Range Tn7-Based Vector for Single-Copy PBAD-Controlled Gene Expression in Gram-Negative Bacteria. Appl Environ Microbiol. 2013 Jan;79(2):718-21.
Spencer, JS, Duthie MS, Geluk A, Balagon MF, Kim HJ, Wheat WH, Chatterjee D, Jackson M, Li W, Kurihara JN, Maghanoy A, Mallari I, Saunderson P, Brennan PJ, Dockrell HM. Identification of serological biomarkers of infection, disease progression and treatment efficacy in leprosy. Mem Inst Oswaldo Cruz, Rio de Janeiro, Vol 107 (Suppl.1): 79-82 2012.
Godwin AR, Kojima S, Green CB, Wilusz J. Kiss your tail goodbye: The role of PARN, Nocturnin, and Angel deadenylases in mRNA biology. Biochim Biophys Acta. 2012 Dec 26. doi:pii: S1874-9399(12)00210-6. 10.1016/j.bbagrm.2012.12.004. [Epub ahead of print]
To Or Friends at the Department of Microbiology, Immunology & Pathology~
We would like to thank you for your generous contribution to our Christmas Angel Program. We cannot thank you enough for your interest in choosing our organization. We hope you know that your support and dedication are vital to the success of our program.
Every holiday season, families face financial,emotional and unfortunate crises. Due to unemployment, disabilities, or sudden tragedies, they find it necessary to ask The Salvation Army for support. Many of these families have little or no hope during this season and it is your generosity and commitment to our clients that makes all the difference. We witness first-hand the tears that your generosity brings to our clients' eyes. We also know that, for many of our clients, your gifts go beyond providing basic needs; they instill back the hope that has been lost to hardship.
Together, we were able to help 995 families this Christmas season: 1995 children received 8775 toys, 149 senior residents were visited at the nursing homes and enjoyed company and gifts, and 800 families had holiday food on their tables.
Once again we thank you for your dedication and partnership in doing the most good, to those who need it the most. We look forward to serving even more families next year with your help.
May God Bless!
Robert Covert, Captain
SAVE THE DATE!
The Mycobacteria Research Laboratories will be hosting the very first Front Range Mycobacteria Conference this June at the University Center for the Arts. Stay tuned for more information and check out the newly-redesigned MRL website for a link to online registration.
CONGRATULATIONS to the Dlab for attaining AAVLD accreditation through November 2017!
Check out this and what else is happening at the Diagnostic Laboratory in the Winter 2012 Lablines
Attention all Micro Majors
MSA meetings will held Tuesdays at 5:30pm this semester. Our first meeting (1/29) is a can’t-be-missed welcome back movie night featuring the provocative sci-fi drama "GATTACA". Food, drinks and molecular biology puns will provided for attendees. On 2/5 we will be holding a Study Help Night. MSA officers will be on hand to answer questions/tutor in any of the MIP prerequisites/MIP classes. Hope to see y’all there.
Deadline: March 25 at 5PM
CMB/MCIN/BMB/MIP Poster Symposium
It is time again for food, fun, drink and a little competition. Please think ahead and consider presenting a poster for the Annual Cell & Molecular Biology/Molecular, Cellular & Integrative Neurosciences/Biochemistry & Molecular Biology & Microbiology, Immunology & Pathology poster symposium. Presenters are welcome to use posters presented at other symposiums. This is also graduate recruitment weekend for these programs.
See the abstract submission flyer for more information. Submission Deadline is February 8th!
The 14th Annual CVMBS Research Day will be held on January 26, 2013 at the Hilton, Fort Collins.
The American Society for Microbiology is currently accepting applications for their ASM Undergraduate Research Fellowship
The deadline is February 1st!
The American Heart Association and the American Stroke Association undergraduate student research program is designed to inspire promising students from all disciplines, including women and members of minority groups underrepresented in the sciences, to consider research careers while supporting the highest quality scientific investigation broadly related to cardiovascular disease and stroke. The research opportunity will allow studentst to work for 10 weeks with a faculty/staff member on any project broadly related to cardiovascular function and diseases, stroke, or to associated basic science, clinical, bioengineering/biotechnology and public health problems.
To Apply Go To www.heart.org!
Look for Healthcare/Research Tab, Click on Research, then on Funding Opportunities.
The deadline is February 7th!
Best wishes to Vara Vissa and Alexander Franz, special appointment faculty who recently left the department. You will be missed!
Hi everyone, my name is Jennifer Kahrs and I am the new Facilities Coordinator for MIP. I grew up on a dryland wheat farm near Otis Colorado. I came to CSU to get my Bachelor’s degree in Biological Sciences and never left! Some of you might know me already from my prior position doing capital equipment inventories for Business and Financial Services. I did that for 8 years and while I enjoyed it a lot, it was time for a change! I am very excited to be a part of the MIP department and I look forward to meeting all of you!
Looking for a way to de-stress at the end of a long day?
What: Free yoga!
The class will be taught by Carol Borchert, a certified yoga instructor with 10 years of experience. She’ll be teaching a variety of styles, from flow yoga to hatha to restorative. This class is open to everyone – whether you have never done yoga before or are an experienced practitioner. Please bring a yoga mat and wear comfortable workout clothing. Sponsored by the Department of Microbiology, Immunology and Pathology
Enjoy an evening with the Foothills Pops and Swing Band on February 9 while enjoying cake, coffee, light hors d'oeuvres and a cash bar at the Embassy Suites (next to the Ranch). Doors open at 7pm, entertainment by 45-piece pops band from 7:30-8:20 with the 18-piece big band style jazz band playing from 8:30-11pm for big band tunes and dancing. MIP's very own Glenda Allen plays the alto saxophone in the band. See the attached flyer for more information.
Congratulations to our very own Jeff Wilusz (the short dude in the back) for receiving a 2012 Coaching Excellence Award from the Fort Collins Soccer Club.
In early January, while Fort Collins was experiencing sub-freezing temperatures, John Spencer was vacationing in Hawaii...
"It has been raining off and on for 8 days, but every day in Hawaii is still paradise. Have been running every morning 5-10K, temps in the high 70s, so perfect running weather. Today was the first sunny day, so took my daughter boogey boarding at Kailua beach, caught some nice waves. Below are pictures of my favorite beach at Makapuu..."
The Mathiason-Hoover-VandeWoude-Zabel labs participated in a 2012 Christmas Tree Ornament Contest. Three ornament makers won ‘Fabulous Prizes'... First place: Erin McNulty and Stephenie Fullaway, Second place: Amber Mayfield, Third place: Amy Nalls
The Karen Dobos Lab took part in a service project on December 19th during their holiday party. In addition to fun and food, they made ornaments for the patients at Children's Hospital in Denver. They made about 250 reindeer, snowmen, and snowflake ornaments filling the afternoon with glitter, glue, and laughter for a good cause. The ornaments were delivered to hospital volunteer staff who then distributed them out to the children on Christmas Eve. Everyone form the lab was so happy to take part of a great cause and it's something we'll most likely take part in again.
Keri Wright's baby niece Charlotte was born in late August with a rare condition called Cri Du Chat Syndrome, which is the result of a missing piece of chromosome number five. She spent the first 3 months of her life at Children's and ended back there again in January as a patient for severe bacterial infection in her lungs. Therefore, Children's Hospital is near and dear to Keri's heart as she's seen personally how much the children need to be loved and thought of, especially during the holidays.