HomeGlobal LeprosyAvailable MaterialsRequest Research MaterialsSkin TestingBasic ResearchContacts and PersonnelReferences and Links
Leprosy  Research Home CSU Home


Available Materials

IX. Primers

Reagent:

Primer Set

Quantities available:

10 µM when reconstituted in 200 µL volume

Products Available:

 

Table of available primers

Loci Name

Product Size (bp)

(C)20

197

(TA)18

280

(AT)17

181

(AC)9

236

(GTA)9
307
(AGA)20
201
6-7
270
12-5
289
21-3
312
27-5
327

Production System:

Primers are selected and designed using Oligo 6 Primer Analysis software (Molecular Biology Insights, Inc., Cascade Colo.). All primer sets are then determined to be M. leprae-specific using Blast analysis software from the Leproma Database Website (http://genolist.pasteur.fr/Leproma/). Synthesis of these oligonucleotides is performed at the Macromolecular Resource Facility; a core facility here at Colorado State University. All oligonucleotides are synthesized and RP-1 purified in house to ensure that they are essentially free of truncated oligonucleotides, organic salts, and residual synthesis chemistry. Each oligonucleotide is lyophilized and stored at -20°C. Reconstitution in 200 μL of ddH 2O will result in 10 μM stock solution.

Notes:

Quality control is determined by MALDI/TOF mass spectrometry or analytical gel electrophoresis and U.V. spectrophotometry.

References:

Groathouse, N. A., et al., J. Clin. Micro. 42:4, 2004. Cole, S. T., et al., Nature. 409:1007-11, 2001. Shin, Y. C., et al., J. Clin. Microbiol. 38:4535-8, 2000. Int. J. Lepr. Other Mycobact.Dis. 68(2):121-8, 2000.
Back

footer

Contact / Disclaimer / Equal Opportunity / Apply to CSU / Privacy Statement
Copyright © 2007 Colorado State University
This page was last updated October 10, 2007