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Primers used in the PCR amplification of M. leprae STR DNA for strain typing

 
STR Locus Primer Sequence (Foward) T °C Primer Sequence (Reverse) T °C Product Length (bp)a
(C)20 5’TCGGCGACTGCGGTAACATT3’ 62 5’CGAGTGAGCGGAACGGAGTA3’ 60 197
(TA)18 5’CGTGCGTCGTGTGTAGGCA3’ 62 5’GACGTGGCAACATCGAAGTT3’ 60 230
(AT)17 5’TCTCCAACATGCTGCGACA3’ 60 5’GTACAGCGGCCTGATCGAA3’ 60 181
(AC)9 5’AGCGCCCGTTGTCGATAGA3’ 60 5’TGCTGGTGACCCGCGATA3’ 58 236
(GTA)9 5’AGCCTTAGTCGCGCAGATG3’ 60 5’TCCGCTGTCCGTCCGCTGA3’ 64 307
(AGA)20 5’GGACCTAAACCATCCCGTTTT3’ 62 5’CTACAGGGGGCACTTAGCTC3’ 64 201
6-7 5’GCCATCGTTGTCGGTTCATC3’ 62 5’CGGAGGAGGTGGGTACGGT3’ 64 268
12-5 5’CTGGTCCACTTGCGGTACG3’ 62 5’GGAGAAGGAGGCCGAATACA3’ 62 289
21-3 5’GAATCTGACCTTTCGGAAATG3’ 60 5’CGATGCAGCTTCCTACGG3’ 58 312
27-5 5’ATTGAGCAGATGGCCGGTC3’ 60 5’AGCAGTCGGCACGCCCTT3’ 60 32

aAmplicon lengths are based on the M. leprae TN sequence.

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This page was last updated October 10, 2007