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II. Whole Cells Lysate

Reagent:

M. leprae Whole Cell Lysate (non-irradiated)

Handling Requirements:

Biosafety Level 2 (BSL2) conditions

Quantities available:

250 micrograms (protein)

Production system:

100 mg of purified whole cells is suspended in 2 ml of phosphate buffered saline and probe sonicated using a Sanyo (MSE) Soniprep 150 Ultrasonic Homogenizer for 40 one minute cycles. An acid fast stain of the sonicate is performed to confirm >95% breakage of the cells. Protein content is quantitated by the BCA assay (Pierce) and the sonicate is aliquoted at 250 micrograms per vial, luophilized, and stored at -80oC.

Notes:

This crude preparation contains proteins, lipids and carbohydrates present within the bacterial cell, including cell wall, cytosol, and membrane.

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This page was last updated October 10, 2007