M. leprae infected armadillo tissue

Homogenization

NaOH treatment
(To degrade host armadillo tissue)

Enzyme digestion
(Collagenase, Trypsin, and Chymotrypsin)

PEG/DEX
(Two phase separation of tissue debris from M. leprae)

Multiple wash of M. leprae

Store at -70°C
Lyophilized and/or irradiated aliquots for shipment