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Available Materials

VII. Genomic DNA

Reagent:

M. leprae genomic DNA from three different isolates (NHDP-63, Br 4923, and Thai-53)

Quantities available:

2 or 5 micrograms

Production system:

Cell lipids are extracted from whole M. leprae bacilli by chloroform/methanol (2:1) extraction in an equal volume of Tris-EDTA buffer. Delipidated cells are exposed to SDS and proteinase K treatment. Genomic DNA is isolated from contaminating proteins using organic extraction. To remove polysaccharide contaminants, DNA is precipitated with isopropanol and stored at -70oC as a dry pellet.

Notes:

Quality control for this reagent includes agarose gel electrophoresis, restriction enzyme digestibility, and spectrophotometric analysis.

References:
Belisle, J.T., and M.G. Sonnenberg, Methods Mol. Biol. 101:31, 1998.
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