What to bring to the Flow Lab

• Your filtered cells
• Extra media/buffer
• Tubes to sort into with your media in the tubes (or whatever buffer you want your sorted cells to land in)
• If using the cloning sort option, your 96 well plates with media in each well
• A sample of unstained cells, pos &/or neg controls, and compensation (single color) controls for each stain

IMPORTANT! BIOHAZARD PRECAUTION!

Due to the fact that cell sorting generates aerosols and the cells are generally not submitted in any fixative, the biohazard risks are much greater than in analytic flow cytometry done on a machine without a jet-in-air flow nozzle. It is therefore imperative to discuss any biohazard issues with Leslie before setting up your experiment. At CSU, we do have an aerosol evacuation system which removes micro droplets and particulates greater than 0.12 micron. They are removed under vacuum, and trapped in an Ultra Low Penetration Air (ULPA) filter. Please inform Leslie of the Biological Safety Level (BSL) of your sample.

GFP transfected cell population prior to sorting

GFP positive cells selected, post sort purity check

 

More Information

• MoFlo Homepage
• Flow Cytometry
• Cell Sorting
• Protocols and Sample Preparation
• Controls needed for Each Experiment
• What to Bring?