Instrumentation

MoFlo (Dako Colorado, Inc.) Flow Cytometer and High Speed Cell Sorter

• Solid state iCyt 488nm (blue) laser
• 635nm (red) diode laser
• Coherent Radius 405nm (violet) laser
• Capable of measuring up to 9 fluorescent parameters in addition to forward and side light scatter
• Sort as many as 4 sub- populations of cells at one time, rather than the standard 2 fractions.
• Sort single cells (or any specified number) into 96 well plates using the automated Cyclone attachment. Other sizes of plates and trays can also be used, and microscope slides.

Due to the complexity of this instrument, all analysis and cell sorting experiments need to be scheduled with the trained operator. The purity and yield of the sort can be maximized according to client needs

Contact Information

Anne Avery
Colorado State University
1619 Campus Delivery
Room 231 Pathology
Fort Collins, CO 80523
(907) 491-6138
Anne.Avery@Colostate.Edu

Leslie Armstrong-Lea
Colorado State University
1619 Campus Delivery
Room 101B Pathology
Fort Collins, CO 80523
(970) 491-1347
Leslie.Armstrong-Lea@Colostate.Edu

Personnel

Leslie Armstrong-Lea has been a flow cytometry technician at CSU since 1986 in Dr. Mike Fox's laboratory, and joined the MoFlo facility in Pathology in 2005. Prior to that, she worked in a clinical flow cytometry laboratory at Swedish Hospital in Denver, Colorado.

More Information

• Flow Cytometry
• Cell Sorting
• Protocols and Sample Preparation
• Controls needed for Each Experiment
• What to Bring?

Services Available

Many possibilities exist for flow cytometric analysis or individual cell sorting, including, but not limited to:

• Immunofluorescent measurements (surface and intracellular markers, single and multi-parameter) for antigen and antibody distributions (up to 9 colors with 3 lasers)
• Determination of cell viability using Propidium Iodide, or PI with Sybr 14, and Dead/Live cell gating
• Expression of Green Fluorescence Protein (GFP), or ds-Red
• Detection of apoptosis with TUNEL or Annexin-V
• Multicolor identification or sorting of bacteria, parasites, neurons, sperm, or RBC’s
• Quantitative analysis of the number of molecules of a particular antigen found on the surface of a cell (MESF)
• Cell division studies using antibodies to bromodeoxyuridine (BrdU)
• DNA and cell cycle analysis, DNA Index for aneuploidy
• Mutant isolation
• Plant cell studies
• Virus infected cells
• Sterile sorting into multi-well plates, trays, or onto individual microscope slides with automated cloning device
• One, two, three or four way sterile tube sorting

Charges

The rates for service for CSU affiliated labs:

• Analysis only is $80 an hour, with a ½ hour set up charge ($40 for set up)
• Sorting is $100 an hour, with a 1 hour set up charge ($100 for set up)
• Please be aware that if you cancel your appointment at the last minute, you are still charged for the set up time. Sorting/analysis charges start at the time of your appointment, not when you arrive
• If you need to cancel your appointment, please do so as early as possible. If you are running late for your appointment, please notify Leslie so she can see if she can arrange for a later starting time for people scheduled after you

Please have the following form completed for billing information the first time you come to the MoFlo lab. This form does not have to be filled out each time, only on fiscal year change, or if your fund number changes.

CSU Users Billing Form

We also perform flow cytometric analysis and sorting for laboratories not affiliated with CSU. Please contact Leslie Armstrong-Lea at (970) 491-1347 for rates for outside services.

Non-CSU Users Billing Form