Karen M Dobos Laboratory
Assistant Professor

Phone: 491-6549
Fax: 491-1815
Email: Karen.Dobos@Colostate.Edu
Office: C223 Microbiology Building
Lab: C210 Microbiology Building

Research Interests

The Dobos laboratory is focused on descriptive, comparative studies of Mycobacterium tuberculosis strains that are relevant to the development of 2nd generation vaccines and diagnostic assays. Specifically, the laboratory utilizes mass spectrometry, animal models of tuberculosis, and collaborations with clinical research groups to define macromolecules engaged in host-parasite interactions. This is accomplished through highly collaborative studies within CSU (principally with the laboratories of Drs. Angelo Izzo and Jessica Prenni) and outside of the University.

An important outcome of this activity is the provision of biological reagents through the Tuberculosis Vaccine Testing and Research Materials Contract (HHSN266200400091c). If you are interested in this NIAID contract activity, please visit our website at: www.cvmbs.colostate.edu/mip/tb/index.htm .

Selected Publications

Pub Med for Dobos KM.

Marques, AM, A.G. Neves-Ferreira, E.K. Xavier da Silveira, R.H. Valente, A. Chapeaurouge, J. Perales, R. da Silva Bernardes, K.M. Dobos, J.T. Belisle, J.S. Spencer, P.J. Brennan, and M.C.V. Pessolani. (2008). Deciphering the proteomic profile of Mycobacterium leprae cell envelope. Proteomics. 8(12): 2477-91.

Sieling PA, Hill PJ, Dobos KM, Brookman K, Kuhlman AM, Fabri M, Krutzik SR, Rea TH, Heaslip DG, Belisle JT, Modlin RL. (2008). Conserved mycobacterial lipoglycoproteins activate TLR2 but also require glycosylation for MHC class II-restricted T cell activation. J Immunol. 180: 5833-42.

Sable, S. B., R. Kumar, M. Kalra, I. Verma, G. K. Khuller, K. Dobos, and J. T. Belisle. (2005). Peripheral blood and pleural fluid mononuclear cell responses to low-molecular-mass secretory polypeptides of Mycobacterium tuberculosis in human models of immunity to tuberculosis. Infect Immun. 73: 3547-58.

Kashyap, R. S., K. M. Dobos, J. T. Belisle, H. J. Purohit, N. H. Chandak, G. M. Taori, and H. F. Daginawala. (2005). Demonstration of components of antigen 85 complex in cerebrospinal fluid of tuberculous meningitis patients. Clin Diagn Lab Immunol. 12: 752-8.

Mawuenyega, K. G., C. V. Forst, K. M. Dobos, J. T. Belisle, J. Chen, E. M. Bradbury, A. R. Bradbury, and X. Chen. (2005). Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Mol Biol Cell. 16:396-404.

Raghunathan, P. L., E. A. S. Whitney, K. Asamoa, Y. Stienstra, G. Amofah, D. Ofori-Adjei, K. Dobos, J. Guarner, S. Kihlstrom, S. Pathak, T. H. Taylor Jr., E. Klutse, S. Etuaful, E. Quarshie, W. van der Graaf, T. van der Werf, C. H. King, J. W. Tappero, D. A. Ashford (2005). Risk Factors for Buruli Ulcer Disease (Mycobacterium ulcerans infection): Results from a Case-Control Study in Ghana. Clin. Infect. Dis. 40: 1445-53.

Stienstra, Y., T. van der Werf, W. van der Graaf, E. Secor, S. Kihlstrom, K. Dobos, K. Asamoa, E. Quarshi, S. N. Etauful, K. Y. Erasmus, and C. H. King. (2004). Buruli Ulcer and Schistosomiasis: No Association Found. Amer. J. Trop. Med. Hygeine. 71: 318-21

Marques, M. A., B. J. Espinosa, E. K. Xavier da Silveira, M. C. Pessolani, A. Chapeaurouge, J. Perales, K. M. Dobos, J. T. Belisle, J. S. Spencer, and P. J. Brennan. (2004). Continued proteomic analysis of Mycobacterium leprae subcellular fractions. Proteomics. 4: 2942-53.

Hmama, Z., K. Sendide, A. Talal, R. Garcia, K. Dobos, N.E. Reiner (2004). Quantitative analysis of phagolysosome fusion in intact cells: inhibition by mycobacterial lipoarabinomannan and rescue by an 1{alpha},25-dihydroxyvitamin D3-phosphoinositide 3-kinase pathway. J Cell Sci. 117: 2131-2140.

Gehring, A.J., K.M. Dobos, J.T. Belisle, C.V. Harding, and W.H. Boom. (2004). Mycobacterium tuberculosis LprG (Rv1411c): A novel Toll-like receptor-2 ligand that inhibits human macrophage class-II MHC antigen processing. J. Immunol. 173: 2660-68

Turner, J., K.M. Dobos, M.A. Keen, A.A. Frank, S. Ehlers, I.M. Orme, J.T. Belisle, and A.M. Cooper. (2004). A limited antigen-specific cellular response is sufficient for the early control of M. tuberculosis in the lung but is insufficient for long-term survival. Infect. Immun. 72: 3759-68.