MIP Typhoon Trio Imaging Facility
The Typhoon Trio is available to CSU researchers for imaging of fluorescent and radioactive experiments. It is housed in Microbiology Room B319. This room is open during normal working hours.
Contact: Carol Wilusz: cwilusz@colostate.edu tel:491-4919, office: Micro B407, lab: Micro B429.
Phosphorimaging:
Phosphor storage screens are available for communal use. Please treat them with care – they cost over $1000 each. Following exposure to the screen, the images are visualized by scanning in storage phosphor mode at 200mM resolution. Screens are available for general (32P, 35S) and tritium isotopes.
Fluorescent Imaging:
The Typhoon has three lasers enabling excitation and visualization of a wide range of wavelengths (link to Table for scanning info). Applications include fluorescent stained protein gels (SYPRO Ruby, Deep Purple), fluorescent western blots (ECL Plus, ECL Plex), fluorescent nucleic acid stains (SYBR-green), 2D-DIGE (Cy2, Cy3, Cy5) etc. Gels and blots are positioned directly on a glass platen for scanning.
The Typhoon is not recommended for straight chemiluminescence, but fluorescent alternatives such as ECL Plus, and Cy-dye labeled secondary antibodies do allow western blotting experiments to be analyzed. Please ask as we may have samples available for you to test.
Downstream applications:
Images can be saved as TIFF files for manipulation in standard graphic software. Analysis is performed using ImageQuantTL software which is loaded on the Typhoon computer.
A robotic spot-picker and digester (funded by MIP and the Infectious Disease Supercluster) were recently installed in Proteomics and Metabolomics Facility (http://www.pmf.colostate.edu/proteomics_services.html). This will allow excision of spots from 1D and 2D protein gels for subsequent identification by Mass Spec. Please contact Jessica Prenni (jprenni@colostate.edu) or Carol Wilusz if you are interested in this type of service.
User Fees For 2009-2010 Fiscal Year:
Although the Typhoon has been funded entirely by a Shared Instrumentation Grant from the NIH, user fees are necessary to cover the costs of the service contract and consumables. Actual fees will be adjusted depending on the number of major users and overall use of the machine.
Major Users $750 per year
(includes unlimited scanning, a copy of the software, and a key to the imager room)
Minor Users $2 per min ($20-$100 per scan)
(can only access the imager and software during normal working hours)
Links:
GE Healthcare
Invitrogen
Table:
