Michael H. Fox
Professor

Phone:(970) 491-7618
Fax: (970) 491-0623
Email: Michael.Fox@colostate.edu
Office: 485 Molecular and Radiological Biosciences Building

Degrees

• Ph.D., Biophysics, Kansas State University, Manhattan, KS
• M.S., Physics, Kansas State University, Manhattan, KS
• B.S., Physics, McPherson College, McPherson, KS

Research Interests

Dr. Fox's research program currently is centered on mutagenesis studies. His focus is developing a mammalian cell based assay for genotoxicity based on using flow cytometry to measure mutations induced by various genotoxic agents. The assay is based on hybrid Chinese hamster ovary cells which stably incorporate human chromosome 11. The CD59 gene on chromosome 11 expresses a surface antigen which can be detected with monoclonal antibodies. Mutations in the CD59 gene are expressed as loss of the CD59 protein which can also be measured with flow cytometry. This mutagenesis system is thus much more rapid than alternative mammalian mutation systems.

Currently, Dr. Fox is evaluating the ability of this system to measure mutagenesis from a variety of genotoxic agents, including ionizing radiation and a different chemicals. Other areas of interest in his lab are studies of the cell cycle and apoptosis using flow cytometry. He analyzes cell kinetics using uptake of bromodeoxyuridine to measure cell growth rates and has analyzed various physical and chemical agents for their effects on the cell cycle. He is also interested in studying the interplay between cell cycle blocks and cell cycle dependent apoptosis. He also runs a service laboratory in flow cytometry and cell sorting.

Selected Publications

• Ross CD, French CT, Keysar SB, Fox MH: Mutant spectra of irradiated CHO A(L) cells determined with multiple markers analyzed by flow cytometry. Mutat Res 2007 (Epub, in press).
• French CT, Ross CD, Keysar SB, Joshi DD, Lim CU, Fox MH: Comparison of the mutagenic potential of 17 physical and chemical agents analyzed by the flow cytometry mutation assay. Mutat Res 602:14-25, 2006.
• Lim CU, Zhang Y, Fox MH: Cell cycle dependent apoptosis and cell cycle blocks induced by hyperthermia in HL-60 cells. Int J Hyperthermia 22:77-91, 2006.
• Ross CD, Lim CU, Fox MH: Assay to measure CD59 mutations in CHO A(L) cells using flow cytometry. Cytometry A 66A:85-90, 2005.
• Zhang Y, Lim CU, Williams ES, Zhou J, Zhang Q, Fox MH, Bailey SM, Liber HL: NBS1 Knockdown by Small Interfering RNA Increases Ionizing Radiation Mutagenesis and Telomere Association in Human Cells. Cancer Res 65:5544-5553, 2005.
• Hang H, Fox MH: Analysis of the mammalian cell cycle by flow cytometry. In: Cell Cycle Checkpoint Control Protocols, Lieberman HB (ed). Humana Press Inc, Totowa, NJ, 2004.
• He L, Bradrick TD, Karpova TS, Wu X, Fox MH, Fischer R, McNally JG, Knutson JR, Grammer AC, Lipsky PE: Flow cytometric measurement of fluorescence (Förster) resonance energy transfer from cyan fluorescent protein to yellow fluorescent protein using single laser excitation at 458 nm. Cytometry 53A:39-54, 2003.
• Perez DS, Armstrong-Lea L, Fox MH, Yang RS, Campain JA: Arsenic and benzo[a]pyrene differentially alter the capacity for differentiation and growth properties of primary human epidermal keratinocytes. Toxicol Sci 76:280-290, 2003.
• Liao KH, Gustafson DL, Fox MH, Chubb LS, Reardon KF, Yang RSH: A biologically-based model of growth and senescence of Syrian hamster embryo (SHE) cells after exposure to arsenic. Environ Health Perspect 109:1207-1213, 2001.
• Fox MH: Intracellular pH Measured by ADB. In: In Living Color: Protocols in Flow Cytometry and Cell Sorting, Diamond RA and DeMaggio S (eds). Springer, New York, 2000, pp. 472-480.