Michael H. Fox
Fax: (970) 491-0623
Office: 485 Molecular and Radiological Biosciences Building
- Ph.D., Biophysics, Kansas State University, Manhattan, KS
- M.S., Physics, Kansas State University, Manhattan, KS
- B.S., Physics, McPherson College, McPherson, KS
Dr. Fox's research program currently is centered on mutagenesis studies. His focus is
developing a mammalian cell based assay for genotoxicity based on using flow cytometry to measure
mutations induced by various genotoxic agents. The assay is based on hybrid Chinese hamster ovary cells
which stably incorporate human chromosome 11. The CD59 gene on chromosome 11 expresses a surface antigen
which can be detected with monoclonal antibodies. Mutations in the CD59 gene are expressed as loss of the
CD59 protein which can also be measured with flow cytometry. This mutagenesis system is thus much more
rapid than alternative mammalian mutation systems.
Currently, Dr. Fox is evaluating the ability of this system to measure mutagenesis from a variety of
genotoxic agents, including ionizing radiation and a
different chemicals. Other areas of interest in his lab are studies of the cell cycle and apoptosis
using flow cytometry. He analyzes cell kinetics using uptake of bromodeoxyuridine
to measure cell growth rates and has analyzed various physical and chemical agents for their effects on
the cell cycle. He is also interested in studying the interplay between cell cycle blocks and cell cycle
dependent apoptosis. He also runs a service laboratory in
flow cytometry and cell sorting.
- Ross CD, French CT, Keysar SB, Fox MH: Mutant spectra of irradiated CHO A(L) cells
determined with multiple markers analyzed by flow cytometry. Mutat Res 2007 (Epub, in press).
- French CT, Ross CD, Keysar SB, Joshi DD, Lim CU, Fox MH: Comparison of the mutagenic potential
of 17 physical and chemical agents analyzed by the flow cytometry mutation assay.
Mutat Res 602:14-25, 2006.
- Lim CU, Zhang Y, Fox MH: Cell cycle dependent apoptosis and cell cycle blocks induced by
hyperthermia in HL-60 cells. Int J Hyperthermia 22:77-91, 2006.
- Ross CD, Lim CU, Fox MH: Assay to measure CD59 mutations in CHO A(L) cells using
flow cytometry. Cytometry A 66A:85-90, 2005.
- Zhang Y, Lim CU, Williams ES, Zhou J, Zhang Q, Fox MH, Bailey SM, Liber HL: NBS1 Knockdown by
Small Interfering RNA Increases Ionizing Radiation Mutagenesis and Telomere Association in Human
Cells. Cancer Res 65:5544-5553, 2005.
- Hang H, Fox MH: Analysis of the mammalian cell cycle by
flow cytometry. In: Cell Cycle Checkpoint Control Protocols, Lieberman HB (ed). Humana Press Inc,
Totowa, NJ, 2004.
- He L, Bradrick TD, Karpova TS, Wu X, Fox MH, Fischer R, McNally JG,
Knutson JR, Grammer AC, Lipsky PE: Flow cytometric measurement of fluorescence (Förster)
resonance energy transfer from cyan fluorescent protein to yellow fluorescent protein
using single laser excitation at 458 nm. Cytometry 53A:39-54, 2003.
- Perez DS, Armstrong-Lea L, Fox MH, Yang RS, Campain JA: Arsenic and
benzo[a]pyrene differentially alter the capacity for differentiation and growth properties
of primary human epidermal keratinocytes. Toxicol Sci 76:280-290, 2003.
- Liao KH, Gustafson DL, Fox MH, Chubb LS, Reardon KF, Yang RSH: A biologically-based
model of growth and senescence of Syrian hamster embryo (SHE) cells after exposure to arsenic.
Environ Health Perspect 109:1207-1213, 2001.
- Fox MH: Intracellular pH Measured by ADB. In: In Living Color: Protocols in Flow Cytometry
and Cell Sorting, Diamond RA and DeMaggio S (eds). Springer, New York, 2000, pp. 472-480.
Environmental & Radiological Health Sciences
1618 Campus Delivery
Colorado State University
Fort Collins, CO 80523
Phone: (970) 491-5222
Fax: (970) 491-0623