James K. Graham, PhD
Office: 247 Phyiology Building
I teach the andrology portion of reproductive physiology to veterinary and graduate students. I also teach endocrinology to undergraduate (BMS430) and graduate students and coordinate a team taught course on the laboratory techniques used in reproduction research.
Research Interests -- Cryobiology and Male Reproductive Physiology
My research focuses on gamete physiology, reproductive physiology and cryobiology, with an emphasis on membrane biochemistry and function. Membranes in spermatozoa are modified extensively during transit through the epididymis and the female reproductive tract prior to fertilization, or during in vitro manipulations such as cryopreservation. I am interested in understanding the membrane modifications that occur during these processes, i.e., what changes occur, when they occur and they affect cell function. Using liposome and cyclodextrin technologies, the membranes of cells (spermatozoa, embryos and cells in culture) can be changed in predetermined ways resulting in altered cell functions that can be exploited to increase cryosurvival rates, induce sperm capacitation, and many other cell functions. In addition, using proteomic and genomic approaches, we can begin to learn other aspects of sperm physiology that may play important roles in sperm fertilizing potential. Finally, understanding the relationships between reproductive endocrinology and physiology, I am interested in developing oral contraceptive technologies that can be delivered to problem feral animal populations. Current studies investigate mechanisms by which cholesterol-loaded cyclodextrins and liposomes interact with cells to induce capacitation and the acrosome reaction in sperm, thereby allowing their subsequent penetration into oocytes in vitro; and to modify cell membranes to permit more efficient cell cryosurvival. Other experiments are involved in producing, preserving and enabling oral uptake of molecules that can inhibit the fertility of targeted problem feral animals.
For additional information on current projects, consult the page on Gamete Biology and Andrology.
Mocé E, Blanch E, Tomás C, Graham JK. 2010. Use of cholesterol in sperm cryopreservation: Present moment and perspectives to future. Reprod Dom Anim 45(Suppl 2):57-66.
Moraes EA, Graham JK, Torres CAA, Meyers M, Spizziri B. 2010. Delivering cholesterol or cholestanol to bull sperm membranes improves cryosurvival. Anim Reprod Sci 118:148-154.
Spizziri BE, Fox MH, Bruemmer JE, Squires EL, Graham JK 2010. Cholesterol-loaded-cyclodextrins and fertility potential of stallion spermatozoa. Anim Reprod Sci 118:255-264.
Purdy PH, Mocé E, Stobart R, Murdoch WJ, Moss GE, Ramsey S, Graham JK, Blackburn HD. 2010. The fertility of ram sperm held for 24h at 5 degrees prior to cryopreservation. Anim Reprod Sci 118:231-235.
Mocé E, Purdy PH, Graham JK. 2010. Treating ram sperm with cholesterol-loaded cyclodextrins improves cryosurvival. Anim Reprod Sci 118:236-247.
Glazer AI, Mullen SF, Liu J, Benson JD, Critser JK, Squires EL, Graham JK. 2009. Osmotic tolerance limits and membrane permeability characteristics of stallion spermatozoa treated with cholesterol. Cryobiology 59:201-206.
Amorim EA, Graham JK, Spizziri B, Meyers M, Torres CA. 2009. Effect of cholesterol or cholesteryl conjugates on the cryosurvival of bull sperm. Cryobiology 58:210-214.
Yoder CA, Graham JK, Miller LA, Bynum KS, Johnston JJ, Goodall MJ. 2006. Effect of method of delivering Nicarbazin to mallards on plasma 4,4'-dinitrocarbanilide levels and reproduction. Poultry Sci 85:1442-1448.
Yoder CA, Graham JK, Miller LA. 2006. Molecular effects of Nicarbazin on avian reproduction. Poultry Sci 85:1285-1293.
Yoder CA, Graham JK, Miller LA, Bynum KS, Johnston JJ, Goodall MJ. 2006. Evaluation of Nicarbazin as a potential waterfowl contraceptive using mallards as a model. Poultry Sci 85:1275-1284.
Mocé E, Graham JK, Schenk JL. 2006. Effect of sex-sorting on the ability of fresh and cryopreserved bull sperm to undergo an acrosome reaction. Theriogenology 66:929-936.
Mocé E, Graham JK. 2006. Cholesterol-loaded cyclodextrins added to raw bull ejaculates improve sperm cryosurvival. J Anim Sci 84:826-833.
Purdy PH, Fox MH, Graham JK. 2005. The fluidity of Chinese hamster ovary cell and bull sperm membranes after cholesterol addition. Cryobiology 51:102-112.
Purdy PH, Graham JK. 2004. Effect of cholesterol-loaded cyclodextrin on the cryosurvival of bull sperm. Cryobiology 48:36-45.
Purdy PH, Graham JK. 2004. Effect of adding cholesterol to bull sperm membranes on sperm capacitation, the acrosome reaction and fertility. Biol Reprod 71:522-527.
Graham JK. 2001. Assessment of sperm quality: A flow cytometric approach. Anim Reprod Sci 68:239-248.
Dewit M, Marley WS, Graham JK. 2000. Fertilizing potential of mouse spermatozoa cryopreserved in a medium containing whole eggs. Cryobiology 40:36-45.
Graham JK, Kunze E Hammerstedt RH. 1990. Analysis of sperm cell viability, acrosomal integrity and mitochondrial function using flow cytometry. Biol Reprod 43:55-64.