Gerrit J. Bouma, PhDAssistant Professor Phone: 970-491-8738 Fax: 970-491-3557 Email: Gerrit.Bouma@colostate.edu Member Education |
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Research Interests -- Mammalian Gonad Development and Differentiation
The mammalian bipotential, indifferent gonadal primordium has the ability to give rise to two distinct organs, an ovary in XX individuals and a testis in XY individuals. Correct timing and expression of the genetic factors controlling this developmental process are crucial for proper differentiation in adult gonads. A number of genes have been identified in fetal testis development and differentiation. Notably, the early, brief expression of the mammalian testis determining gene Sry and the subsequent up-regulation of Sox9 appear to be crucial events in initiating fetal testicular organogenesis. However, the genetic control regulating fetal gonad differentiation remains largely unknown. An ovarian determining gene remains yet to be discovered, and the majority of human sex reversal cases are unexplained. My research interest is the developmental biology of gonadal sex determination, and my goal is to identify and elucidate the transcriptional network controlling fetal gonad development and differentiation.
Recent Publications
Hess RA, Bunick D, Lubahn DB, Zhou Q, Bouma J. 2000. Morphological changes in efferent ductules and epididymis in estrogen receptor- knockout mice. J Androl 21:107-121.
Nakai M, Bouma J, Nie R, Zhou Q, Carnes K, Lubahn DB, Hess RA. 2001. Morphological analysis of endocytosis in efferent ductules of estrogen receptor- knockout male mouse. Anat Rec 263:10-18.
Lane RA, Bouma J, Northcott CA. 2001. Comparing the clastogenic potential of atrazine with caffeine using Chinese hamster ovary (CHO) cells. Toxicol Lett 121:69-78.
Bouma J, Nagler JJ. 2001. Estrogen receptor- protein localization in the testis of the rainbow trout (Oncorhynchus mykiss) during different stages of the reproductive cycle. Biol Reprod 65:60-65.
Nagler JJ, Bouma J, Thorgaard GH, Dauble DD. 2001. High incidence of a male-specific genetic marker in phenotypic female chinook salmon from the Columbia River. Environ Health Perspect 109:67-69.
Bouma J, Cloud JG, Nagler JJ. 2003. In vitro effects of estradiol-17ß on myoid and fibroblastic cell proliferation in the immature rainbow trout testis. Fish Physiol Biochem 28:191-192.
Bouma GJ, Hart GT, Washburn LL, Recknagel AK, Eicher EM. 2004. Using real time RT-PCR analysis to determine multiple gene expression patterns during XX and XY mouse fetal gonad development. Gene Expr Patterns 5:141-149.
Bouma GJ, Albrecht KH, Washburn LL, Recknagel AK, Churchill GA, Eicher EM. 2005. Gonadal sex reversal in mutant Dax1 XY mice: A failure to up-regulate Sox9 in pre-Sertoli cells. Development 132:3045-3054.
Bouma GJ, Cloud JG, Nagler JJ. 2005. An in vitro system for the long-term tissue culture of juvenile rainbow trout (Oncorhynchus mykiss) testis. J Exp Zool 303A:698-703.
Bouma J, Cloud JG. 2005. Sertoli cell biology in fish and amphibians. In: Sertoli Cell Biology, Skinner MK, Grisold MD (eds). Elsevier Academic Press, San Diego, CA, pp 71-79.
Bouma GJ, Affourtit JP, Bult CJ, Eicher EM. 2007. Transcriptional profile of mouse pre-granulosa and Sertoli cells isolated from early-differentiated fetal gonads. Gene Expression Patterns 7:113-123.
Bouma GJ, Washburn LL, Albrecht KH, Eicher EM. 2007. Correct dosage of Fog2 and Gata4 transcription factors is critical for fetal testis development in mice. Proc Natl Acad Sci USA 104 (98):14994-14999.
