Gerrit J. Bouma, PhDAssociate Professor Phone: 970-491-8738 Fax: 970-491-3557 Email: Gerrit.Bouma@colostate.edu Member Education |
 |
Research Interests -- Genetic and Molecular Control of Fetal Gonad Development and Adult Reproductive Function
A variety of adult reproductive diseases, such as testicular dysgenesis syndrome, polycystic ovary syndrome, and testicular and ovarian cancer have been linked to abnormal fetal gonadal development and/or differentiation. A better understanding regarding the normal processes controlling gonad development, and the effects of exogenous exposures on gene expression and function during fetal gonadal programming, can provide much needed insight into adult onset of reproductive disorders. The main focus of our research is to obtain insight into the genetic and molecular factors that underlie fetal and adult cell differentiation and function in reproductive tissues.
Projects in my laboratory include (1) studying the role of the transcription factor GATA4 in mammalian fetal ovarian development; (2) investigating the role of microRNAs in fetal and adult ovarian function and disease; and (3) examining the role of stem cell factors in reproductive tissue development and differentiation.
Recent Publications
Hess RA, Bunick D, Lubahn DB, Zhou Q, Bouma J. 2000. Morphological changes in efferent ductules and epididymis in estrogen receptor- knockout mice. J Androl 21:107-121.
Nakai M, Bouma J, Nie R, Zhou Q, Carnes K, Lubahn DB, Hess RA. 2001. Morphological analysis of endocytosis in efferent ductules of estrogen receptor- knockout male mouse. Anat Rec 263:10-18.
Lane RA, Bouma J, Northcott CA. 2001. Comparing the clastogenic potential of atrazine with caffeine using Chinese hamster ovary (CHO) cells. Toxicol Lett 121:69-78.
Bouma J, Nagler JJ. 2001. Estrogen receptor- protein localization in the testis of the rainbow trout (Oncorhynchus mykiss) during different stages of the reproductive cycle. Biol Reprod 65:60-65.
Nagler JJ, Bouma J, Thorgaard GH, Dauble DD. 2001. High incidence of a male-specific genetic marker in phenotypic female chinook salmon from the Columbia River. Environ Health Perspect 109:67-69.
Bouma J, Cloud JG, Nagler JJ. 2003. In vitro effects of estradiol-17ß on myoid and fibroblastic cell proliferation in the immature rainbow trout testis. Fish Physiol Biochem 28:191-192.
Bouma GJ, Hart GT, Washburn LL, Recknagel AK, Eicher EM. 2004. Using real time RT-PCR analysis to determine multiple gene expression patterns during XX and XY mouse fetal gonad development. Gene Expr Patterns 5:141-149.
Bouma GJ, Albrecht KH, Washburn LL, Recknagel AK, Churchill GA, Eicher EM. 2005. Gonadal sex reversal in mutant Dax1 XY mice: A failure to up-regulate Sox9 in pre-Sertoli cells. Development 132:3045-3054.
Bouma GJ, Cloud JG, Nagler JJ. 2005. An in vitro system for the long-term tissue culture of juvenile rainbow trout (Oncorhynchus mykiss) testis. J Exp Zool 303A:698-703.
Bouma J, Cloud JG. 2005. Sertoli cell biology in fish and amphibians. In: Sertoli Cell Biology, Skinner MK, Grisold MD (eds). Elsevier Academic Press, San Diego, CA, pp 71-79.
Bouma GJ, Affourtit JP, Bult CJ, Eicher EM. 2007. Transcriptional profile of mouse pre-granulosa and Sertoli cells isolated from early-differentiated fetal gonads. Gene Expression Patterns 7:113-123.
Bouma GJ, Washburn LL, Albrecht KH, Eicher EM. 2007. Correct dosage of Fog2 and Gata4 transcription factors is critical for fetal testis development in mice. Proc Natl Acad Sci USA 104 (98):14994-14999.
Bouma GJ, Hudson QJ, Washburn LL, Eicher EM. 2010. New candidate genes identified for controlling mouse gonadal sex determination and the early stage of granulose and Sertoli cell differentiation. Biol Reprod 82:380-389.
Ashley RL, Henkes LE, Bouma GJ, Pru JK, Hansen TR. 2010. Deletion of the Isg15 gene results in up-regulation of decidual cell survival genes and down-regulation of adhesion genes: implication for regulation by IL-1beta. Endocrinology 151:4527-4536.
Torley KJ, da Silveira JC, Smith P, Anthony RV, Veeramachaneni DNR, Winger QA, Bouma GJ. 2011. Expression of miRNAs in ovine fetal gonads: potential role in gonadal differentiation. Reprod Biol Endocrinol 9:2.
Zhang Q, Bouma GJ, McClellan K, Tobet S. 2012. Hypothalamic expression of snoRNA Snord116 is consistent with a link to the hyperphagia and obesity symptoms of Prader-Willi syndrome. Int J Dev Neurosci [Epub ahead of print].
Correa SM, Washburn LL, Kahlon RS, Musson MC, Bouma GJ, Eicher EM, Albrecht KH. 2012. Sex reversal in C57BL/6J XY mice caused by increased expression of ovarian genes and insufficient activation of the testis determining pathway. PLoS Genet 8(4):e1002569.
da Silveira JC, Veeramachaneni DN, Winger QA, Carnevale EM,Bouma GJ. 2012. Cell-secreted vesicles in equine ovarian follicular fluid contain miRNAs and proteins: a possible new form of cell communication within the ovarian follicle. Biol Reprod 86:71
