| Type of
Hormone: Protein
Purpose of Test:
To determine serum concentration of FSH in order to obtain information
about
reproductive function. Reasons to perform a FSH test vary with the
species and include:
 |
to check
for neuter/spay status |
 |
to aid in
the diagnosis of reproductive disorders |
Description of test and procedures:
The test is a radioimmunoassay performed with in-house reagents.
The radioimmunoassay technique is based on the competitive binding of the
serum FSH and a radiolabeled FSH preparation. They compete for
binding to an antibody specific for FSH. The antibody-bound
radiolabeled FSH is separated and the quantity is determined by counting
on a gamma spectrometer. Results for the unknown are read from a curve
prepared by plotting results for a set of known standards. Serum with a
pre-determined concentration are included in the assay for quality control
purposes.
Sample Needed and Procedures for
Submittal: Submit at least
1mL of serum on a cold pack.
It may be possible to perform this analysis with less serum, but
laboratory approval is required.
Schedule For Running Test:
Assay preparation begins Wednesday morning and results are reported the
following Monday afternoon.
Interpretation of Results:
Results should be interpreted by a veterinary clinician or researcher
familiar with reproductive function in the species being tested.
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